Restriction map for
pF1KSDA1899
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BbrPI BciVI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BseAI BsiEI BsiWI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FseI
FspI HindIII HpaI KpnI KspI
MluI MroI NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 556 GTAGAC GT^[AC][GT]AC
AcyI 1462 GGCGCC G[GA]^CG[CT]C
AgeI 2816 ACCGGT A^CCGGT
Alw44I 1441 GTGCAC G^TGCAC
AlwNI 1699 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1441 GTGCAC G^TGCAC
Asp700I 2172 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 960 TGGCCA TGG^CCA
BbeI 1462 GGCGCC GGCGC^C
BbsI 1808 GTCTTC GAAGAC(2/6)
BbvCI 2400 CCTCAGC CCTCAGC(-5/-2)
BclI 516 TGATCA T^GATCA
BpuAI 1808 GTCTTC GAAGAC(2/6)
BsaBI 2764 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1462 GGCGCC G[GA]^CG[CT]C
BsaWI 2816 ACCGGT [AT]^CCGG[AT]
BseRI 2192 GAGGAG GAGGAG(10/8)
BsgI 1234 CTGCAC GTGCAG(16/14)
BsmBI 1371 CGTCTC CGTCTC(1/5)
BsrDI 1890 CATTGC GCAATG(2/0)
BssSI 1981 CACGAG CACGAG(-5/-1)
BstAPI 1730 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1237 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 960 TGGCCA [CT]^GGCC[GA]
EcoRI 548 GAATTC G^AATTC
EcoT22I 2671 ATGCAT ATGCA^T
FauI 57 GCGGG CCCGC(4/6)
FbaI 516 TGATCA T^GATCA
HaeII 1462 GGCGCC [GA]GCGC^[CT]
HgaI 34 GACGC GACGC(5/10)
Hin1I 1462 GGCGCC G[GA]^CG[CT]C
KasI 1462 GGCGCC G^GCGCC
MamI 2764 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2773 CAATTG C^AATTG
MluNI 960 TGGCCA TGG^CCA
MscI 960 TGGCCA TGG^CCA
MspA1I 1699 CAGCTG C[AC]G^C[GT]G
MunI 2773 CAATTG C^AATTG
NaeI 79 GCCGGC GCC^GGC
NarI 1462 GGCGCC GG^CGCC
NgoMIV 79 GCCGGC G^CCGGC
NsiI 2671 ATGCAT ATGCA^T
PinAI 2816 ACCGGT A^CCGGT
PpuMI 2411 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1699 CAGCTG CAG^CTG
SacI 61 GAGCTC GAGCT^C
SfoI 1462 GGCGCC GGC^GCC
SphI 1022 GCATGC GCATG^C
XbaI 2097 TCTAGA T^CTAGA
XmnI 2172 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AflII 528 CTTAAG C^TTAAG
2655 CTTAAG
AflIII 1535 ACACGT A^C[GA][CT]GT
2216 ACACGT
ApaI 1097 GGGCCC GGGCC^C
1618 GGGCCC
BamHI 600 GGATCC G^GATCC
3013 GGATCC
BcnI 69 CCGGG CC^[GC]GG
1293 CCGGG
BfrI 528 CTTAAG C^TTAAG
2655 CTTAAG
BsrFI 79 GCCGGC [GA]^CCGG[CT]
2816 ACCGGT
Cfr10I 79 GCCGGC [GA]^CCGG[CT]
2816 ACCGGT
DraI 2959 TTTAAA TTT^AAA
2991 TTTAAA
EarI 252 GAAGAG CTCTTC(1/4)
505 GAAGAG
EcoNI 1317 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1622 CCTCCAGCAGG
Ksp632I 252 GAAGAG CTCTTC(1/4)
505 GAAGAG
MslI 1638 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2862 CATGAAGGTG
NciI 69 CCGGG CC^[GC]GG
1293 CCGGG
NcoI 1640 CCATGG C^CATGG
2416 CCATGG
NdeI 956 CATATG CA^TATG
2971 CATATG
PshAI 460 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2815 GACCGGTGTC
SspI 895 AATATT AAT^ATT
1109 AATATT
StuI 2571 AGGCCT AGG^CCT
2910 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BanII BbvI BcgI BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspHI
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII