Restriction map for
pF1KSDB0037
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# NO CUT ENZYMES ( 151 enzymes) #
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AccIII AclI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvaII
AviII AvrII BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII CfoI Cfr13I ClaI CpoI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HindIII HpaI KasI KspI
MluI MroI MspA1I NarI NciI
NcoI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 649 GACGTC GACGT^C
Acc65I 1146 GGTACC G^GTACC
AccI 1288 GTAGAC GT^[AC][GT]AC
AccII 1018 CGCG CG^CG
AcyI 649 GACGTC G[GA]^CG[CT]C
Asp718I 1146 GGTACC G^GTACC
AvaI 734 CCCGAG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BamHI 1238 GGATCC G^GATCC
BanII 1218 GGGCTC G[GA]GC[CT]^C
BbvCI 1001 CCTCAGC CCTCAGC(-5/-2)
BglII 809 AGATCT A^GATCT
BsaHI 649 GACGTC G[GA]^CG[CT]C
BsaJI 1234 CCTCGG C^C[ACGT][ACGT]GG
BseRI 940 GAGGAG GAGGAG(10/8)
BsmFI 426 GTCCC GGGAC(10/14)
BsmI 491 GAATGC GAATGC(1/-1)
BsoBI 734 CCCGAG C^[CT]CG[GA]G
BsrFI 52 GCCGGC [GA]^CCGG[CT]
BstF5I 722 GGATG GGATG(2/0)
BstUI 1018 CGCG CG^CG
Cfr10I 52 GCCGGC [GA]^CCGG[CT]
DraI 369 TTTAAA TTT^AAA
EcoNI 148 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 722 GGATG GGATG(9/13)
HapII 53 CCGG C^CGG
Hin1I 649 GACGTC G[GA]^CG[CT]C
HpaII 53 CCGG C^CGG
KpnI 1146 GGTACC GGTAC^C
MfeI 557 CAATTG C^AATTG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspI 53 CCGG C^CGG
MunI 557 CAATTG C^AATTG
MvnI 1018 CGCG CG^CG
NaeI 52 GCCGGC GCC^GGC
NdeI 821 CATATG CA^TATG
NgoMIV 52 GCCGGC G^CCGGC
NlaIII 207 CATG CATG^
PflMI 657 CCACGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 794 AGTACT AGT^ACT
SfaNI 292 GATGC GCATC(5/9)
SfcI 1286 CTGTAG C^T[GA][CT]AG
TaqI 447 TCGA T^CGA
TthHB8I 447 TCGA T^CGA
Van91I 657 CCACGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 690 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 323 CCGC CCGC(-3/-1)
680 GCGG
BanI 115 GGCACC G^G[CT][GA]CC
1146 GGTACC
BbsI 1123 GAAGAC GAAGAC(2/6)
652 GTCTTC
BmyI 832 GTGCCC G[AGT]GC[ACT]^C
1218 GGGCTC
BpuAI 1123 GAAGAC GAAGAC(2/6)
652 GTCTTC
BsaI 412 GAGACC GGTCTC(1/5)
634 GGTCTC
Bsp1286I 832 GTGCCC G[AGT]GC[ACT]^C
1218 GGGCTC
BsrDI 80 CATTGC GCAATG(2/0)
739 GCAATG
BsrI 1143 ACTGG ACTGG(1/-1)
496 CCAGT
BssKI 148 CCTGG ^CC[ACGT]GG
1179 CCTGG
BstNI 148 CCTGG CC^[AT]GG
1179 CCTGG
Cac8I 52 GCCGGC GC[ACGT]^[ACGT]GC
258 GCTGGC
EaeI 2 TGGCCA [CT]^GGCC[GA]
50 TGGCCG
EarI 899 CTCTTC CTCTTC(1/4)
328 GAAGAG
EcoRII 148 CCTGG ^CC[AT]GG
1179 CCTGG
HphI 168 GGTGA GGTGA(8/7)
666 GGTGA
Ksp632I 899 CTCTTC CTCTTC(1/4)
328 GAAGAG
MslI 417 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
964 CACAGTAGTG
MvaI 148 CCTGG CC^[AT]GG
1179 CCTGG
PleI 39 GACTC GAGTC(4/5)
897 GACTC
ScrFI 148 CCTGG CC^[ACGT]GG
1179 CCTGG
Tsp45I 400 GTGAC ^GT[GC]AC
971 GTGAC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
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AcsI AfaI AluI AlwI ApoI
BbvI BfaI BsaBI BsiYI BslI
BsmAI BstYI DdeI DpnII Fnu4HI
HaeIII HinfI ItaI MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MwoI NdeII NlaIV
RsaI Sau3AI TfiI Tru9I TseI
Tsp509I TspRI XhoII