Restriction map for
pF1KSDA1860
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Aor51HI ApoI
AscI AseI AsnI Asp718I AspI
AviII AvrII BcgI BciVI BclI
BfrI BglII BlnI BsaI BsaWI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspMI BsrDI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MfeI MluI MroI MseI
MslI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I RsrII SacI SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tru9I Tth111I Van91I XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AhdI 1987 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 505 GTGCAC G^TGCAC
AlwNI 301 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1989 CCTGAGG CC^T[ACGT]AGG
ApaI 1768 GGGCCC GGGCC^C
ApaLI 505 GTGCAC G^TGCAC
AspEI 1987 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1713 TGGCCA TGG^CCA
BamHI 1945 GGATCC G^GATCC
BbrPI 163 CACGTG CAC^GTG
BbvCI 183 GCTGAGG CCTCAGC(-5/-2)
BfaI 1508 CTAG C^TAG
BlpI 1558 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1558 GCTGAGC GC^T[ACGT]AGC
BsaAI 163 CACGTG [CT]AC^GT[GA]
BsaBI 927 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 33 CGATCG CG[GA][CT]^CG
BsmAI 376 GAGAC GTCTC(1/5)
BspLU11I 833 ACATGT A^CATGT
BsrFI 1727 ACCGGC [GA]^CCGG[CT]
BstEII 1878 GGTTACC G^GT[ACGT]ACC
BstPI 1878 GGTTACC G^GT[ACGT]ACC
BstXI 1415 CCAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1989 CCTGAGG CC^T[ACGT]AGG
CelII 1558 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1727 ACCGGC [GA]^CCGG[CT]
DraIII 110 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1987 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1989 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1878 GGTTACC G^GT[ACGT]ACC
HgaI 387 GACGC GACGC(5/10)
HincII 837 GTCAAC GT[CT]^[GA]AC
HindII 837 GTCAAC GT[CT]^[GA]AC
KspI 1382 CCGCGG CCGC^GG
MaeI 1508 CTAG C^TAG
MamI 927 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1713 TGGCCA TGG^CCA
MscI 1713 TGGCCA TGG^CCA
NdeI 659 CATATG CA^TATG
NspI 833 ACATGT [GA]CATG^[CT]
PmaCI 163 CACGTG CAC^GTG
PmlI 163 CACGTG CAC^GTG
PvuI 33 CGATCG CGAT^CG
PvuII 280 CAGCTG CAG^CTG
SacII 1382 CCGCGG CCGC^GG
ScaI 801 AGTACT AGT^ACT
SexAI 395 ACCTGGT A^CC[AT]GGT
SspI 526 AATATT AAT^ATT
Tsp509I 1002 AATT ^AATT
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 638 ACACGT A^C[GA][CT]GT
833 ACATGT
Asp700I 303 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1187 GAACAAGTTC
BglI 1792 GCCCATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1812 GCCCGCCGGGC
Bsp1407I 531 TGTACA T^GTACA
737 TGTACA
BspHI 326 TCATGA T^CATGA
911 TCATGA
BsrGI 531 TGTACA T^GTACA
737 TGTACA
BssSI 1482 CACGAG CACGAG(-5/-1)
439 CTCGTG
BstYI 1457 AGATCC [GA]^GATC[CT]
1945 GGATCC
EarI 1236 CTCTTC CTCTTC(1/4)
1843 CTCTTC
Ksp632I 1236 CTCTTC CTCTTC(1/4)
1843 CTCTTC
MflI 1457 AGATCC [GA]^GATC[CT]
1945 GGATCC
NcoI 1421 CCATGG C^CATGG
1707 CCATGG
PaeR7I 473 CTCGAG C^TCGAG
901 CTCGAG
PstI 298 CTGCAG CTGCA^G
1374 CTGCAG
RcaI 326 TCATGA T^CATGA
911 TCATGA
SfcI 298 CTGCAG C^T[GA][CT]AG
1374 CTGCAG
SmaI 24 CCCGGG CCC^GGG
1551 CCCGGG
SspBI 531 TGTACA T^GTACA
737 TGTACA
XhoI 473 CTCGAG C^TCGAG
901 CTCGAG
XhoII 1457 AGATCC [GA]^GATC[CT]
1945 GGATCC
XmaCI 24 CCCGGG C^CCGGG
1551 CCCGGG
XmaI 24 CCCGGG C^CCGGG
1551 CCCGGG
XmnI 303 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1187 GAACAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbeI BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfoI SmlI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XcmI