Restriction map for
pF1KSDA1735
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BanI
BbeI BbrPI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsiEI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 701 TCCGGA T^CCGGA
AfaI 1166 GTAC GT^AC
AlwNI 1158 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 893 GGACC G^G[AT]CC
AviII 935 TGCGCA TGC^GCA
BalI 951 TGGCCA TGG^CCA
BbsI 1399 GAAGAC GAAGAC(2/6)
BbvCI 961 CCTCAGC CCTCAGC(-5/-2)
BcnI 1098 CCGGG CC^[GC]GG
BpmI 193 CTGGAG CTGGAG(16/14)
BpuAI 1399 GAAGAC GAAGAC(2/6)
BsaBI 375 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 196 GAGACC GGTCTC(1/5)
BseAI 701 TCCGGA T^CCGGA
BsgI 571 CTGCAC GTGCAG(16/14)
BsmBI 1030 GAGACG CGTCTC(1/5)
BspEI 701 TCCGGA T^CCGGA
BspMI 902 ACCTGC ACCTGC(4/8)
BsrDI 836 GCAATG GCAATG(2/0)
DraI 1255 TTTAAA TTT^AAA
DraII 328 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 727 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1404 CCATGG C^C[GA][CT]GG
EarI 82 GAAGAG CTCTTC(1/4)
EcoO109I 328 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 270 GATATC GAT^ATC
FauI 109 CCCGC CCCGC(4/6)
FspI 935 TGCGCA TGC^GCA
HaeII 1013 AGCGCC [GA]GCGC^[CT]
Ksp632I 82 GAAGAG CTCTTC(1/4)
MaeII 1243 ACGT A^CGT
MamI 375 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 951 TGGCCA TGG^CCA
MroI 701 TCCGGA T^CCGGA
MscI 951 TGGCCA TGG^CCA
NciI 1098 CCGGG CC^[GC]GG
NcoI 1404 CCATGG C^CATGG
NspI 1020 GCATGT [GA]CATG^[CT]
PaeR7I 158 CTCGAG C^TCGAG
PshAI 9 GACGCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 214 CAGCTG CAG^CTG
RsaI 1166 GTAC GT^AC
SacI 1140 GAGCTC GAGCT^C
SspI 233 AATATT AAT^ATT
StuI 284 AGGCCT AGG^CCT
Tsp509I 1380 AATT ^AATT
XbaI 738 TCTAGA T^CTAGA
XhoI 158 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AgeI 1190 ACCGGT A^CCGGT
1286 ACCGGT
AspHI 1140 GAGCTC G[AT]GC[AT]^C
1174 GTGCTC
AvaI 158 CTCGAG C^[CT]CG[GA]G
361 CCCGAG
BamHI 301 GGATCC G^GATCC
1308 GGATCC
BanII 177 GAGCCC G[GA]GC[CT]^C
1140 GAGCTC
BcgI 651 GCAGAAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
906 GCAGCTTGTTCG
BsiHKAI 1140 GAGCTC G[AT]GC[AT]^C
1174 GTGCTC
BsoBI 158 CTCGAG C^[CT]CG[GA]G
361 CCCGAG
Cac8I 932 GCCTGC GC[ACGT]^[ACGT]GC
1016 GCCTGC
CfoI 936 GCGC GCG^C
1014 GCGC
EaeI 169 CGGCCA [CT]^GGCC[GA]
951 TGGCCA
EcoT14I 332 CCTTGG C^C[AT][AT]GG
1404 CCATGG
HgaI 9 GACGC GACGC(5/10)
1032 GACGC
HhaI 936 GCGC GCG^C
1014 GCGC
HinP1I 936 GCGC G^CGC
1014 GCGC
MslI 757 CACAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1345 CATGATGATG
MspA1I 110 CCGCTG C[AC]G^C[GT]G
214 CAGCTG
PflMI 579 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
726 CCACCAGGTGG
PinAI 1190 ACCGGT A^CCGGT
1286 ACCGGT
PstI 592 CTGCAG CTGCA^G
904 CTGCAG
SexAI 348 ACCAGGT A^CC[AT]GGT
728 ACCAGGT
SmlI 158 CTCGAG C^T[CT][GA]AG
1079 CTCAAG
StyI 332 CCTTGG C^C[AT][AT]GG
1404 CCATGG
TaqI 159 TCGA T^CGA
915 TCGA
TfiI 145 GATTC G^A[AT]TC
414 GAATC
TthHB8I 159 TCGA T^CGA
915 TCGA
Van91I 579 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
726 CCACCAGGTGG
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI BbvI BfaI
BmyI BsaJI BsaWI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cfr10I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI SfcI Tru9I TseI Tsp45I
TspRI XhoII