Restriction map for
pF1KSDA1892
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstUI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 139 GTATAC GT^[AC][GT]AC
AlwNI 593 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 69 GGGCCC GGGCC^C
AvaI 191 CTCGAG C^[CT]CG[GA]G
BbrPI 605 CACGTG CAC^GTG
BbsI 736 GAAGAC GAAGAC(2/6)
BciVI 425 GTATCC GTATCC(6/5)
BpuAI 736 GAAGAC GAAGAC(2/6)
BsaAI 605 CACGTG [CT]AC^GT[GA]
BsaWI 1028 TCCGGT [AT]^CCGG[AT]
BsoBI 191 CTCGAG C^[CT]CG[GA]G
BspHI 1206 TCATGA T^CATGA
Bst1107I 139 GTATAC GTA^TAC
BstAPI 317 GCACAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 139 GTATAC GTA^TAC
CfoI 34 GCGC GCG^C
DraIII 670 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
HaeII 33 AGCGCC [GA]GCGC^[CT]
HgaI 61 GACGC GACGC(5/10)
HhaI 34 GCGC GCG^C
HinP1I 34 GCGC G^CGC
NcoI 1332 CCATGG C^CATGG
NheI 462 GCTAGC G^CTAGC
PaeR7I 191 CTCGAG C^TCGAG
PflMI 566 CCATCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 605 CACGTG CAC^GTG
PmlI 605 CACGTG CAC^GTG
RcaI 1206 TCATGA T^CATGA
SacI 1103 GAGCTC GAGCT^C
SfcI 169 CTACAG C^T[GA][CT]AG
SmlI 191 CTCGAG C^T[CT][GA]AG
StuI 713 AGGCCT AGG^CCT
Tsp509I 1225 AATT ^AATT
Van91I 566 CCATCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 451 TCTAGA T^CTAGA
XcmI 411 CCAGCAGGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 191 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AspHI 1048 GAGCAC G[AT]GC[AT]^C
1103 GAGCTC
BamHI 519 GGATCC G^GATCC
966 GGATCC
BbvI 211 GCAGC GCAGC(8/12)
975 GCAGC
BseRI 862 CTCCTC GAGGAG(10/8)
473 GAGGAG
BsiHKAI 1048 GAGCAC G[AT]GC[AT]^C
1103 GAGCTC
DsaI 970 CCACGG C^C[GA][CT]GG
1332 CCATGG
EarI 126 GAAGAG CTCTTC(1/4)
1120 GAAGAG
EcoT14I 255 CCTTGG C^C[AT][AT]GG
1332 CCATGG
FauI 37 CCCGC CCCGC(4/6)
72 CCCGC
Fnu4HI 211 GCAGC GC^[ACGT]GC
975 GCAGC
ItaI 211 GCAGC GC^[ACGT]GC
975 GCAGC
Ksp632I 126 GAAGAG CTCTTC(1/4)
1120 GAAGAG
MaeII 606 ACGT A^CGT
989 ACGT
MseI 266 TTAA T^TAA
718 TTAA
PleI 623 GACTC GAGTC(4/5)
1279 GACTC
SfaNI 661 GATGC GCATC(5/9)
280 GCATC
StyI 255 CCTTGG C^C[AT][AT]GG
1332 CCATGG
Tru9I 266 TTAA T^TAA
718 TTAA
TseI 211 GCAGC G^C[AT]GC
975 GCAGC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BcnI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FokI HaeIII HapII
HinfI HpaII HphI MaeI MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI Sau3AI Sau96I
ScrFI TaqI TfiI Tsp45I TspRI
TthHB8I XhoII