##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaWI       BseAI       
BsiWI       BsmBI       BsmI        BspEI       BspHI       
BspLU11I    BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     CelII       CpoI        DraI        
Eam1105I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        MfeI        MluI        MroI        
MunI        NcoI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SapI        SbfI        SfuI        
SmlI        SnaBI       SpeI        Sse8387I    SspI        
SwaI        TfiI        Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AocI         1916  CCTCAGG              CC^T[ACGT]AGG
BalI          326  TGGCCA               TGG^CCA
BsaAI        1225  TACGTG               [CT]AC^GT[GA]
BsaBI         228  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2439  GGTCTC               GGTCTC(1/5)
Bsp1407I      419  TGTACA               T^GTACA
BspDI        2506  ATCGAT               AT^CGAT
BsrGI         419  TGTACA               T^GTACA
Bsu36I       1916  CCTCAGG              CC^T[ACGT]AGG
ClaI         2506  ATCGAT               AT^CGAT
EarI         2064  CTCTTC               CTCTTC(1/4)
Eco81I       1916  CCTCAGG              CC^T[ACGT]AGG
FseI           24  GGCCGGCC             GGCCGG^CC
Ksp632I      2064  CTCTTC               CTCTTC(1/4)
MamI          228  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         326  TGGCCA               TGG^CCA
MscI          326  TGGCCA               TGG^CCA
MseI         1814  TTAA                 T^TAA
NheI         1188  GCTAGC               G^CTAGC
NspI          435  GCATGC               [GA]CATG^[CT]
ScaI         1067  AGTACT               AGT^ACT
SexAI        2252  ACCAGGT              A^CC[AT]GGT
SgrAI        1203  CGCCGGCG             C[GA]^CCGG[CT]G
SphI          435  GCATGC               GCATG^C
SspBI         419  TGTACA               T^GTACA
Tru9I        1814  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BfaI         1189  CTAG                 C^TAG
             1697  CTAG                
BspMI         836  ACCTGC               ACCTGC(4/8)
             2574  GCAGGT              
BsrBI          55  CCGCTC               CCGCTC(-3/-3)
             1773  GAGCGG              
BsrDI         859  CATTGC               GCAATG(2/0)
             1481  GCAATG              
BstYI         473  AGATCC               [GA]^GATC[CT]
             1277  AGATCC              
DraIII        754  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              841  CACCCGGTG           
HinfI        1690  GAGTC                G^A[ACGT]TC
             2378  GAGTC               
KspI          590  CCGCGG               CCGC^GG
             1364  CCGCGG              
MaeI         1189  CTAG                 C^TAG
             1697  CTAG                
MaeII         797  ACGT                 A^CGT
             1226  ACGT                
MaeIII        181  GTGAC                ^GT[ACGT]AC
             2271  GTCAC               
MflI          473  AGATCC               [GA]^GATC[CT]
             1277  AGATCC              
PleI         1690  GAGTC                GAGTC(4/5)
             2378  GAGTC               
SacII         590  CCGCGG               CCGC^GG
             1364  CCGCGG              
SfiI           15  GGCCGGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1746  GGCCTCCCGGGCC       
Tsp45I        181  GTGAC                ^GT[GC]AC
             2271  GTCAC               
XhoII         473  AGATCC               [GA]^GATC[CT]
             1277  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       Aor51HI     ApaI        AspHI       
AvaI        AvaII       BanI        BanII       BbeI        
BbsI        BbvI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssHII      BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DrdI        DsaI        EaeI        EagI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        KasI        
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        StuI        StyI        
TaqI        TseI        TspRI       TthHB8I     XmaCI       
XmaI