Restriction map for
pF1KSDA1971
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccI AflIII AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI AspI AviII BamHI BbsI
BciVI BpuAI BsaI BsgI BsiWI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstZ17I ClaI
DrdI Eam1105I EcoRI EcoT22I FspI
HincII HindII HpaI KpnI MfeI
MluI MslI MunI NdeI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmeI PvuI SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AclI 655 AACGTT AA^CGTT
AcyI 484 GGCGCC G[GA]^CG[CT]C
AflII 2148 CTTAAG C^TTAAG
AocI 205 CCTGAGG CC^T[ACGT]AGG
Aor51HI 305 AGCGCT AGC^GCT
AseI 648 ATTAAT AT^TAAT
AsnI 648 ATTAAT AT^TAAT
AvrII 804 CCTAGG C^CTAGG
BalI 2412 TGGCCA TGG^CCA
BbeI 484 GGCGCC GGCGC^C
BbrPI 2090 CACGTG CAC^GTG
BbvCI 514 GCTGAGG CCTCAGC(-5/-2)
BcgI 2070 CGAATCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1875 TGATCA T^GATCA
BfrI 2148 CTTAAG C^TTAAG
BglII 1110 AGATCT A^GATCT
BlnI 804 CCTAGG C^CTAGG
BlpI 2080 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2080 GCTGAGC GC^T[ACGT]AGC
BsaAI 2090 CACGTG [CT]AC^GT[GA]
BsaBI 1622 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 484 GGCGCC G[GA]^CG[CT]C
BsmFI 407 GTCCC GGGAC(10/14)
BsmI 935 GAATGC GAATGC(1/-1)
BspHI 1049 TCATGA T^CATGA
BsrBI 559 GAGCGG CCGCTC(-3/-3)
BstAPI 75 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 693 GGTTACC G^GT[ACGT]ACC
BstPI 693 GGTTACC G^GT[ACGT]ACC
Bsu36I 205 CCTGAGG CC^T[ACGT]AGG
CelII 2080 GCTGAGC GC^T[ACGT]AGC
CpoI 41 CGGTCCG CG^G[AT]CCG
DraIII 1741 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 305 AGCGCT AGC^GCT
Eco81I 205 CCTGAGG CC^T[ACGT]AGG
EcoNI 2144 CCTCCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 693 GGTTACC G^GT[ACGT]ACC
EcoRV 889 GATATC GAT^ATC
FbaI 1875 TGATCA T^GATCA
FseI 234 GGCCGGCC GGCCGG^CC
Hin1I 484 GGCGCC G[GA]^CG[CT]C
HindIII 769 AAGCTT A^AGCTT
KasI 484 GGCGCC G^GCGCC
MamI 1622 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2412 TGGCCA TGG^CCA
MscI 2412 TGGCCA TGG^CCA
NarI 484 GGCGCC GG^CGCC
NcoI 638 CCATGG C^CATGG
NheI 1026 GCTAGC G^CTAGC
PaeR7I 1031 CTCGAG C^TCGAG
PmaCI 2090 CACGTG CAC^GTG
PmlI 2090 CACGTG CAC^GTG
PshAI 802 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 648 ATTAAT AT^TAAT
Psp1406I 655 AACGTT AA^CGTT
RcaI 1049 TCATGA T^CATGA
RsrII 41 CGGTCCG CG^G[AT]CCG
SacI 2162 GAGCTC GAGCT^C
ScaI 719 AGTACT AGT^ACT
SfoI 484 GGCGCC GGC^GCC
XhoI 1031 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 1445 TCCGGA T^CCGGA
2171 TCCGGA
AfaI 143 GTAC GT^AC
720 GTAC
ApaI 173 GGGCCC GGGCC^C
184 GGGCCC
Asp700I 1009 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1108 GAAGATCTTC
BglI 23 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
66 GCCCGAGAGGC
BsaWI 1445 TCCGGA [AT]^CCGG[AT]
2171 TCCGGA
BseAI 1445 TCCGGA T^CCGGA
2171 TCCGGA
BsmBI 216 CGTCTC CGTCTC(1/5)
876 CGTCTC
BspEI 1445 TCCGGA T^CCGGA
2171 TCCGGA
BspMI 1697 ACCTGC ACCTGC(4/8)
2076 ACCTGC
BstXI 632 CCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2415 CCAGTGGGATGG
DraI 1071 TTTAAA TTT^AAA
1633 TTTAAA
MroI 1445 TCCGGA T^CCGGA
2171 TCCGGA
NaeI 235 GCCGGC GCC^GGC
2187 GCCGGC
NgoMIV 235 GCCGGC G^CCGGC
2187 GCCGGC
PflMI 684 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1395 CCAGCAGCTGG
PstI 424 CTGCAG CTGCA^G
1348 CTGCAG
RsaI 143 GTAC GT^AC
720 GTAC
SfcI 424 CTGCAG C^T[GA][CT]AG
1348 CTGCAG
TaqI 584 TCGA T^CGA
1032 TCGA
TfiI 2071 GAATC G^A[AT]TC
2360 GAATC
TthHB8I 584 TCGA T^CGA
1032 TCGA
Van91I 684 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1395 CCAGCAGCTGG
XmnI 1009 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1108 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
SacII Sau3AI Sau96I ScrFI SfaNI
SmlI StyI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII