Restriction map for
pF1KSDA1916
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BbrPI BbvCI BglI BglII
BlnI BlpI Bpu1102I BsaBI BseRI
BsiEI BsiHKAI BsiWI Bsp1407I BspLU11I
BsrDI BsrFI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I CpoI DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoT22I FseI FspI
HgaI HindIII HpaI KpnI MamI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 797 TCCGGA T^CCGGA
AflII 1626 CTTAAG C^TTAAG
AlwNI 1093 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 27 AGCGCT AGC^GCT
BanII 150 GGGCTC G[GA]GC[CT]^C
BbsI 454 GTCTTC GAAGAC(2/6)
BcgI 1521 GCAGGCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 699 GGATAC GTATCC(6/5)
BclI 858 TGATCA T^GATCA
BfaI 484 CTAG C^TAG
BfrI 1626 CTTAAG C^TTAAG
BpuAI 454 GTCTTC GAAGAC(2/6)
BsaAI 727 TACGTG [CT]AC^GT[GA]
BsaI 957 GGTCTC GGTCTC(1/5)
BsaWI 797 TCCGGA [AT]^CCGG[AT]
BseAI 797 TCCGGA T^CCGGA
BsmBI 1009 GAGACG CGTCTC(1/5)
BspEI 797 TCCGGA T^CCGGA
BspHI 1334 TCATGA T^CATGA
BspMI 84 GCAGGT ACCTGC(4/8)
BsrBI 1173 CCGCTC CCGCTC(-3/-3)
BssSI 1102 CACGAG CACGAG(-5/-1)
BstYI 1364 AGATCC [GA]^GATC[CT]
DraI 1414 TTTAAA TTT^AAA
DraII 1181 AGGTCCC [GA]G^G[ACGT]CC[CT]
Eco47III 27 AGCGCT AGC^GCT
EcoO109I 1181 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 276 GAATTC G^AATTC
EcoRV 1138 GATATC GAT^ATC
FbaI 858 TGATCA T^GATCA
HincII 1621 GTTGAC GT[CT]^[GA]AC
HindII 1621 GTTGAC GT[CT]^[GA]AC
KspI 73 CCGCGG CCGC^GG
MaeI 484 CTAG C^TAG
MflI 1364 AGATCC [GA]^GATC[CT]
MroI 797 TCCGGA T^CCGGA
NcoI 1230 CCATGG C^CATGG
PpuMI 1181 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1334 TCATGA T^CATGA
SacII 73 CCGCGG CCGC^GG
SmlI 1626 CTTAAG C^T[CT][GA]AG
TfiI 1079 GATTC G^A[AT]TC
XcmI 882 CCAGCTCTTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1364 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 55 GGCGCC G[GA]^CG[CT]C
1524 GGCGCC
AlwI 1365 GATCC GGATC(4/5)
170 GGATC
Asp700I 236 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1269 GAAGAGCTTC
BalI 419 TGGCCA TGG^CCA
731 TGGCCA
BbeI 55 GGCGCC GGCGC^C
1524 GGCGCC
BmyI 150 GGGCTC G[AGT]GC[ACT]^C
163 GTGCCC
BpmI 1195 CTCCAG CTGGAG(16/14)
769 CTGGAG
BsaHI 55 GGCGCC G[GA]^CG[CT]C
1524 GGCGCC
BsgI 1099 CTGCAC GTGCAG(16/14)
1519 GTGCAG
BsmI 640 GAATGC GAATGC(1/-1)
1280 GAATGC
Bsp1286I 150 GGGCTC G[AGT]GC[ACT]^C
163 GTGCCC
BspDI 853 ATCGAT AT^CGAT
1141 ATCGAT
BssHII 80 GCGCGC G^CGCGC
100 GCGCGC
ClaI 853 ATCGAT AT^CGAT
1141 ATCGAT
DrdI 949 GACATAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1324 GACTCCCGGGTC
DsaI 73 CCGCGG C^C[GA][CT]GG
1230 CCATGG
EaeI 419 TGGCCA [CT]^GGCC[GA]
731 TGGCCA
FauI 109 CCCGC CCCGC(4/6)
1310 CCCGC
Hin1I 55 GGCGCC G[GA]^CG[CT]C
1524 GGCGCC
KasI 55 GGCGCC G^GCGCC
1524 GGCGCC
MluNI 419 TGGCCA TGG^CCA
731 TGGCCA
MscI 419 TGGCCA TGG^CCA
731 TGGCCA
MslI 141 CATCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
783 CATTGAAATG
NarI 55 GGCGCC GG^CGCC
1524 GGCGCC
PstI 259 CTGCAG CTGCA^G
1399 CTGCAG
SfoI 55 GGCGCC GGC^GCC
1524 GGCGCC
SmaI 1328 CCCGGG CCC^GGG
1382 CCCGGG
XmaCI 1328 CCCGGG C^CCGGG
1382 CCCGGG
XmaI 1328 CCCGGG C^CCGGG
1382 CCCGGG
XmnI 236 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1269 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AvaI AvaII BanI BbvI
BcnI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I