Restriction map for
pF1KSDB0033
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII AccI AclI AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AspI AvrII BamHI BbeI
BbsI BcgI BclI BglI BlnI
BpuAI BsmI BspDI BspHI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI KasI KspI MfeI MluI
MunI NarI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
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Acc65I 21 GGTACC G^GTACC
AflII 1708 CTTAAG C^TTAAG
Alw44I 682 GTGCAC G^TGCAC
AocI 2457 CCTGAGG CC^T[ACGT]AGG
ApaI 3081 GGGCCC GGGCC^C
ApaLI 682 GTGCAC G^TGCAC
Asp718I 21 GGTACC G^GTACC
BbvCI 1023 CCTCAGC CCTCAGC(-5/-2)
BciVI 1542 GGATAC GTATCC(6/5)
BfaI 2630 CTAG C^TAG
BfrI 1708 CTTAAG C^TTAAG
BlpI 912 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 912 GCTGAGC GC^T[ACGT]AGC
BsaBI 2142 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 1124 CGTACG C^GTACG
BsrDI 974 GCAATG GCAATG(2/0)
Bsu36I 2457 CCTGAGG CC^T[ACGT]AGG
CelII 912 GCTGAGC GC^T[ACGT]AGC
Eco81I 2457 CCTGAGG CC^T[ACGT]AGG
HaeII 3 GGCGCT [GA]GCGC^[CT]
KpnI 21 GGTACC GGTAC^C
MaeI 2630 CTAG C^TAG
MamI 2142 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 104 CCATGG C^CATGG
NdeI 298 CATATG CA^TATG
PflMI 723 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 750 GACTC GAGTC(4/5)
SmaI 1501 CCCGGG CCC^GGG
Van91I 723 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1598 CCAGGAAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1501 CCCGGG C^CCGGG
XmaI 1501 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccIII 569 TCCGGA T^CCGGA
1700 TCCGGA
Asp700I 1276 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2412 GAACGCCTTC
BbrPI 2052 CACGTG CAC^GTG
2992 CACGTG
BsaAI 2052 CACGTG [CT]AC^GT[GA]
2992 CACGTG
BseAI 569 TCCGGA T^CCGGA
1700 TCCGGA
Bsp1407I 1154 TGTACA T^GTACA
2940 TGTACA
BspEI 569 TCCGGA T^CCGGA
1700 TCCGGA
BsrBI 423 GAGCGG CCGCTC(-3/-3)
700 GAGCGG
BsrGI 1154 TGTACA T^GTACA
2940 TGTACA
BstAPI 2604 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2814 GCAGCTGCTGC
DraIII 1930 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2490 CACCTCGTG
DrdI 847 GACTGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2854 GACGCCAAAGTC
EagI 1902 CGGCCG C^GGCCG
2065 CGGCCG
EclXI 1902 CGGCCG C^GGCCG
2065 CGGCCG
Eco52I 1902 CGGCCG C^GGCCG
2065 CGGCCG
EcoRI 1621 GAATTC G^AATTC
3180 GAATTC
HindIII 70 AAGCTT A^AGCTT
2006 AAGCTT
MroI 569 TCCGGA T^CCGGA
1700 TCCGGA
MseI 1460 TTAA T^TAA
1709 TTAA
MslI 39 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
603 CATGGATGTG
NaeI 290 GCCGGC GCC^GGC
1978 GCCGGC
NgoMIV 290 GCCGGC G^CCGGC
1978 GCCGGC
PmaCI 2052 CACGTG CAC^GTG
2992 CACGTG
PmlI 2052 CACGTG CAC^GTG
2992 CACGTG
PpuMI 272 GGGACCT [GA]G^G[AT]CC[CT]
905 AGGACCT
SapI 1341 GCTCTTC GCTCTTC(1/4)
2682 GCTCTTC
SexAI 797 ACCTGGT A^CC[AT]GGT
1931 ACCAGGT
SspBI 1154 TGTACA T^GTACA
2940 TGTACA
Tru9I 1460 TTAA T^TAA
1709 TTAA
XmnI 1276 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2412 GAACGCCTTC
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# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII AviII BalI BanI
BanII BbvI BcnI BglII BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII