Restriction map for
pF1KSDA1792
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
BalI BamHI BbeI BbsI BcnI
BpuAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CfoI
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FseI FspI HaeII
HhaI Hin1I HinP1I KasI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NarI NciI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 955 GGTACC G^GTACC
AflII 1531 CTTAAG C^TTAAG
ApaI 1714 GGGCCC GGGCC^C
Asp718I 955 GGTACC G^GTACC
AspHI 510 GAGCTC G[AT]GC[AT]^C
AvrII 775 CCTAGG C^CTAGG
BanI 955 GGTACC G^G[CT][GA]CC
BbrPI 1099 CACGTG CAC^GTG
BbvCI 1144 CCTCAGC CCTCAGC(-5/-2)
BciVI 880 GTATCC GTATCC(6/5)
BfrI 1531 CTTAAG C^TTAAG
BglII 612 AGATCT A^GATCT
BlnI 775 CCTAGG C^CTAGG
BlpI 507 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 507 GCTGAGC GC^T[ACGT]AGC
BsaAI 1099 CACGTG [CT]AC^GT[GA]
BsiHKAI 510 GAGCTC G[AT]GC[AT]^C
BsmI 1233 GAATGC GAATGC(1/-1)
BsrFI 1824 GCCGGC [GA]^CCGG[CT]
BstAPI 1237 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 532 CATCC GGATG(2/0)
CelII 507 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1824 GCCGGC [GA]^CCGG[CT]
DrdI 1765 GACTTAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1527 CCTCCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 392 ATGCAT ATGCA^T
FauI 2056 CCCGC CCCGC(4/6)
FokI 532 CATCC GGATG(9/13)
HapII 1825 CCGG C^CGG
HgaI 1246 GCGTC GACGC(5/10)
HincII 1786 GTTAAC GT[CT]^[GA]AC
HindII 1786 GTTAAC GT[CT]^[GA]AC
HindIII 632 AAGCTT A^AGCTT
HpaI 1786 GTTAAC GTT^AAC
HpaII 1825 CCGG C^CGG
KpnI 955 GGTACC GGTAC^C
MaeIII 1896 GTTAC ^GT[ACGT]AC
MspI 1825 CCGG C^CGG
NaeI 1824 GCCGGC GCC^GGC
NgoMIV 1824 GCCGGC G^CCGGC
NsiI 392 ATGCAT ATGCA^T
PmaCI 1099 CACGTG CAC^GTG
PmlI 1099 CACGTG CAC^GTG
PstI 521 CTGCAG CTGCA^G
SacI 510 GAGCTC GAGCT^C
SphI 2066 GCATGC GCATG^C
SspI 1072 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 24 enzymes) #
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Asp700I 1151 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2271 GAAAGTATTC
BcgI 40 CGAGCATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2169 GCAGCTGGCTCG
BclI 617 TGATCA T^GATCA
935 TGATCA
BglI 1424 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1819 GCCACGCCGGC
BsaI 685 GAGACC GGTCTC(1/5)
1699 GAGACC
BseRI 1142 CTCCTC GAGGAG(10/8)
946 GAGGAG
BsgI 2149 CTGCAC GTGCAG(16/14)
68 GTGCAG
BsmFI 117 GGGAC GGGAC(10/14)
1312 GGGAC
BspHI 1118 TCATGA T^CATGA
2265 TCATGA
BspMI 1412 ACCTGC ACCTGC(4/8)
1420 ACCTGC
BsrDI 269 CATTGC GCAATG(2/0)
2009 GCAATG
DraI 783 TTTAAA TTT^AAA
976 TTTAAA
DsaI 1961 CCATGG C^C[GA][CT]GG
2033 CCATGG
FbaI 617 TGATCA T^GATCA
935 TGATCA
MslI 1095 CACTCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2034 CATGGAGATG
NcoI 1961 CCATGG C^CATGG
2033 CCATGG
NdeI 174 CATATG CA^TATG
1735 CATATG
PpuMI 122 AGGACCC [GA]G^G[AT]CC[CT]
887 AGGACCT
RcaI 1118 TCATGA T^CATGA
2265 TCATGA
SfcI 355 CTACAG C^T[GA][CT]AG
521 CTGCAG
TaqI 1275 TCGA T^CGA
1550 TCGA
TthHB8I 1275 TCGA T^CGA
1550 TCGA
XcmI 1418 CCACCTGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2115 CCAGTATTGCTATGG
XmnI 1151 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2271 GAAAGTATTC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanII BbvI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SmlI StyI TfiI Tru9I
TseI Tsp509I TspRI XhoII