Restriction map for
pF1KSDA1817
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbeI BbsI BciVI BclI
BfrI BlnI BpuAI BsaBI BsaHI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FbaI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NarI NdeI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
RcaI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspBI SspI
SwaI XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1501 GTCTAC GT^[AC][GT]AC
AflIII 1390 ACGTGT A^C[GA][CT]GT
AlwI 1836 GATCC GGATC(4/5)
AocI 1930 CCTGAGG CC^T[ACGT]AGG
ApaI 2009 GGGCCC GGGCC^C
AspI 1243 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1389 CACGTG CAC^GTG
BcgI 2103 CGAGGCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2135 AGATCT A^GATCT
BlpI 2262 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2262 GCTGAGC GC^T[ACGT]AGC
BsaAI 1389 CACGTG [CT]AC^GT[GA]
BsaWI 1694 ACCGGA [AT]^CCGG[AT]
BsmI 1319 GCATTC GAATGC(1/-1)
BsrBI 967 GAGCGG CCGCTC(-3/-3)
Bsu36I 1930 CCTGAGG CC^T[ACGT]AGG
CelII 2262 GCTGAGC GC^T[ACGT]AGC
DsaI 656 CCATGG C^C[GA][CT]GG
EagI 137 CGGCCG C^GGCCG
EclXI 137 CGGCCG C^GGCCG
Eco52I 137 CGGCCG C^GGCCG
Eco81I 1930 CCTGAGG CC^T[ACGT]AGG
EcoNI 63 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 327 GAATTC G^AATTC
FseI 1189 GGCCGGCC GGCCGG^CC
HaeII 2321 AGCGCC [GA]GCGC^[CT]
HgaI 1605 GACGC GACGC(5/10)
MslI 2014 CATGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 656 CCATGG C^CATGG
NheI 2157 GCTAGC G^CTAGC
PflFI 1243 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1389 CACGTG CAC^GTG
PmlI 1389 CACGTG CAC^GTG
PvuI 1354 CGATCG CGAT^CG
SapI 34 GAAGAGC GCTCTTC(1/4)
SbfI 1890 CCTGCAGG CCTGCA^GG
SphI 1523 GCATGC GCATG^C
Sse8387I 1890 CCTGCAGG CCTGCA^GG
StuI 1934 AGGCCT AGG^CCT
TfiI 282 GAATC G^A[AT]TC
Tth111I 1243 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 313 CCAGAGACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 327 GAATTC [GA]^AATT[CT]
438 AAATTT
AfaI 1065 GTAC GT^AC
1692 GTAC
ApoI 327 GAATTC [GA]^AATT[CT]
438 AAATTT
BglI 1181 GCCGGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2002 GCCCTGCGGGC
BseRI 1684 GAGGAG GAGGAG(10/8)
2389 GAGGAG
BsiEI 137 CGGCCG CG[GA][CT]^CG
1354 CGATCG
BsrFI 803 GCCGGC [GA]^CCGG[CT]
1190 GCCGGC
BstYI 1835 AGATCC [GA]^GATC[CT]
2135 AGATCT
Cfr10I 803 GCCGGC [GA]^CCGG[CT]
1190 GCCGGC
MaeII 1390 ACGT A^CGT
1625 ACGT
MflI 1835 AGATCC [GA]^GATC[CT]
2135 AGATCT
NaeI 803 GCCGGC GCC^GGC
1190 GCCGGC
NgoMIV 803 GCCGGC G^CCGGC
1190 GCCGGC
PaeR7I 740 CTCGAG C^TCGAG
767 CTCGAG
RsaI 1065 GTAC GT^AC
1692 GTAC
XhoI 740 CTCGAG C^TCGAG
767 CTCGAG
XhoII 1835 AGATCC [GA]^GATC[CT]
2135 AGATCT
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I