Restriction map for
pF1KSDB0032
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BmyI BsaAI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI Bsp1286I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI KasI
KpnI KspI MluI MroI MslI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 911 GACGTC GACGT^C
AccII 691 CGCG CG^CG
AcyI 911 GACGTC G[GA]^CG[CT]C
AvaI 626 CTCGAG C^[CT]CG[GA]G
BalI 810 TGGCCA TGG^CCA
BbsI 1062 GAAGAC GAAGAC(2/6)
BlpI 85 GCTCAGC GC^T[ACGT]AGC
BpmI 1379 CTCCAG CTGGAG(16/14)
Bpu1102I 85 GCTCAGC GC^T[ACGT]AGC
BpuAI 1062 GAAGAC GAAGAC(2/6)
BsaBI 1156 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 911 GACGTC G[GA]^CG[CT]C
BseRI 467 CTCCTC GAGGAG(10/8)
BsgI 28 GTGCAG GTGCAG(16/14)
BsmAI 1149 GTCTC GTCTC(1/5)
BsmI 902 GAATGC GAATGC(1/-1)
BsoBI 626 CTCGAG C^[CT]CG[GA]G
Bsp1407I 302 TGTACA T^GTACA
BspMI 930 GCAGGT ACCTGC(4/8)
BsrGI 302 TGTACA T^GTACA
BssHII 690 GCGCGC G^CGCGC
BstUI 691 CGCG CG^CG
CelII 85 GCTCAGC GC^T[ACGT]AGC
DraII 966 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 810 TGGCCA [CT]^GGCC[GA]
EarI 734 CTCTTC CTCTTC(1/4)
EcoO109I 966 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1136 GAATTC G^AATTC
EcoT14I 813 CCAAGG C^C[AT][AT]GG
Hin1I 911 GACGTC G[GA]^CG[CT]C
HincII 163 GTTAAC GT[CT]^[GA]AC
HindII 163 GTTAAC GT[CT]^[GA]AC
HpaI 163 GTTAAC GTT^AAC
Ksp632I 734 CTCTTC CTCTTC(1/4)
MaeII 912 ACGT A^CGT
MamI 1156 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1292 CAATTG C^AATTG
MluNI 810 TGGCCA TGG^CCA
MscI 810 TGGCCA TGG^CCA
MunI 1292 CAATTG C^AATTG
MvnI 691 CGCG CG^CG
NdeI 607 CATATG CA^TATG
NlaIV 870 GGAGCC GG[ACGT]^[ACGT]CC
NspI 299 GCATGT [GA]CATG^[CT]
PaeR7I 626 CTCGAG C^TCGAG
PpuMI 966 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 699 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SspBI 302 TGTACA T^GTACA
SspI 1406 AATATT AAT^ATT
StyI 813 CCAAGG C^C[AT][AT]GG
Tsp45I 1221 GTCAC ^GT[GC]AC
XhoI 626 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 834 CCGC CCGC(-3/-1)
1182 CCGC
AfaI 303 GTAC GT^AC
1384 GTAC
AlwI 253 GATCC GGATC(4/5)
1050 GGATC
Asp700I 448 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1319 GAACATCTTC
BfaI 404 CTAG C^TAG
1210 CTAG
BglII 335 AGATCT A^GATCT
463 AGATCT
DraI 766 TTTAAA TTT^AAA
1347 TTTAAA
HapII 797 CCGG C^CGG
874 CCGG
HindIII 107 AAGCTT A^AGCTT
615 AAGCTT
HpaII 797 CCGG C^CGG
874 CCGG
MaeI 404 CTAG C^TAG
1210 CTAG
MspA1I 31 CAGCTG C[AC]G^C[GT]G
1331 CAGCTG
MspI 797 CCGG C^CGG
874 CCGG
NlaIII 53 CATG CATG^
300 CATG
PflMI 802 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1093 CCAAAGCCTGG
PleI 390 GACTC GAGTC(4/5)
36 GAGTC
PvuII 31 CAGCTG CAG^CTG
1331 CAGCTG
RsaI 303 GTAC GT^AC
1384 GTAC
SmlI 626 CTCGAG C^T[CT][GA]AG
1057 CTTGAG
Van91I 802 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1093 CCAAAGCCTGG
XmnI 448 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1319 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AluI ApoI AvaII BbvI
BsaJI BsiYI BslI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII PstI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII