Restriction map for
pF1KSDB0040
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
Alw44I ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I BalI
BamHI BcgI BciVI BclI BfrI
BlpI Bpu1102I BsiEI BsiWI BsmI
BspDI BspHI BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MfeI MluI
MluNI MscI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tru9I Tsp509I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 795 GACGTC GACGT^C
AccI 538 GTCTAC GT^[AC][GT]AC
AccIII 362 TCCGGA T^CCGGA
AflIII 482 ACATGT A^C[GA][CT]GT
AlwI 945 GATCC GGATC(4/5)
AlwNI 490 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 657 CCTGAGG CC^T[ACGT]AGG
Aor51HI 179 AGCGCT AGC^GCT
AspI 532 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1541 TGCGCA TGC^GCA
AvrII 231 CCTAGG C^CTAGG
BbrPI 1182 CACGTG CAC^GTG
BbvCI 423 CCTCAGC CCTCAGC(-5/-2)
BglII 734 AGATCT A^GATCT
BlnI 231 CCTAGG C^CTAGG
BsaBI 462 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 362 TCCGGA [AT]^CCGG[AT]
BseAI 362 TCCGGA T^CCGGA
BsmBI 214 GAGACG CGTCTC(1/5)
Bsp1407I 497 TGTACA T^GTACA
BspEI 362 TCCGGA T^CCGGA
BspLU11I 482 ACATGT A^CATGT
BsrDI 1409 GCAATG GCAATG(2/0)
BsrFI 1169 ACCGGC [GA]^CCGG[CT]
BsrGI 497 TGTACA T^GTACA
BstAPI 921 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1679 CCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 657 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1169 ACCGGC [GA]^CCGG[CT]
DraII 491 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 795 GACGTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 179 AGCGCT AGC^GCT
Eco81I 657 CCTGAGG CC^T[ACGT]AGG
EcoNI 701 CCTTCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 491 AGGACCT [GA]G^G[ACGT]CC[CT]
FspI 1541 TGCGCA TGC^GCA
KspI 993 CCGCGG CCGC^GG
MaeIII 510 GTCAC ^GT[ACGT]AC
MamI 462 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 362 TCCGGA T^CCGGA
NcoI 1685 CCATGG C^CATGG
PflFI 532 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1182 CACGTG CAC^GTG
PmlI 1182 CACGTG CAC^GTG
PpuMI 491 AGGACCT [GA]G^G[AT]CC[CT]
PstI 937 CTGCAG CTGCA^G
SacI 301 GAGCTC GAGCT^C
SacII 993 CCGCGG CCGC^GG
SexAI 1391 ACCTGGT A^CC[AT]GGT
SfcI 937 CTGCAG C^T[GA][CT]AG
SphI 41 GCATGC GCATG^C
SspBI 497 TGTACA T^GTACA
TfiI 1054 GAATC G^A[AT]TC
Tsp45I 510 GTCAC ^GT[GC]AC
Tth111I 532 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AhdI 366 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
719 GACTCAAGGTC
AspEI 366 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
719 GACTCAAGGTC
AvaI 196 CCCGAG C^[CT]CG[GA]G
1195 CCCGAG
BbeI 337 GGCGCC GGCGC^C
1145 GGCGCC
BglI 776 GCCTCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1554 GCCCGACTGGC
BsaAI 1182 CACGTG [CT]AC^GT[GA]
1479 CACGTA
BsaI 373 GGTCTC GGTCTC(1/5)
1395 GGTCTC
BseRI 36 GAGGAG GAGGAG(10/8)
292 GAGGAG
BsgI 640 CTGCAC GTGCAG(16/14)
1324 GTGCAG
BsoBI 196 CCCGAG C^[CT]CG[GA]G
1195 CCCGAG
BstYI 734 AGATCT [GA]^GATC[CT]
944 AGATCC
DraIII 1066 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
1318 CACACGGTG
Eam1105I 366 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
719 GACTCAAGGTC
EarI 352 CTCTTC CTCTTC(1/4)
1717 CTCTTC
EcoT14I 231 CCTAGG C^C[AT][AT]GG
1685 CCATGG
KasI 337 GGCGCC G^GCGCC
1145 GGCGCC
Ksp632I 352 CTCTTC CTCTTC(1/4)
1717 CTCTTC
MflI 734 AGATCT [GA]^GATC[CT]
944 AGATCC
NarI 337 GGCGCC GG^CGCC
1145 GGCGCC
SfoI 337 GGCGCC GGC^GCC
1145 GGCGCC
SmlI 505 CTCAAG C^T[CT][GA]AG
721 CTCAAG
StyI 231 CCTAGG C^C[AT][AT]GG
1685 CCATGG
XcmI 47 CCAGCCCCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
74 CCATCCTCCTGCTGG
XhoII 734 AGATCT [GA]^GATC[CT]
944 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I
Van91I