Restriction map for
pF1KE3526
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# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI AseI AsnI AspEI BbeI
BbrPI BcgI BclI BfrI BsaAI
BseAI BsiWI BsmI BspDI BspEI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EcoRI EcoRV
FbaI HincII HindII HindIII HpaI
KasI MfeI MluI MroI MseI
MunI NarI NdeI NheI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI SapI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspI StuI SwaI Tru9I XbaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 557 GGTACC G^GTACC
AcsI 1324 AAATTT [GA]^AATT[CT]
AflIII 1346 ACATGT A^C[GA][CT]GT
ApoI 1324 AAATTT [GA]^AATT[CT]
AscI 2071 GGCGCGCC GG^CGCGCC
Asp718I 557 GGTACC G^GTACC
AspI 147 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 431 TGCGCA TGC^GCA
AvrII 939 CCTAGG C^CTAGG
BbvCI 72 CCTCAGC CCTCAGC(-5/-2)
BciVI 1534 GTATCC GTATCC(6/5)
BglII 1409 AGATCT A^GATCT
BlnI 939 CCTAGG C^CTAGG
BsaBI 609 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1702 TCCGGT [AT]^CCGG[AT]
Bsp1407I 659 TGTACA T^GTACA
BspLU11I 1346 ACATGT A^CATGT
BsrDI 3039 CATTGC GCAATG(2/0)
BsrGI 659 TGTACA T^GTACA
DraIII 2153 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 3103 ATGCAT ATGCA^T
FspI 431 TGCGCA TGC^GCA
KpnI 557 GGTACC GGTAC^C
MamI 609 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 1148 GCGGCCGC GC^GGCCGC
NsiI 3103 ATGCAT ATGCA^T
PflFI 147 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2303 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 927 CCTGCAGG CCTGCA^GG
SfiI 879 GGCCACCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1988 CCCGGG CCC^GGG
Sse8387I 927 CCTGCAGG CCTGCA^GG
SspBI 659 TGTACA T^GTACA
TfiI 2777 GAATC G^A[AT]TC
Tsp509I 1325 AATT ^AATT
Tth111I 147 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1988 CCCGGG C^CCGGG
XmaI 1988 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 1563 CCTGAGG CC^T[ACGT]AGG
2526 CCTGAGG
Aor51HI 957 AGCGCT AGC^GCT
1394 AGCGCT
Asp700I 795 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1359 GAACCAGTTC
BamHI 990 GGATCC G^GATCC
1559 GGATCC
BbsI 2671 GAAGAC GAAGAC(2/6)
1090 GTCTTC
BpuAI 2671 GAAGAC GAAGAC(2/6)
1090 GTCTTC
BsmBI 1730 CGTCTC CGTCTC(1/5)
2830 GAGACG
BspHI 1355 TCATGA T^CATGA
3071 TCATGA
BssHII 622 GCGCGC G^CGCGC
2072 GCGCGC
BssSI 28 CACGAG CACGAG(-5/-1)
1122 CTCGTG
BstAPI 768 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1488 GCACTCACTGC
BstEII 1296 GGTCACC G^GT[ACGT]ACC
1724 GGTTACC
BstPI 1296 GGTCACC G^GT[ACGT]ACC
1724 GGTTACC
Bsu36I 1563 CCTGAGG CC^T[ACGT]AGG
2526 CCTGAGG
Eco47III 957 AGCGCT AGC^GCT
1394 AGCGCT
Eco81I 1563 CCTGAGG CC^T[ACGT]AGG
2526 CCTGAGG
EcoO65I 1296 GGTCACC G^GT[ACGT]ACC
1724 GGTTACC
FseI 1982 GGCCGGCC GGCCGG^CC
2080 GGCCGGCC
KspI 2261 CCGCGG CCGC^GG
2421 CCGCGG
MslI 651 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2916 CATCCAGATG
NcoI 650 CCATGG C^CATGG
1868 CCATGG
NspI 1346 ACATGT [GA]CATG^[CT]
1388 ACATGC
PaeR7I 715 CTCGAG C^TCGAG
1806 CTCGAG
RcaI 1355 TCATGA T^CATGA
3071 TCATGA
SacII 2261 CCGCGG CCGC^GG
2421 CCGCGG
XhoI 715 CTCGAG C^TCGAG
1806 CTCGAG
XmnI 795 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1359 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII