Restriction map for
pF1KSDA1993
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII AccI AclI AcyI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspEI AspI AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BpuAI BsaBI BsaHI BsiWI BsmI
BspDI BspHI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII Hin1I HpaI KasI MamI
MfeI MluI MluNI MscI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 616 CGCG CG^CG
AccIII 551 TCCGGA T^CCGGA
AcsI 1484 AAATTC [GA]^AATT[CT]
Alw44I 363 GTGCAC G^TGCAC
AlwI 372 GATCC GGATC(4/5)
ApaLI 363 GTGCAC G^TGCAC
ApoI 1484 AAATTC [GA]^AATT[CT]
AviII 581 TGCGCA TGC^GCA
BlpI 945 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 945 GCTGAGC GC^T[ACGT]AGC
BsaAI 1420 TACGTG [CT]AC^GT[GA]
BsaWI 551 TCCGGA [AT]^CCGG[AT]
BseAI 551 TCCGGA T^CCGGA
BsiEI 735 CGACCG CG[GA][CT]^CG
BsmBI 559 GAGACG CGTCTC(1/5)
Bsp1407I 117 TGTACA T^GTACA
BspEI 551 TCCGGA T^CCGGA
BsrFI 737 ACCGGC [GA]^CCGG[CT]
BsrGI 117 TGTACA T^GTACA
BstAPI 154 GCAGTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 616 CGCG CG^CG
BstXI 485 CCAACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 945 GCTGAGC GC^T[ACGT]AGC
CfoI 582 GCGC GCG^C
Cfr10I 737 ACCGGC [GA]^CCGG[CT]
DraII 1016 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1489 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 615 CCGCGG C^C[GA][CT]GG
EarI 436 GAAGAG CTCTTC(1/4)
EcoNI 1020 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1016 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1329 CCAAGG C^C[AT][AT]GG
FspI 581 TGCGCA TGC^GCA
HgaI 1444 GACGC GACGC(5/10)
HhaI 582 GCGC GCG^C
HinP1I 582 GCGC G^CGC
HincII 412 GTTGAC GT[CT]^[GA]AC
HindII 412 GTTGAC GT[CT]^[GA]AC
Ksp632I 436 GAAGAG CTCTTC(1/4)
KspI 615 CCGCGG CCGC^GG
MaeII 1421 ACGT A^CGT
MroI 551 TCCGGA T^CCGGA
MvnI 616 CGCG CG^CG
NdeI 1192 CATATG CA^TATG
NspI 1181 ACATGC [GA]CATG^[CT]
SacII 615 CCGCGG CCGC^GG
SspBI 117 TGTACA T^GTACA
StyI 1329 CCAAGG C^C[AT][AT]GG
TaqI 453 TCGA T^CGA
TthHB8I 453 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 1015 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1493 CAGTGTCTG
AspHI 363 GTGCAC G[AT]GC[AT]^C
844 GTGCTC
BglII 497 AGATCT A^GATCT
1304 AGATCT
BpmI 847 CTCCAG CTGGAG(16/14)
1255 CTGGAG
BsaI 662 GAGACC GGTCTC(1/5)
677 GAGACC
BsgI 1000 CTGCAC GTGCAG(16/14)
1024 GTGCAG
BsiHKAI 363 GTGCAC G[AT]GC[AT]^C
844 GTGCTC
BspMI 998 ACCTGC ACCTGC(4/8)
1010 ACCTGC
HindIII 575 AAGCTT A^AGCTT
902 AAGCTT
MseI 199 TTAA T^TAA
233 TTAA
PvuII 295 CAGCTG CAG^CTG
744 CAGCTG
SbfI 90 CCTGCAGG CCTGCA^GG
1011 CCTGCAGG
SmaI 1060 CCCGGG CCC^GGG
1079 CCCGGG
Sse8387I 90 CCTGCAGG CCTGCA^GG
1011 CCTGCAGG
Tru9I 199 TTAA T^TAA
233 TTAA
Tsp509I 922 AATT ^AATT
1485 AATT
XmaCI 1060 CCCGGG C^CCGGG
1079 CCCGGG
XmaI 1060 CCCGGG C^CCGGG
1079 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
Acc65I AciI AfaI AluI Asp718I
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
KpnI MaeI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
TseI Tsp45I TspRI XhoII