Restriction map for
pF1KSDA1874
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstPI BstUI Bsu36I
Cac8I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccI 577 GTATAC GT^[AC][GT]AC
BalI 149 TGGCCA TGG^CCA
BamHI 186 GGATCC G^GATCC
BsaI 969 GAGACC GGTCTC(1/5)
BsaWI 1465 ACCGGA [AT]^CCGG[AT]
BseRI 169 GAGGAG GAGGAG(10/8)
BspMI 814 ACCTGC ACCTGC(4/8)
Bst1107I 577 GTATAC GTA^TAC
BstAPI 899 GCAGTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 166 CCAGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 577 GTATAC GTA^TAC
CfoI 105 GCGC GCG^C
DsaI 152 CCATGG C^C[GA][CT]GG
EcoNI 25 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 152 CCATGG C^C[AT][AT]GG
HhaI 105 GCGC GCG^C
HinP1I 105 GCGC G^CGC
MaeII 959 ACGT A^CGT
MaeIII 133 GTGAC ^GT[ACGT]AC
MluNI 149 TGGCCA TGG^CCA
MscI 149 TGGCCA TGG^CCA
NcoI 152 CCATGG C^CATGG
NdeI 1226 CATATG CA^TATG
PflMI 267 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI 219 CTATAG C^T[GA][CT]AG
SmlI 301 CTTGAG C^T[CT][GA]AG
StyI 152 CCATGG C^C[AT][AT]GG
Tsp45I 133 GTGAC ^GT[GC]AC
Van91I 267 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 1013 GTAC GT^AC
1395 GTAC
BanII 35 GAGCTC G[GA]GC[CT]^C
1347 GAGCTC
BbsI 619 GAAGAC GAAGAC(2/6)
42 GTCTTC
BpuAI 619 GAAGAC GAAGAC(2/6)
42 GTCTTC
BssKI 48 CCAGG ^CC[ACGT]GG
123 CCAGG
BstNI 48 CCAGG CC^[AT]GG
123 CCAGG
EaeI 113 CGGCCA [CT]^GGCC[GA]
149 TGGCCA
EcoRII 48 CCAGG ^CC[AT]GG
123 CCAGG
HaeIII 114 GGCC GG^CC
150 GGCC
HapII 1344 CCGG C^CGG
1466 CCGG
HpaII 1344 CCGG C^CGG
1466 CCGG
HphI 745 GGTGA GGTGA(8/7)
755 TCACC
MspI 1344 CCGG C^CGG
1466 CCGG
MvaI 48 CCAGG CC^[AT]GG
123 CCAGG
MwoI 899 GCAGTGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1505 GCTTTAAGTGC
NlaIV 186 GGATCC GG[ACGT]^[ACGT]CC
224 GGAACC
PleI 445 GACTC GAGTC(4/5)
788 GAGTC
RsaI 1013 GTAC GT^AC
1395 GTAC
SacI 35 GAGCTC GAGCT^C
1347 GAGCTC
SapI 72 GAAGAGC GCTCTTC(1/4)
1552 GAAGAGC
ScrFI 48 CCAGG CC^[ACGT]GG
123 CCAGG
TaqI 777 TCGA T^CGA
1008 TCGA
TthHB8I 777 TCGA T^CGA
1008 TCGA
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
AspHI BbvI BfaI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I BsrDI BsrI
BstF5I BstYI DdeI DpnII EarI
EcoRI Fnu4HI FokI HindIII HinfI
ItaI Ksp632I MaeI MboI MboII
MflI MnlI MseI MslI NdeII
NlaIII Sau3AI SfaNI TfiI Tru9I
TseI Tsp509I TspRI XhoII XmnI