Restriction map for
pF1KSDA1911
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlnI BsaAI BsaHI
BsaJI BseAI BseRI BsiEI BsiHKAI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstXI CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRV
EcoT14I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeII MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflIII 519 ACATGT A^C[GA][CT]GT
AocI 2370 CCTAAGG CC^T[ACGT]AGG
BalI 1454 TGGCCA TGG^CCA
BanII 1433 GGGCTC G[GA]GC[CT]^C
BlpI 1148 GCTTAGC GC^T[ACGT]AGC
BmyI 1433 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 1148 GCTTAGC GC^T[ACGT]AGC
BsaBI 565 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1027 GAGACC GGTCTC(1/5)
BsaWI 356 ACCGGA [AT]^CCGG[AT]
BsgI 1204 GTGCAG GTGCAG(16/14)
BsmBI 701 GAGACG CGTCTC(1/5)
Bsp1286I 1433 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 519 ACATGT A^CATGT
BsrDI 1080 GCAATG GCAATG(2/0)
Bsu36I 2370 CCTAAGG CC^T[ACGT]AGG
CelII 1148 GCTTAGC GC^T[ACGT]AGC
EaeI 1454 TGGCCA [CT]^GGCC[GA]
Eco81I 2370 CCTAAGG CC^T[ACGT]AGG
EcoT22I 1285 ATGCAT ATGCA^T
FauI 2300 GCGGG CCCGC(4/6)
HaeIII 1455 GGCC GG^CC
HapII 357 CCGG C^CGG
HgaI 2334 GCGTC GACGC(5/10)
HpaII 357 CCGG C^CGG
MamI 565 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1454 TGGCCA TGG^CCA
MscI 1454 TGGCCA TGG^CCA
MslI 2096 CACTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 357 CCGG C^CGG
MwoI 843 GCCATCAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 1285 ATGCAT ATGCA^T
NspI 519 ACATGT [GA]CATG^[CT]
PflMI 1539 CCAATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI 2190 CTACAG C^T[GA][CT]AG
SmlI 1226 CTCAAG C^T[CT][GA]AG
Van91I 1539 CCAATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1127 GTATAC GT^[AC][GT]AC
1875 GTATAC
AciI 2170 GCGG CCGC(-3/-1)
2300 GCGG
Asp700I 1718 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1966 GAAAGAATTC
AvaII 118 GGTCC G^G[AT]CC
765 GGTCC
BbsI 687 GAAGAC GAAGAC(2/6)
2017 GAAGAC
BcgI 710 CGAAACCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1080 GCAATGTAATCG
BpmI 910 CTCCAG CTGGAG(16/14)
874 CTGGAG
BpuAI 687 GAAGAC GAAGAC(2/6)
2017 GAAGAC
BsmFI 1318 GGGAC GGGAC(10/14)
766 GTCCC
BsmI 1868 GAATGC GAATGC(1/-1)
2365 GAATGC
BssKI 1642 CCAGG ^CC[ACGT]GG
2264 CCTGG
Bst1107I 1127 GTATAC GTA^TAC
1875 GTATAC
BstF5I 1291 CATCC GGATG(2/0)
1074 GGATG
BstNI 1642 CCAGG CC^[AT]GG
2264 CCTGG
BstYI 667 GGATCT [GA]^GATC[CT]
2427 AGATCC
BstZ17I 1127 GTATAC GTA^TAC
1875 GTATAC
Cfr13I 118 GGTCC G^G[ACGT]CC
765 GGTCC
EcoNI 1237 CCTAAATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2034 CCTGAAAAAGG
EcoRII 1642 CCAGG ^CC[AT]GG
2264 CCTGG
FokI 1291 CATCC GGATG(9/13)
1074 GGATG
HincII 2044 GTTGAC GT[CT]^[GA]AC
2065 GTTGAC
HindII 2044 GTTGAC GT[CT]^[GA]AC
2065 GTTGAC
MflI 667 GGATCT [GA]^GATC[CT]
2427 AGATCC
MvaI 1642 CCAGG CC^[AT]GG
2264 CCTGG
Sau96I 118 GGTCC G^G[ACGT]CC
765 GGTCC
ScrFI 1642 CCAGG CC^[ACGT]GG
2264 CCTGG
TaqI 1594 TCGA T^CGA
2310 TCGA
TthHB8I 1594 TCGA T^CGA
2310 TCGA
XhoII 667 GGATCT [GA]^GATC[CT]
2427 AGATCC
XmnI 1718 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1966 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AseI AsnI BbvI BfaI
BsiYI BslI BsmAI BsrI Cac8I
DdeI DpnII EarI EcoRI Fnu4HI
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII NlaIV PleI PshBI
RsaI Sau3AI ScaI SfaNI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI