Restriction map for
pF1KSDA1917
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AvaII AvrII
BbeI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 721 GTAGAC GT^[AC][GT]AC
AccII 27 CGCG CG^CG
AccIII 85 TCCGGA T^CCGGA
AcyI 1491 GACGCC G[GA]^CG[CT]C
AspHI 1899 GTGCTC G[AT]GC[AT]^C
AvaI 383 CTCGGG C^[CT]CG[GA]G
AviII 1822 TGCGCA TGC^GCA
BamHI 534 GGATCC G^GATCC
BanI 1921 GGTGCC G^G[CT][GA]CC
BbrPI 1159 CACGTG CAC^GTG
BbvCI 301 GCTGAGG CCTCAGC(-5/-2)
BglII 749 AGATCT A^GATCT
BsaAI 1159 CACGTG [CT]AC^GT[GA]
BsaHI 1491 GACGCC G[GA]^CG[CT]C
BsaI 1386 GAGACC GGTCTC(1/5)
BsaWI 85 TCCGGA [AT]^CCGG[AT]
BseAI 85 TCCGGA T^CCGGA
BsiHKAI 1899 GTGCTC G[AT]GC[AT]^C
BsoBI 383 CTCGGG C^[CT]CG[GA]G
BspEI 85 TCCGGA T^CCGGA
BspMI 1217 ACCTGC ACCTGC(4/8)
BsrDI 640 CATTGC GCAATG(2/0)
BstEII 151 GGTTACC G^GT[ACGT]ACC
BstPI 151 GGTTACC G^GT[ACGT]ACC
BstUI 27 CGCG CG^CG
BstXI 1982 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 526 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 738 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 151 GGTTACC G^GT[ACGT]ACC
EcoT14I 1562 CCATGG C^C[AT][AT]GG
FspI 1822 TGCGCA TGC^GCA
HgaI 1491 GACGC GACGC(5/10)
Hin1I 1491 GACGCC G[GA]^CG[CT]C
MroI 85 TCCGGA T^CCGGA
MseI 1019 TTAA T^TAA
MvnI 27 CGCG CG^CG
NcoI 1562 CCATGG C^CATGG
NdeI 1199 CATATG CA^TATG
NspI 603 ACATGC [GA]CATG^[CT]
PflMI 525 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1159 CACGTG CAC^GTG
PmlI 1159 CACGTG CAC^GTG
PstI 1356 CTGCAG CTGCA^G
PvuII 1742 CAGCTG CAG^CTG
SexAI 1917 ACCTGGT A^CC[AT]GGT
SfcI 1356 CTGCAG C^T[GA][CT]AG
SgrAI 1966 CGCCGGCG C[GA]^CCGG[CT]G
SspI 1075 AATATT AAT^ATT
StyI 1562 CCATGG C^C[AT][AT]GG
Tru9I 1019 TTAA T^TAA
Van91I 525 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1532 CCAGCAGCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AspI 724 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1588 GACACCGTC
BbsI 243 GAAGAC GAAGAC(2/6)
799 GAAGAC
BcnI 852 CCGGG CC^[GC]GG
957 CCGGG
BfaI 595 CTAG C^TAG
1831 CTAG
BglI 1669 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1924 GCCTGGCAGGC
BpuAI 243 GAAGAC GAAGAC(2/6)
799 GAAGAC
BsgI 481 GTGCAG GTGCAG(16/14)
902 GTGCAG
BsrFI 17 GCCGGC [GA]^CCGG[CT]
1967 GCCGGC
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
1967 GCCGGC
DrdI 1491 GACGCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1939 GACAATGCCGTC
FauI 75 CCCGC CCCGC(4/6)
28 GCGGG
HaeII 1964 AGCGCC [GA]GCGC^[CT]
1970 GGCGCT
MaeI 595 CTAG C^TAG
1831 CTAG
MaeII 1160 ACGT A^CGT
1819 ACGT
MspA1I 1706 CAGCGG C[AC]G^C[GT]G
1742 CAGCTG
NaeI 17 GCCGGC GCC^GGC
1967 GCCGGC
NciI 852 CCGGG CC^[GC]GG
957 CCGGG
NgoMIV 17 GCCGGC G^CCGGC
1967 GCCGGC
PflFI 724 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1588 GACACCGTC
SapI 312 GAAGAGC GCTCTTC(1/4)
1054 GAAGAGC
Tsp45I 1423 GTGAC ^GT[GC]AC
1844 GTGAC
Tth111I 724 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1588 GACACCGTC
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AhdI AluI AlwI
ApaI AspEI BalI BanII BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI Tsp509I TspRI XhoII