Restriction map for
pF1KSDA1990
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbrPI BbvCI BclI BglI
BlnI BlpI Bpu1102I BsaAI BsiWI
BsmBI Bsp1407I BspDI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII
BstPI Bsu36I CelII ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NspV PacI
PflFI PflMI PmaCI PmeI PmlI
PshBI PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1846 GACGTC GACGT^C
AccI 2065 GTATAC GT^[AC][GT]AC
AccIII 2105 TCCGGA T^CCGGA
AcsI 869 GAATTT [GA]^AATT[CT]
AflII 1804 CTTAAG C^TTAAG
AflIII 1574 ACATGT A^C[GA][CT]GT
AlwNI 246 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 869 GAATTT [GA]^AATT[CT]
BcgI 1110 CGAGTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2335 CTAG C^TAG
BfrI 1804 CTTAAG C^TTAAG
BglII 614 AGATCT A^GATCT
BsaBI 2523 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2105 TCCGGA T^CCGGA
BsgI 1123 GTGCAG GTGCAG(16/14)
BsiEI 1389 CGACCG CG[GA][CT]^CG
BsmFI 1346 GGGAC GGGAC(10/14)
BspEI 2105 TCCGGA T^CCGGA
BspLU11I 1574 ACATGT A^CATGT
Bst1107I 2065 GTATAC GTA^TAC
BstAPI 586 GCAAGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2065 GTATAC GTA^TAC
DraII 1469 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1675 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1777 GACCAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 107 CCATGG C^C[GA][CT]GG
EcoO109I 1469 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 532 ATGCAT ATGCA^T
HincII 712 GTCAAC GT[CT]^[GA]AC
HindII 712 GTCAAC GT[CT]^[GA]AC
MaeI 2335 CTAG C^TAG
MamI 2523 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2105 TCCGGA T^CCGGA
MseI 1805 TTAA T^TAA
NcoI 107 CCATGG C^CATGG
NruI 2253 TCGCGA TCG^CGA
NsiI 532 ATGCAT ATGCA^T
PaeR7I 1886 CTCGAG C^TCGAG
PpuMI 1469 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2207 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1448 AGTACT AGT^ACT
SphI 1691 GCATGC GCATG^C
Tru9I 1805 TTAA T^TAA
XcmI 2303 CCATGTTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1886 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AclI 1231 AACGTT AA^CGTT
1253 AACGTT
AcyI 1846 GACGTC G[GA]^CG[CT]C
2257 GACGCC
AgeI 11 ACCGGT A^CCGGT
1357 ACCGGT
Alw44I 511 GTGCAC G^TGCAC
2449 GTGCAC
ApaLI 511 GTGCAC G^TGCAC
2449 GTGCAC
BalI 723 TGGCCA TGG^CCA
2088 TGGCCA
BamHI 350 GGATCC G^GATCC
1988 GGATCC
BanII 1262 GAGCCC G[GA]GC[CT]^C
2359 GAGCCC
BsaHI 1846 GACGTC G[GA]^CG[CT]C
2257 GACGCC
BsmI 697 GAATGC GAATGC(1/-1)
2109 GAATGC
BspHI 1052 TCATGA T^CATGA
1760 TCATGA
BspMI 1832 ACCTGC ACCTGC(4/8)
1782 GCAGGT
HaeII 2426 AGCGCC [GA]GCGC^[CT]
2594 AGCGCC
HgaI 2007 GACGC GACGC(5/10)
2257 GACGC
Hin1I 1846 GACGTC G[GA]^CG[CT]C
2257 GACGCC
MluNI 723 TGGCCA TGG^CCA
2088 TGGCCA
MscI 723 TGGCCA TGG^CCA
2088 TGGCCA
MslI 162 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1569 CATTGACATG
PinAI 11 ACCGGT A^CCGGT
1357 ACCGGT
Psp1406I 1231 AACGTT AA^CGTT
1253 AACGTT
PvuII 89 CAGCTG CAG^CTG
955 CAGCTG
RcaI 1052 TCATGA T^CATGA
1760 TCATGA
SapI 2476 GAAGAGC GCTCTTC(1/4)
2125 GCTCTTC
SexAI 244 ACCAGGT A^CC[AT]GGT
518 ACCTGGT
SmlI 1804 CTTAAG C^T[CT][GA]AG
1886 CTCGAG
TaqI 22 TCGA T^CGA
1887 TCGA
TthHB8I 22 TCGA T^CGA
1887 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BbsI BbvI
BciVI BcnI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp45I Tsp509I TspRI
XhoII