Restriction map for
pF1KSDA1754
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstYI BstZ17I ClaI CpoI DraI
DraIII DrdI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MaeI MaeII MamI MfeI MflI
MroI MseI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I XbaI
XcmI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AgeI 1267 ACCGGT A^CCGGT
AlwNI 927 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 328 GGGCCC GGGCC^C
Asp700I 903 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 1215 CCTCAGC CCTCAGC(-5/-2)
BlpI 1281 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1281 GCTCAGC GC^T[ACGT]AGC
BsaWI 1267 ACCGGT [AT]^CCGG[AT]
BsmI 1575 GAATGC GAATGC(1/-1)
BspHI 899 TCATGA T^CATGA
BspLU11I 1608 ACATGT A^CATGT
BsrBI 690 CCGCTC CCGCTC(-3/-3)
BsrFI 1267 ACCGGT [GA]^CCGG[CT]
BssSI 1375 CACGAG CACGAG(-5/-1)
BstXI 670 CCAGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1281 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1267 ACCGGT [GA]^CCGG[CT]
HgaI 1363 GACGC GACGC(5/10)
KspI 70 CCGCGG CCGC^GG
MaeIII 37 GTGAC ^GT[ACGT]AC
MluI 246 ACGCGT A^CGCGT
PflMI 926 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1267 ACCGGT A^CCGGT
PshAI 1363 GACGCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 899 TCATGA T^CATGA
SacI 673 GAGCTC GAGCT^C
SacII 70 CCGCGG CCGC^GG
SmlI 1570 CTCAAG C^T[CT][GA]AG
Tsp45I 37 GTGAC ^GT[GC]AC
Van91I 926 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 903 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 246 ACGCGT A^C[GA][CT]GT
1608 ACATGT
Aor51HI 440 AGCGCT AGC^GCT
1584 AGCGCT
BbsI 790 GAAGAC GAAGAC(2/6)
1299 GAAGAC
BpuAI 790 GAAGAC GAAGAC(2/6)
1299 GAAGAC
BstAPI 269 GCACCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1374 GCACGAGTTGC
Eco47III 440 AGCGCT AGC^GCT
1584 AGCGCT
EcoNI 162 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1326 CCTCCGGCAGG
EcoT14I 5 CCATGG C^C[AT][AT]GG
1460 CCATGG
NcoI 5 CCATGG C^CATGG
1460 CCATGG
PpuMI 257 GGGACCT [GA]G^G[AT]CC[CT]
994 GGGTCCC
SapI 1398 GAAGAGC GCTCTTC(1/4)
12 GCTCTTC
SmaI 479 CCCGGG CCC^GGG
990 CCCGGG
StyI 5 CCATGG C^C[AT][AT]GG
1460 CCATGG
TaqI 299 TCGA T^CGA
962 TCGA
TfiI 871 GATTC G^A[AT]TC
1044 GATTC
TthHB8I 299 TCGA T^CGA
962 TCGA
XmaCI 479 CCCGGG C^CCGGG
990 CCCGGG
XmaI 479 CCCGGG C^CCGGG
990 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI AspHI AvaI AvaII
BalI BanI BanII BbeI BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI TseI
TspRI