Restriction map for
pF1KSDA1787
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# NO CUT ENZYMES ( 58 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AscI Asp718I AspI AvrII
BamHI BbeI BclI BfrI BlnI
BsmI Bsp1407I BspLU11I BsrGI Bst1107I
BstBI BstZ17I CpoI DraI EcoRV
EcoT22I FbaI FseI HpaI KasI
KpnI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
RsrII SalI SbfI SfoI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 3002 TCCGGA T^CCGGA
AhdI 2311 GACCGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 321 AGCGCT AGC^GCT
AseI 1348 ATTAAT AT^TAAT
AsnI 1348 ATTAAT AT^TAAT
Asp700I 2700 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2311 GACCGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3714 CACGTG CAC^GTG
BciVI 3025 GTATCC GTATCC(6/5)
BglII 3683 AGATCT A^GATCT
BsaAI 3714 CACGTG [CT]AC^GT[GA]
BsaWI 3002 TCCGGA [AT]^CCGG[AT]
BseAI 3002 TCCGGA T^CCGGA
BspDI 1357 ATCGAT AT^CGAT
BspEI 3002 TCCGGA T^CCGGA
BssHII 3587 GCGCGC G^CGCGC
ClaI 1357 ATCGAT AT^CGAT
DraIII 1651 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 754 CGGCCG C^GGCCG
Eam1105I 2311 GACCGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 754 CGGCCG C^GGCCG
Eco47III 321 AGCGCT AGC^GCT
Eco52I 754 CGGCCG C^GGCCG
HincII 283 GTCAAC GT[CT]^[GA]AC
HindII 283 GTCAAC GT[CT]^[GA]AC
HindIII 1105 AAGCTT A^AGCTT
KspI 3234 CCGCGG CCGC^GG
MfeI 3245 CAATTG C^AATTG
MluI 252 ACGCGT A^CGCGT
MroI 3002 TCCGGA T^CCGGA
MunI 3245 CAATTG C^AATTG
NaeI 1016 GCCGGC GCC^GGC
NgoMIV 1016 GCCGGC G^CCGGC
PmaCI 3714 CACGTG CAC^GTG
PmlI 3714 CACGTG CAC^GTG
PshBI 1348 ATTAAT AT^TAAT
SacII 3234 CCGCGG CCGC^GG
SexAI 2102 ACCAGGT A^CC[AT]GGT
SfiI 3352 GGCCTGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1015 CGCCGGCG C[GA]^CCGG[CT]G
XbaI 2775 TCTAGA T^CTAGA
XmnI 2700 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AviII 1703 TGCGCA TGC^GCA
2450 TGCGCA
BbvCI 2847 GCTGAGG CCTCAGC(-5/-2)
2854 GCTGAGG
BlpI 1009 GCTGAGC GC^T[ACGT]AGC
1662 GCTGAGC
Bpu1102I 1009 GCTGAGC GC^T[ACGT]AGC
1662 GCTGAGC
BsaBI 1092 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3141 GATGCACATC
BsaI 4462 GAGACC GGTCTC(1/5)
3676 GGTCTC
BsiEI 273 CGACCG CG[GA][CT]^CG
754 CGGCCG
BsiWI 175 CGTACG C^GTACG
3445 CGTACG
BsrDI 3189 CATTGC GCAATG(2/0)
4268 GCAATG
BstAPI 2797 GCAGTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4315 GCAGGTACTGC
CelII 1009 GCTGAGC GC^T[ACGT]AGC
1662 GCTGAGC
DrdI 274 GACCGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2239 GACGCCATCGTC
EarI 1564 CTCTTC CTCTTC(1/4)
2881 GAAGAG
FspI 1703 TGCGCA TGC^GCA
2450 TGCGCA
Ksp632I 1564 CTCTTC CTCTTC(1/4)
2881 GAAGAG
MamI 1092 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3141 GATGCACATC
NcoI 2660 CCATGG C^CATGG
3411 CCATGG
PaeR7I 664 CTCGAG C^TCGAG
3394 CTCGAG
PshAI 92 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1437 GACCATCGTC
PstI 716 CTGCAG CTGCA^G
3176 CTGCAG
SacI 876 GAGCTC GAGCT^C
2998 GAGCTC
SapI 2881 GAAGAGC GCTCTTC(1/4)
1563 GCTCTTC
ScaI 1262 AGTACT AGT^ACT
2752 AGTACT
SfcI 716 CTGCAG C^T[GA][CT]AG
3176 CTGCAG
StuI 549 AGGCCT AGG^CCT
897 AGGCCT
XhoI 664 CTCGAG C^TCGAG
3394 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrFI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI