Restriction map for
pF1KSDA1960
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BclI BfrI
BglII BlnI BpuAI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 465 GACGTC GACGT^C
AgeI 2343 ACCGGT A^CCGGT
AhdI 862 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2086 CCTCAGG CC^T[ACGT]AGG
Aor51HI 916 AGCGCT AGC^GCT
AspEI 862 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 2042 GGCGCC G^G[CT][GA]CC
BbeI 2042 GGCGCC GGCGC^C
BciVI 1065 GTATCC GTATCC(6/5)
BlpI 564 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 564 GCTCAGC GC^T[ACGT]AGC
BsaWI 2343 ACCGGT [AT]^CCGG[AT]
BsgI 1922 CTGCAC GTGCAG(16/14)
BsmI 721 GAATGC GAATGC(1/-1)
BspMI 1123 ACCTGC ACCTGC(4/8)
BssSI 811 CACGAG CACGAG(-5/-1)
BstYI 1656 AGATCC [GA]^GATC[CT]
Bsu36I 2086 CCTCAGG CC^T[ACGT]AGG
CelII 564 GCTCAGC GC^T[ACGT]AGC
DraIII 1885 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 862 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 916 AGCGCT AGC^GCT
Eco81I 2086 CCTCAGG CC^T[ACGT]AGG
KasI 2042 GGCGCC G^GCGCC
MflI 1656 AGATCC [GA]^GATC[CT]
MslI 2027 CACAAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2042 GGCGCC GG^CGCC
PinAI 2343 ACCGGT A^CCGGT
SacI 484 GAGCTC GAGCT^C
ScaI 1795 AGTACT AGT^ACT
SfoI 2042 GGCGCC GGC^GCC
TfiI 1728 GATTC G^A[AT]TC
XhoII 1656 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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BsaAI 184 TACGTG [CT]AC^GT[GA]
2293 CACGTA
BsaI 1120 GAGACC GGTCTC(1/5)
1113 GGTCTC
BsrDI 1535 GCAATG GCAATG(2/0)
1753 GCAATG
EagI 356 CGGCCG C^GGCCG
627 CGGCCG
EarI 2543 CTCTTC CTCTTC(1/4)
1174 GAAGAG
EclXI 356 CGGCCG C^GGCCG
627 CGGCCG
Eco52I 356 CGGCCG C^GGCCG
627 CGGCCG
Ksp632I 2543 CTCTTC CTCTTC(1/4)
1174 GAAGAG
PaeR7I 408 CTCGAG C^TCGAG
1624 CTCGAG
PvuII 1478 CAGCTG CAG^CTG
1511 CAGCTG
SmlI 408 CTCGAG C^T[CT][GA]AG
1624 CTCGAG
TaqI 409 TCGA T^CGA
1625 TCGA
Tsp45I 1490 GTGAC ^GT[GC]AC
1670 GTGAC
TthHB8I 409 TCGA T^CGA
1625 TCGA
XcmI 47 CCACTAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1404 CCACACCCCCACTGG
XhoI 408 CTCGAG C^TCGAG
1624 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SmaI StuI
StyI TseI TspRI XmaCI XmaI