Restriction map for
pF1KSDA1716
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbrPI BbsI BbvCI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 769 GTCTAC GT^[AC][GT]AC
AlwNI 1670 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1303 AGCGCT AGC^GCT
ApaI 338 GGGCCC GGGCC^C
Asp700I 357 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 85 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2093 TGGCCA TGG^CCA
BciVI 2240 GTATCC GTATCC(6/5)
BfaI 1909 CTAG C^TAG
BglII 1316 AGATCT A^GATCT
BsaAI 1408 TACGTG [CT]AC^GT[GA]
BseRI 1702 GAGGAG GAGGAG(10/8)
BspMI 2234 GCAGGT ACCTGC(4/8)
BssHII 2146 GCGCGC G^CGCGC
DraIII 316 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1303 AGCGCT AGC^GCT
EcoNI 126 CCTACAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 1855 GTCAAC GT[CT]^[GA]AC
HindII 1855 GTCAAC GT[CT]^[GA]AC
MaeI 1909 CTAG C^TAG
MluNI 2093 TGGCCA TGG^CCA
MscI 2093 TGGCCA TGG^CCA
NaeI 1111 GCCGGC GCC^GGC
NcoI 1439 CCATGG C^CATGG
NgoMIV 1111 GCCGGC G^CCGGC
NspI 896 GCATGC [GA]CATG^[CT]
PflFI 85 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 315 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 2221 GAGCTC GAGCT^C
SapI 1641 GCTCTTC GCTCTTC(1/4)
SphI 896 GCATGC GCATG^C
TfiI 1311 GAATC G^A[AT]TC
Tth111I 85 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 315 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1160 CCAGCATCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 357 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BcgI 1812 CGACCTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
35 GCAGGCTTTTCG
BstYI 428 AGATCC [GA]^GATC[CT]
1316 AGATCT
EcoT14I 1232 CCAAGG C^C[AT][AT]GG
1439 CCATGG
MaeIII 879 GTCAC ^GT[ACGT]AC
2128 GTGAC
MflI 428 AGATCC [GA]^GATC[CT]
1316 AGATCT
MslI 798 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2097 CATCGCAGTG
PpuMI 263 AGGACCT [GA]G^G[AT]CC[CT]
2084 GGGACCC
SfiI 1839 GGCCCACGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2014 GGCCGCACCGGCC
SmaI 1776 CCCGGG CCC^GGG
2193 CCCGGG
SmlI 784 CTCAAG C^T[CT][GA]AG
1540 CTTGAG
StyI 1232 CCAAGG C^C[AT][AT]GG
1439 CCATGG
Tsp45I 879 GTCAC ^GT[GC]AC
2128 GTGAC
XhoII 428 AGATCC [GA]^GATC[CT]
1316 AGATCT
XmaCI 1776 CCCGGG C^CCGGG
2193 CCCGGG
XmaI 1776 CCCGGG C^CCGGG
2193 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StuI TaqI TseI
TspRI TthHB8I