Restriction map for
pF1KSDA1798
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII AccIII AclI AcyI AgeI
Alw44I AlwNI Aor51HI ApaLI AscI
AspI AvaII AviII AvrII BbeI
BbrPI BbvCI BcgI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT14I EcoT22I FauI FseI FspI
HaeII Hin1I HincII HindII HpaI
KasI KspI MluI MroI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 195 GGTACC G^GTACC
AccII 858 CGCG CG^CG
AflIII 1042 ACATGT A^C[GA][CT]GT
AhdI 2131 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 606 CCTGAGG CC^T[ACGT]AGG
AseI 1644 ATTAAT AT^TAAT
AsnI 1644 ATTAAT AT^TAAT
Asp718I 195 GGTACC G^GTACC
AspEI 2131 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1698 TGGCCA TGG^CCA
BciVI 871 GGATAC GTATCC(6/5)
BclI 1503 TGATCA T^GATCA
BsaBI 445 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1886 GAGACC GGTCTC(1/5)
BsaWI 875 ACCGGA [AT]^CCGG[AT]
BsmI 927 GAATGC GAATGC(1/-1)
BspLU11I 1042 ACATGT A^CATGT
BstAPI 663 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 858 CGCG CG^CG
Bsu36I 606 CCTGAGG CC^T[ACGT]AGG
CfoI 2055 GCGC GCG^C
DraI 2188 TTTAAA TTT^AAA
Eam1105I 2131 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 606 CCTGAGG CC^T[ACGT]AGG
EcoRV 943 GATATC GAT^ATC
FbaI 1503 TGATCA T^GATCA
HhaI 2055 GCGC GCG^C
HinP1I 2055 GCGC G^CGC
KpnI 195 GGTACC GGTAC^C
MamI 445 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1698 TGGCCA TGG^CCA
MscI 1698 TGGCCA TGG^CCA
MspA1I 2009 CAGCTG C[AC]G^C[GT]G
MvnI 858 CGCG CG^CG
NaeI 738 GCCGGC GCC^GGC
NgoMIV 738 GCCGGC G^CCGGC
PflMI 182 CCACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 2229 GACTC GAGTC(4/5)
PshBI 1644 ATTAAT AT^TAAT
PstI 2312 CTGCAG CTGCA^G
PvuII 2009 CAGCTG CAG^CTG
SacI 231 GAGCTC GAGCT^C
SapI 1865 GCTCTTC GCTCTTC(1/4)
SexAI 1317 ACCAGGT A^CC[AT]GGT
SphI 1893 GCATGC GCATG^C
TaqI 1113 TCGA T^CGA
Tsp45I 92 GTGAC ^GT[GC]AC
TthHB8I 1113 TCGA T^CGA
Van91I 182 CCACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 1138 GTAGAC GT^[AC][GT]AC
1450 GTAGAC
AflII 1036 CTTAAG C^TTAAG
1797 CTTAAG
ApaI 1725 GGGCCC GGGCC^C
2268 GGGCCC
BamHI 327 GGATCC G^GATCC
819 GGATCC
BanI 195 GGTACC G^G[CT][GA]CC
1966 GGTGCC
BfaI 17 CTAG C^TAG
1820 CTAG
BfrI 1036 CTTAAG C^TTAAG
1797 CTTAAG
BpmI 234 CTCCAG CTGGAG(16/14)
401 CTGGAG
BseRI 1412 CTCCTC GAGGAG(10/8)
718 GAGGAG
BsmAI 1716 GAGAC GTCTC(1/5)
1886 GAGAC
BspMI 252 ACCTGC ACCTGC(4/8)
1380 ACCTGC
BsrFI 738 GCCGGC [GA]^CCGG[CT]
973 ACCGGC
Cfr10I 738 GCCGGC [GA]^CCGG[CT]
973 ACCGGC
DraII 1725 GGGCCCT [GA]G^G[ACGT]CC[CT]
2268 GGGCCCT
EaeI 975 CGGCCA [CT]^GGCC[GA]
1698 TGGCCA
EcoO109I 1725 GGGCCCT [GA]G^G[ACGT]CC[CT]
2268 GGGCCCT
EcoRI 1015 GAATTC G^AATTC
2322 GAATTC
HgaI 934 GACGC GACGC(5/10)
2001 GCGTC
MaeI 17 CTAG C^TAG
1820 CTAG
MfeI 1020 CAATTG C^AATTG
1539 CAATTG
MunI 1020 CAATTG C^AATTG
1539 CAATTG
NspI 1042 ACATGT [GA]CATG^[CT]
1893 GCATGC
SfcI 1586 CTGTAG C^T[GA][CT]AG
2312 CTGCAG
SmaI 1680 CCCGGG CCC^GGG
1970 CCCGGG
XcmI 182 CCACCACTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
250 CCACCTGCCTACTGG
XmaCI 1680 CCCGGG C^CCGGG
1970 CCCGGG
XmaI 1680 CCCGGG C^CCGGG
1970 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaI BanII
BbsI BbvI BcnI BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoNI EcoRII
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI TfiI Tru9I TseI Tsp509I
TspRI XhoII XmnI