Restriction map for
pF1KSDA1721
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BglI BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BstBI BstEII
BstPI Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HgaI Hin1I KasI KpnI
KspI MamI MluI MroI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI SwaI
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 51 CGCG CG^CG
AflIII 3236 ACACGT A^C[GA][CT]GT
AocI 2995 CCTGAGG CC^T[ACGT]AGG
ApaI 19 GGGCCC GGGCC^C
AspHI 112 GAGCAC G[AT]GC[AT]^C
AspI 190 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1856 CTCGAG C^[CT]CG[GA]G
BalI 1433 TGGCCA TGG^CCA
BanI 69 GGCACC G^G[CT][GA]CC
BanII 19 GGGCCC G[GA]GC[CT]^C
BbvCI 2446 GCTGAGG CCTCAGC(-5/-2)
BcnI 1564 CCGGG CC^[GC]GG
BsiHKAI 112 GAGCAC G[AT]GC[AT]^C
BsmFI 1892 GTCCC GGGAC(10/14)
BsoBI 1856 CTCGAG C^[CT]CG[GA]G
BspHI 414 TCATGA T^CATGA
BsrDI 2442 CATTGC GCAATG(2/0)
BssSI 1370 CTCGTG CACGAG(-5/-1)
BstAPI 762 GCAAAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 51 CGCG CG^CG
BstXI 1355 CCAATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2995 CCTGAGG CC^T[ACGT]AGG
DrdI 1517 GACTCCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1433 TGGCCA [CT]^GGCC[GA]
Eco81I 2995 CCTGAGG CC^T[ACGT]AGG
HaeII 12 GGCGCT [GA]GCGC^[CT]
HapII 1564 CCGG C^CGG
HindIII 3227 AAGCTT A^AGCTT
HpaII 1564 CCGG C^CGG
MluNI 1433 TGGCCA TGG^CCA
MscI 1433 TGGCCA TGG^CCA
MspI 1564 CCGG C^CGG
MvnI 51 CGCG CG^CG
NciI 1564 CCGGG CC^[GC]GG
PaeR7I 1856 CTCGAG C^TCGAG
PflFI 190 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2090 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 580 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 40 CAGCTG CAG^CTG
RcaI 414 TCATGA T^CATGA
SapI 2713 GAAGAGC GCTCTTC(1/4)
ScaI 2936 AGTACT AGT^ACT
SpeI 178 ACTAGT A^CTAGT
SphI 105 GCATGC GCATG^C
StuI 3080 AGGCCT AGG^CCT
Tth111I 190 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1856 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1192 GTATAC GT^[AC][GT]AC
1475 GTATAC
AflII 2349 CTTAAG C^TTAAG
3160 CTTAAG
AlwNI 1802 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2546 CAGAGACTG
AseI 1732 ATTAAT AT^TAAT
2946 ATTAAT
AsnI 1732 ATTAAT AT^TAAT
2946 ATTAAT
AvaII 919 GGACC G^G[AT]CC
2091 GGACC
BbsI 577 GAAGAC GAAGAC(2/6)
657 GTCTTC
BclI 1425 TGATCA T^GATCA
1971 TGATCA
BfrI 2349 CTTAAG C^TTAAG
3160 CTTAAG
BlpI 37 GCTCAGC GC^T[ACGT]AGC
2188 GCTAAGC
BmyI 19 GGGCCC G[AGT]GC[ACT]^C
112 GAGCAC
Bpu1102I 37 GCTCAGC GC^T[ACGT]AGC
2188 GCTAAGC
BpuAI 577 GAAGAC GAAGAC(2/6)
657 GTCTTC
BsaI 1412 GAGACC GGTCTC(1/5)
3436 GGTCTC
BsgI 2110 GTGCAG GTGCAG(16/14)
2239 GTGCAG
BsmI 1445 GAATGC GAATGC(1/-1)
1713 GCATTC
Bsp1286I 19 GGGCCC G[AGT]GC[ACT]^C
112 GAGCAC
BspMI 2729 ACCTGC ACCTGC(4/8)
2759 ACCTGC
Bst1107I 1192 GTATAC GTA^TAC
1475 GTATAC
BstZ17I 1192 GTATAC GTA^TAC
1475 GTATAC
CelII 37 GCTCAGC GC^T[ACGT]AGC
2188 GCTAAGC
CfoI 13 GCGC GCG^C
484 GCGC
DraI 1923 TTTAAA TTT^AAA
3007 TTTAAA
EcoRI 541 GAATTC G^AATTC
2773 GAATTC
FauI 633 GCGGG CCCGC(4/6)
795 GCGGG
FbaI 1425 TGATCA T^GATCA
1971 TGATCA
HhaI 13 GCGC GCG^C
484 GCGC
HinP1I 13 GCGC G^CGC
484 GCGC
MaeII 2468 ACGT A^CGT
3238 ACGT
MfeI 392 CAATTG C^AATTG
2508 CAATTG
MspA1I 40 CAGCTG C[AC]G^C[GT]G
2852 CAGCGG
MunI 392 CAATTG C^AATTG
2508 CAATTG
NdeI 1205 CATATG CA^TATG
2284 CATATG
NspI 105 GCATGC [GA]CATG^[CT]
1281 ACATGC
PshBI 1732 ATTAAT AT^TAAT
2946 ATTAAT
SfaNI 3331 GATGC GCATC(5/9)
266 GCATC
SspI 1700 AATATT AAT^ATT
3263 AATATT
Tsp45I 2122 GTCAC ^GT[GC]AC
2898 GTCAC
XcmI 212 CCACAGCCATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
781 CCAACAGAGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BbvI BfaI BglII
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI HpaI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII XmnI