Restriction map for
pF1KSDA1748
##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspI
AvrII BamHI BbeI BciVI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsiWI BsmBI BspEI BspHI BspLU11I
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
Cfr10I CpoI DraI DrdI DsaI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI HindIII HpaI KasI
KspI MamI MflI MluI MroI
MseI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspI StuI
SwaI Tru9I Tth111I XbaI XhoII
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 2122 GGTACC G^GTACC
AccII 1610 CGCG CG^CG
AcyI 1802 GACGCC G[GA]^CG[CT]C
AflIII 103 ACGTGT A^C[GA][CT]GT
AhdI 1600 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1255 GATCC GGATC(4/5)
Asp700I 687 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2122 GGTACC G^GTACC
AspEI 1600 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 260 GAGCTC G[AT]GC[AT]^C
AviII 302 TGCGCA TGC^GCA
BbrPI 619 CACGTG CAC^GTG
BcgI 1329 GCAATCCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 390 TGATCA T^GATCA
BlpI 2042 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2042 GCTCAGC GC^T[ACGT]AGC
BsaHI 1802 GACGCC G[GA]^CG[CT]C
BsgI 1453 GTGCAG GTGCAG(16/14)
BsiHKAI 260 GAGCTC G[AT]GC[AT]^C
Bsp1407I 1052 TGTACA T^GTACA
BspDI 1899 ATCGAT AT^CGAT
BsrBI 1188 CCGCTC CCGCTC(-3/-3)
BsrDI 1391 GCAATG GCAATG(2/0)
BsrGI 1052 TGTACA T^GTACA
BssSI 2085 CACGAG CACGAG(-5/-1)
BstUI 1610 CGCG CG^CG
CelII 2042 GCTCAGC GC^T[ACGT]AGC
ClaI 1899 ATCGAT AT^CGAT
DraIII 1615 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1063 CGGCCG C^GGCCG
Eam1105I 1600 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 88 CTCTTC CTCTTC(1/4)
EclXI 1063 CGGCCG C^GGCCG
Eco52I 1063 CGGCCG C^GGCCG
EcoT14I 1025 CCAAGG C^C[AT][AT]GG
FauI 4 CCCGC CCCGC(4/6)
FbaI 390 TGATCA T^GATCA
FspI 302 TGCGCA TGC^GCA
HaeII 1114 AGCGCC [GA]GCGC^[CT]
Hin1I 1802 GACGCC G[GA]^CG[CT]C
HincII 1716 GTCAAC GT[CT]^[GA]AC
HindII 1716 GTCAAC GT[CT]^[GA]AC
KpnI 2122 GGTACC GGTAC^C
Ksp632I 88 CTCTTC CTCTTC(1/4)
MvnI 1610 CGCG CG^CG
PaeR7I 221 CTCGAG C^TCGAG
PmaCI 619 CACGTG CAC^GTG
PmlI 619 CACGTG CAC^GTG
PpuMI 1858 GGGTCCC [GA]G^G[AT]CC[CT]
PvuI 1897 CGATCG CGAT^CG
SacI 260 GAGCTC GAGCT^C
SexAI 1929 ACCTGGT A^CC[AT]GGT
SmaI 1671 CCCGGG CCC^GGG
SpeI 1747 ACTAGT A^CTAGT
SspBI 1052 TGTACA T^GTACA
StyI 1025 CCAAGG C^C[AT][AT]GG
XhoI 221 CTCGAG C^TCGAG
XmaCI 1671 CCCGGG C^CCGGG
XmaI 1671 CCCGGG C^CCGGG
XmnI 687 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 385 GAATTT [GA]^AATT[CT]
670 AAATTC
ApoI 385 GAATTT [GA]^AATT[CT]
670 AAATTC
BbsI 777 GAAGAC GAAGAC(2/6)
1998 GAAGAC
BbvCI 226 GCTGAGG CCTCAGC(-5/-2)
870 GCTGAGG
BfaI 70 CTAG C^TAG
1748 CTAG
BglI 1510 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1830 GCCAGATGGGC
BpuAI 777 GAAGAC GAAGAC(2/6)
1998 GAAGAC
BsaAI 102 TACGTG [CT]AC^GT[GA]
619 CACGTG
BsaI 762 GAGACC GGTCTC(1/5)
48 GGTCTC
BsiEI 1063 CGGCCG CG[GA][CT]^CG
1897 CGATCG
BsmI 1104 GCATTC GAATGC(1/-1)
1709 GCATTC
BspMI 132 ACCTGC ACCTGC(4/8)
322 ACCTGC
BstXI 194 CCACCTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
749 CCAGGTATTTGG
DraII 1461 AGGGCCT [GA]G^G[ACGT]CC[CT]
1858 GGGTCCC
EcoNI 1864 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2053 CCTGGCCCAGG
EcoO109I 1461 AGGGCCT [GA]G^G[ACGT]CC[CT]
1858 GGGTCCC
HgaI 641 GACGC GACGC(5/10)
1802 GACGC
MaeI 70 CTAG C^TAG
1748 CTAG
MfeI 782 CAATTG C^AATTG
1157 CAATTG
MunI 782 CAATTG C^AATTG
1157 CAATTG
PflMI 474 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1614 CCACATTGTGG
SmlI 221 CTCGAG C^T[CT][GA]AG
1288 CTCAAG
TfiI 23 GATTC G^A[AT]TC
1765 GATTC
Van91I 474 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1614 CCACATTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BalI BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI