Restriction map for
pF1KSDA1733
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
BstBI BstUI Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1173 GTATAC GT^[AC][GT]AC
AccIII 179 TCCGGA T^CCGGA
AcyI 670 GGCGCC G[GA]^CG[CT]C
AflIII 404 ACACGT A^C[GA][CT]GT
AseI 135 ATTAAT AT^TAAT
AsnI 135 ATTAAT AT^TAAT
BamHI 612 GGATCC G^GATCC
BbeI 670 GGCGCC GGCGC^C
BcnI 108 CCGGG CC^[GC]GG
BfaI 532 CTAG C^TAG
BlpI 1395 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1395 GCTGAGC GC^T[ACGT]AGC
BsaHI 670 GGCGCC G[GA]^CG[CT]C
BsaWI 179 TCCGGA [AT]^CCGG[AT]
BseAI 179 TCCGGA T^CCGGA
BsmAI 567 GTCTC GTCTC(1/5)
BspEI 179 TCCGGA T^CCGGA
BsrBI 1348 GAGCGG CCGCTC(-3/-3)
Bst1107I 1173 GTATAC GTA^TAC
BstAPI 852 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1050 GGTCACC G^GT[ACGT]ACC
BstPI 1050 GGTCACC G^GT[ACGT]ACC
BstZ17I 1173 GTATAC GTA^TAC
CelII 1395 GCTGAGC GC^T[ACGT]AGC
DrdI 286 GACTCCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 213 CCTCGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1050 GGTCACC G^GT[ACGT]ACC
EcoT14I 364 CCTTGG C^C[AT][AT]GG
FauI 996 GCGGG CCCGC(4/6)
HaeII 670 GGCGCC [GA]GCGC^[CT]
Hin1I 670 GGCGCC G[GA]^CG[CT]C
KasI 670 GGCGCC G^GCGCC
MaeI 532 CTAG C^TAG
MaeIII 1051 GTCAC ^GT[ACGT]AC
MroI 179 TCCGGA T^CCGGA
NarI 670 GGCGCC GG^CGCC
NciI 108 CCGGG CC^[GC]GG
NdeI 623 CATATG CA^TATG
PflMI 661 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 135 ATTAAT AT^TAAT
SapI 451 GAAGAGC GCTCTTC(1/4)
SfoI 670 GGCGCC GGC^GCC
SmlI 98 CTCAAG C^T[CT][GA]AG
StyI 364 CCTTGG C^C[AT][AT]GG
TfiI 77 GATTC G^A[AT]TC
Tsp45I 1051 GTCAC ^GT[GC]AC
Tsp509I 683 AATT ^AATT
Van91I 661 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 210 GTAC GT^AC
864 GTAC
AvaI 921 CTCGGG C^[CT]CG[GA]G
1038 CTCGGG
AvaII 999 GGTCC G^G[AT]CC
1063 GGACC
BbsI 1156 GAAGAC GAAGAC(2/6)
1207 GAAGAC
BbvCI 250 GCTGAGG CCTCAGC(-5/-2)
1359 GCTGAGG
BpmI 555 CTCCAG CTGGAG(16/14)
1354 CTGGAG
BpuAI 1156 GAAGAC GAAGAC(2/6)
1207 GAAGAC
BsoBI 921 CTCGGG C^[CT]CG[GA]G
1038 CTCGGG
BspHI 734 TCATGA T^CATGA
1140 TCATGA
BsrFI 167 ACCGGC [GA]^CCGG[CT]
898 ACCGGC
CfoI 189 GCGC GCG^C
671 GCGC
Cfr10I 167 ACCGGC [GA]^CCGG[CT]
898 ACCGGC
DsaI 749 CCGTGG C^C[GA][CT]GG
873 CCACGG
HhaI 189 GCGC GCG^C
671 GCGC
HinP1I 189 GCGC G^CGC
671 GCGC
MaeII 406 ACGT A^CGT
1446 ACGT
PpuMI 998 GGGTCCC [GA]G^G[AT]CC[CT]
1062 AGGACCT
RcaI 734 TCATGA T^CATGA
1140 TCATGA
RsaI 210 GTAC GT^AC
864 GTAC
SfaNI 276 GATGC GCATC(5/9)
145 GCATC
XcmI 968 CCATGCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1298 CCAAGCGAGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
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AciI AluI AlwI AlwNI AspHI
BalI BanI BanII BbvI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI PvuII SacI
Sau3AI Sau96I ScrFI SfcI Tru9I
TseI TspRI XhoII