Restriction map for
pF1KSDA1759
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbeI BbrPI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII BstBI ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MluI
MroI MseI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I Tth111I Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccII 753 CGCG CG^CG
AcsI 2797 GAATTT [GA]^AATT[CT]
ApoI 2797 GAATTT [GA]^AATT[CT]
AviII 206 TGCGCA TGC^GCA
BalI 782 TGGCCA TGG^CCA
BsmBI 2657 GAGACG CGTCTC(1/5)
BsmI 2766 GAATGC GAATGC(1/-1)
BspHI 899 TCATGA T^CATGA
Bst1107I 53 GTATAC GTA^TAC
BstEII 1882 GGTCACC G^GT[ACGT]ACC
BstPI 1882 GGTCACC G^GT[ACGT]ACC
BstUI 753 CGCG CG^CG
BstZ17I 53 GTATAC GTA^TAC
CfoI 207 GCGC GCG^C
DraIII 1673 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1687 GACCGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2490 CCGTGG C^C[GA][CT]GG
EcoO65I 1882 GGTCACC G^GT[ACGT]ACC
FspI 206 TGCGCA TGC^GCA
HhaI 207 GCGC GCG^C
HinP1I 207 GCGC G^CGC
MfeI 2635 CAATTG C^AATTG
MluNI 782 TGGCCA TGG^CCA
MscI 782 TGGCCA TGG^CCA
MunI 2635 CAATTG C^AATTG
MvnI 753 CGCG CG^CG
NaeI 1311 GCCGGC GCC^GGC
NgoMIV 1311 GCCGGC G^CCGGC
RcaI 899 TCATGA T^CATGA
SacI 2029 GAGCTC GAGCT^C
SexAI 1711 ACCTGGT A^CC[AT]GGT
SfiI 2301 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 2117 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 53 GTATAC GT^[AC][GT]AC
2773 GTCTAC
AocI 1795 CCTGAGG CC^T[ACGT]AGG
1927 CCTGAGG
AspHI 2029 GAGCTC G[AT]GC[AT]^C
2230 GTGCTC
BsaI 100 GAGACC GGTCTC(1/5)
1200 GGTCTC
BsiHKAI 2029 GAGCTC G[AT]GC[AT]^C
2230 GTGCTC
BsrFI 1130 ACCGGC [GA]^CCGG[CT]
1311 GCCGGC
BssSI 304 CACGAG CACGAG(-5/-1)
1430 CACGAG
BstAPI 849 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2524 GCAGGACCTGC
BstXI 1899 CCAGCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1915 CCAGGATCATGG
BstYI 309 GGATCT [GA]^GATC[CT]
2875 GGATCT
Bsu36I 1795 CCTGAGG CC^T[ACGT]AGG
1927 CCTGAGG
Cfr10I 1130 ACCGGC [GA]^CCGG[CT]
1311 GCCGGC
Eco81I 1795 CCTGAGG CC^T[ACGT]AGG
1927 CCTGAGG
EcoT14I 2576 CCTTGG C^C[AT][AT]GG
2742 CCAAGG
MflI 309 GGATCT [GA]^GATC[CT]
2875 GGATCT
PaeR7I 580 CTCGAG C^TCGAG
2245 CTCGAG
SmaI 35 CCCGGG CCC^GGG
2720 CCCGGG
SphI 1416 GCATGC GCATG^C
2598 GCATGC
StyI 2576 CCTTGG C^C[AT][AT]GG
2742 CCAAGG
XcmI 835 CCAGGGGATGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2202 CCAGCCCTTCACTGG
XhoI 580 CTCGAG C^TCGAG
2245 CTCGAG
XhoII 309 GGATCT [GA]^GATC[CT]
2875 GGATCT
XmaCI 35 CCCGGG C^CCGGG
2720 CCCGGG
XmaI 35 CCCGGG C^CCGGG
2720 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstF5I BstNI Cac8I CelII
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I