Restriction map for
pF1KSDB0010
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BbeI BbrPI BcgI
BclI BfrI BlnI BsaBI BsaHI
BsaI BseAI BsiEI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrGI BstBI ClaI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MunI NarI NheI
NotI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI SacII SalI SapI
ScaI SexAI SfaNI SfoI SfuI
SgrAI SmaI SnaBI SpeI SspBI
SspI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 130 GGTACC G^GTACC
AccI 1336 GTATAC GT^[AC][GT]AC
AcsI 1628 GAATTC [GA]^AATT[CT]
ApoI 1628 GAATTC [GA]^AATT[CT]
Asp718I 130 GGTACC G^GTACC
AspHI 934 GAGCTC G[AT]GC[AT]^C
AspI 540 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1436 GGATCC G^GATCC
BbvCI 300 CCTCAGC CCTCAGC(-5/-2)
BglII 1505 AGATCT A^GATCT
BlpI 823 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 823 GCTCAGC GC^T[ACGT]AGC
BsaAI 640 TACGTG [CT]AC^GT[GA]
BsgI 991 GTGCAG GTGCAG(16/14)
BsiHKAI 934 GAGCTC G[AT]GC[AT]^C
BsiWI 151 CGTACG C^GTACG
BsmBI 1563 GAGACG CGTCTC(1/5)
BspHI 1001 TCATGA T^CATGA
BsrFI 848 GCCGGC [GA]^CCGG[CT]
BssHII 69 GCGCGC G^CGCGC
BssSI 841 CACGAG CACGAG(-5/-1)
Bst1107I 1336 GTATAC GTA^TAC
BstZ17I 1336 GTATAC GTA^TAC
CelII 823 GCTCAGC GC^T[ACGT]AGC
Cfr10I 848 GCCGGC [GA]^CCGG[CT]
CpoI 11 CGGACCG CG^G[AT]CCG
DsaI 683 CCATGG C^C[GA][CT]GG
EarI 532 GAAGAG CTCTTC(1/4)
EcoRI 1628 GAATTC G^AATTC
KpnI 130 GGTACC GGTAC^C
Ksp632I 532 GAAGAG CTCTTC(1/4)
MseI 1352 TTAA T^TAA
NaeI 848 GCCGGC GCC^GGC
NcoI 683 CCATGG C^CATGG
NgoMIV 848 GCCGGC G^CCGGC
NruI 177 TCGCGA TCG^CGA
PflFI 540 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1426 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 403 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1001 TCATGA T^CATGA
RsrII 11 CGGACCG CG^G[AT]CCG
SacI 934 GAGCTC GAGCT^C
SbfI 1452 CCTGCAGG CCTGCA^GG
SfiI 1595 GGCCAAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1452 CCTGCAGG CCTGCA^GG
StuI 1481 AGGCCT AGG^CCT
TfiI 214 GAATC G^A[AT]TC
Tru9I 1352 TTAA T^TAA
Tsp509I 1629 AATT ^AATT
Tth111I 540 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1426 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 348 CCTGAGG CC^T[ACGT]AGG
1213 CCTGAGG
Aor51HI 1109 AGCGCT AGC^GCT
1442 AGCGCT
AvaI 703 CCCGAG C^[CT]CG[GA]G
1238 CCCGAG
BalI 677 TGGCCA TGG^CCA
1718 TGGCCA
BbsI 710 GTCTTC GAAGAC(2/6)
1258 GTCTTC
BciVI 41 GGATAC GTATCC(6/5)
1059 GGATAC
BcnI 9 CCCGG CC^[GC]GG
197 CCGGG
BglI 679 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1596 GCCAAGAGGGC
BpuAI 710 GTCTTC GAAGAC(2/6)
1258 GTCTTC
BsaWI 38 ACCGGA [AT]^CCGG[AT]
265 TCCGGT
BsoBI 703 CCCGAG C^[CT]CG[GA]G
1238 CCCGAG
BsrDI 225 CATTGC GCAATG(2/0)
1538 CATTGC
BstAPI 954 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1462 GCAGACCCTGC
BstEII 1203 GGTCACC G^GT[ACGT]ACC
1390 GGTGACC
BstPI 1203 GGTCACC G^GT[ACGT]ACC
1390 GGTGACC
BstXI 491 CCACACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1580 CCAACAGCCTGG
Bsu36I 348 CCTGAGG CC^T[ACGT]AGG
1213 CCTGAGG
Eco47III 1109 AGCGCT AGC^GCT
1442 AGCGCT
Eco81I 348 CCTGAGG CC^T[ACGT]AGG
1213 CCTGAGG
EcoO65I 1203 GGTCACC G^GT[ACGT]ACC
1390 GGTGACC
MaeII 150 ACGT A^CGT
641 ACGT
MluNI 677 TGGCCA TGG^CCA
1718 TGGCCA
MscI 677 TGGCCA TGG^CCA
1718 TGGCCA
MslI 856 CATATGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1350 CATTAAGGTG
NciI 9 CCCGG CC^[GC]GG
197 CCGGG
NdeI 422 CATATG CA^TATG
856 CATATG
PpuMI 188 AGGTCCT [GA]G^G[AT]CC[CT]
698 GGGTCCC
SmlI 1021 CTCAAG C^T[CT][GA]AG
1033 CTCAAG
SphI 62 GCATGC GCATG^C
73 GCATGC
TaqI 714 TCGA T^CGA
1245 TCGA
TthHB8I 714 TCGA T^CGA
1245 TCGA
XcmI 421 CCATATGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1706 CCAAAGGGGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaII BanI BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI StyI TseI
Tsp45I TspRI XhoII