Restriction map for
pF1KSDA1896
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaHI BsiWI BspDI BspLU11I BsrBI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT22I FbaI FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MfeI MluI MunI
NarI NcoI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 1338 TCCGGA T^CCGGA
ApaI 456 GGGCCC GGGCC^C
Asp700I 418 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 550 CCCGAG C^[CT]CG[GA]G
BamHI 1070 GGATCC G^GATCC
BanII 456 GGGCCC G[GA]GC[CT]^C
BciVI 691 GTATCC GTATCC(6/5)
BsaAI 1456 TACGTG [CT]AC^GT[GA]
BsaBI 489 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 113 GAGACC GGTCTC(1/5)
BsaWI 1338 TCCGGA [AT]^CCGG[AT]
BseAI 1338 TCCGGA T^CCGGA
BsiEI 75 CGATCG CG[GA][CT]^CG
BsmBI 1168 GAGACG CGTCTC(1/5)
BsoBI 550 CCCGAG C^[CT]CG[GA]G
BspEI 1338 TCCGGA T^CCGGA
BsrDI 830 GCAATG GCAATG(2/0)
BsrFI 1144 GCCGGC [GA]^CCGG[CT]
BssSI 937 CACGAG CACGAG(-5/-1)
BstAPI 372 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1344 GGTGACC G^GT[ACGT]ACC
BstPI 1344 GGTGACC G^GT[ACGT]ACC
BstYI 1070 GGATCC [GA]^GATC[CT]
Cfr10I 1144 GCCGGC [GA]^CCGG[CT]
CpoI 1378 CGGACCG CG^G[AT]CCG
EarI 1360 CTCTTC CTCTTC(1/4)
EcoO65I 1344 GGTGACC G^GT[ACGT]ACC
EcoRV 399 GATATC GAT^ATC
EcoT14I 959 CCTTGG C^C[AT][AT]GG
HindIII 126 AAGCTT A^AGCTT
Ksp632I 1360 CTCTTC CTCTTC(1/4)
MamI 489 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1070 GGATCC [GA]^GATC[CT]
MroI 1338 TCCGGA T^CCGGA
MseI 320 TTAA T^TAA
NaeI 1144 GCCGGC GCC^GGC
NgoMIV 1144 GCCGGC G^CCGGC
NheI 28 GCTAGC G^CTAGC
NspI 1121 ACATGC [GA]CATG^[CT]
PvuI 75 CGATCG CGAT^CG
RsrII 1378 CGGACCG CG^G[AT]CCG
ScaI 1040 AGTACT AGT^ACT
SexAI 662 ACCTGGT A^CC[AT]GGT
StyI 959 CCTTGG C^C[AT][AT]GG
Tru9I 320 TTAA T^TAA
XhoII 1070 GGATCC [GA]^GATC[CT]
XmnI 418 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 1162 GTCTAC GT^[AC][GT]AC
1462 GTCTAC
AlwI 1071 GATCC GGATC(4/5)
1070 GGATC
AspHI 730 GTGCTC G[AT]GC[AT]^C
1231 GTGCTC
BbsI 1008 GAAGAC GAAGAC(2/6)
1029 GAAGAC
BbvCI 154 GCTGAGG CCTCAGC(-5/-2)
306 GCTGAGG
BpuAI 1008 GAAGAC GAAGAC(2/6)
1029 GAAGAC
BseRI 526 CTCCTC GAGGAG(10/8)
631 GAGGAG
BsgI 702 CTGCAC GTGCAG(16/14)
1492 GTGCAG
BsiHKAI 730 GTGCTC G[AT]GC[AT]^C
1231 GTGCTC
Bsp1407I 225 TGTACA T^GTACA
1400 TGTACA
BspHI 294 TCATGA T^CATGA
1424 TCATGA
BsrGI 225 TGTACA T^GTACA
1400 TGTACA
BstXI 179 CCATTTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1118 CCAACATGCTGG
DraIII 532 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
661 CACCTGGTG
DrdI 385 GACTTGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1153 GACCTTGCAGTC
MaeII 842 ACGT A^CGT
1457 ACGT
MslI 465 CACCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
786 CACTCAGATG
MspA1I 1270 CAGCTG C[AC]G^C[GT]G
1439 CAGCTG
PflMI 494 CCATCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
531 CCACCCCGTGG
PleI 924 GACTC GAGTC(4/5)
392 GAGTC
PstI 200 CTGCAG CTGCA^G
1189 CTGCAG
PvuII 1270 CAGCTG CAG^CTG
1439 CAGCTG
RcaI 294 TCATGA T^CATGA
1424 TCATGA
SfcI 200 CTGCAG C^T[GA][CT]AG
1189 CTGCAG
SspBI 225 TGTACA T^GTACA
1400 TGTACA
TspRI 626 CAGTG CA[GC]TG[ACGT][ACGT]^
1202 CAGTG
Van91I 494 CCATCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
531 CCACCCCGTGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI ApoI AvaII BalI BanI
BbvI BcnI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MluNI MnlI MscI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI TfiI
TseI Tsp45I Tsp509I TthHB8I XcmI