Restriction map for
pF1KSDA1750
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI MaeIII MamI MfeI MflI
MluI MroI MslI MunI NarI
NcoI NdeI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tsp45I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcyI 91 GACGCC G[GA]^CG[CT]C
AlwNI 353 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 478 CCTCAGG CC^T[ACGT]AGG
AspI 355 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 954 TGGCCA TGG^CCA
BcgI 328 GCATCTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 606 GGATAC GTATCC(6/5)
BfaI 784 CTAG C^TAG
BsaHI 91 GACGCC G[GA]^CG[CT]C
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BssSI 1160 CTCGTG CACGAG(-5/-1)
BstXI 979 CCAATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 478 CCTCAGG CC^T[ACGT]AGG
EagI 6 CGGCCG C^GGCCG
EclXI 6 CGGCCG C^GGCCG
Eco52I 6 CGGCCG C^GGCCG
Eco81I 478 CCTCAGG CC^T[ACGT]AGG
EcoNI 1014 CCTAAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 341 AGCGCC [GA]GCGC^[CT]
Hin1I 91 GACGCC G[GA]^CG[CT]C
HphI 1236 GGTGA GGTGA(8/7)
MaeI 784 CTAG C^TAG
MaeII 620 ACGT A^CGT
MluNI 954 TGGCCA TGG^CCA
MscI 954 TGGCCA TGG^CCA
MseI 829 TTAA T^TAA
NaeI 236 GCCGGC GCC^GGC
NgoMIV 236 GCCGGC G^CCGGC
NheI 783 GCTAGC G^CTAGC
PflFI 355 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 703 CTGCAG CTGCA^G
PvuI 894 CGATCG CGAT^CG
SacI 850 GAGCTC GAGCT^C
SapI 847 GAAGAGC GCTCTTC(1/4)
Tru9I 829 TTAA T^TAA
Tsp509I 1115 AATT ^AATT
Tth111I 355 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 120 GTAC GT^AC
817 GTAC
ApaI 269 GGGCCC GGGCC^C
580 GGGCCC
AspHI 850 GAGCTC G[AT]GC[AT]^C
922 GTGCTC
BbsI 136 GAAGAC GAAGAC(2/6)
361 GTCTTC
BpuAI 136 GAAGAC GAAGAC(2/6)
361 GTCTTC
BseRI 208 CTCCTC GAGGAG(10/8)
223 GAGGAG
BsgI 157 GTGCAG GTGCAG(16/14)
622 GTGCAG
BsiEI 6 CGGCCG CG[GA][CT]^CG
894 CGATCG
BsiHKAI 850 GAGCTC G[AT]GC[AT]^C
922 GTGCTC
BsmFI 284 GGGAC GGGAC(10/14)
1011 GTCCC
BsrFI 236 GCCGGC [GA]^CCGG[CT]
419 ACCGGC
BsrI 984 CCAGT ACTGG(1/-1)
1008 CCAGT
Cfr10I 236 GCCGGC [GA]^CCGG[CT]
419 ACCGGC
DpnII 895 GATC ^GATC
1003 GATC
EarI 559 GAAGAG CTCTTC(1/4)
847 GAAGAG
HgaI 91 GACGC GACGC(5/10)
196 GCGTC
Ksp632I 559 GAAGAG CTCTTC(1/4)
847 GAAGAG
KspI 299 CCGCGG CCGC^GG
464 CCGCGG
MboI 895 GATC ^GATC
1003 GATC
NdeII 895 GATC ^GATC
1003 GATC
PvuII 539 CAGCTG CAG^CTG
625 CAGCTG
RsaI 120 GTAC GT^AC
817 GTAC
SacII 299 CCGCGG CCGC^GG
464 CCGCGG
Sau3AI 895 GATC ^GATC
1003 GATC
SfcI 456 CTGTAG C^T[GA][CT]AG
703 CTGCAG
SmaI 315 CCCGGG CCC^GGG
744 CCCGGG
TaqI 21 TCGA T^CGA
893 TCGA
TspRI 357 CACTG CA[GC]TG[ACGT][ACGT]^
985 CAGTG
TthHB8I 21 TCGA T^CGA
893 TCGA
XmaCI 315 CCCGGG C^CCGGG
744 CCCGGG
XmaI 315 CCCGGG C^CCGGG
744 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsoBI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
Sau96I ScrFI SfaNI TseI