Restriction map for
pF1KSDA2013
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I AspI AviII
AvrII BamHI BbsI BbvCI BciVI
BclI BfrI BlnI BpuAI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI Eco47III Eco81I EcoO65I EcoRI
EcoRV FbaI FseI FspI HindIII
HpaI MamI MfeI MluI MroI
MslI MunI NcoI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 246 GGTACC G^GTACC
AhdI 593 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1266 GATCC GGATC(4/5)
Asp718I 246 GGTACC G^GTACC
AspEI 593 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1522 CACGTG CAC^GTG
BcgI 180 CGAGCCGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1957 CTAG C^TAG
BglII 1556 AGATCT A^GATCT
BsaAI 1522 CACGTG [CT]AC^GT[GA]
BsiEI 543 CGGCCG CG[GA][CT]^CG
BsrBI 337 GAGCGG CCGCTC(-3/-3)
BsrDI 1445 CATTGC GCAATG(2/0)
DraIII 778 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 543 CGGCCG C^GGCCG
Eam1105I 593 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 543 CGGCCG C^GGCCG
Eco52I 543 CGGCCG C^GGCCG
EcoNI 705 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1442 ATGCAT ATGCA^T
HincII 1173 GTCGAC GT[CT]^[GA]AC
HindII 1173 GTCGAC GT[CT]^[GA]AC
KpnI 246 GGTACC GGTAC^C
MaeI 1957 CTAG C^TAG
MseI 884 TTAA T^TAA
NheI 1956 GCTAGC G^CTAGC
NsiI 1442 ATGCAT ATGCA^T
PflMI 478 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1522 CACGTG CAC^GTG
PmlI 1522 CACGTG CAC^GTG
PshAI 35 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1173 GTCGAC G^TCGAC
SapI 1020 GCTCTTC GCTCTTC(1/4)
ScaI 1847 AGTACT AGT^ACT
SexAI 779 ACCTGGT A^CC[AT]GGT
Tru9I 884 TTAA T^TAA
Van91I 478 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 601 GTCTAC GT^[AC][GT]AC
1173 GTCGAC
AflIII 1521 ACACGT A^C[GA][CT]GT
1523 ACGTGT
Alw44I 775 GTGCAC G^TGCAC
865 GTGCAC
ApaLI 775 GTGCAC G^TGCAC
865 GTGCAC
BalI 278 TGGCCA TGG^CCA
1542 TGGCCA
BsaI 719 GAGACC GGTCTC(1/5)
1160 GAGACC
BssHII 116 GCGCGC G^CGCGC
395 GCGCGC
BstYI 1265 AGATCC [GA]^GATC[CT]
1556 AGATCT
KspI 159 CCGCGG CCGC^GG
533 CCGCGG
MaeIII 325 GTGAC ^GT[ACGT]AC
1639 GTGAC
MflI 1265 AGATCC [GA]^GATC[CT]
1556 AGATCT
MluNI 278 TGGCCA TGG^CCA
1542 TGGCCA
MscI 278 TGGCCA TGG^CCA
1542 TGGCCA
NspI 971 ACATGC [GA]CATG^[CT]
1103 ACATGC
SacI 919 GAGCTC GAGCT^C
961 GAGCTC
SacII 159 CCGCGG CCGC^GG
533 CCGCGG
SbfI 1350 CCTGCAGG CCTGCA^GG
1689 CCTGCAGG
SfiI 270 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1722 GGCCATCCTGGCC
Sse8387I 1350 CCTGCAGG CCTGCA^GG
1689 CCTGCAGG
Tsp45I 325 GTGAC ^GT[GC]AC
1639 GTGAC
XhoII 1265 AGATCC [GA]^GATC[CT]
1556 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TseI TspRI
TthHB8I XcmI XmaCI XmaI