Restriction map for
pF1KSDA1715
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tsp45I Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 420 TCCGGA T^CCGGA
AciI 451 CCGC CCGC(-3/-1)
AocI 934 CCTCAGG CC^T[ACGT]AGG
ApaI 555 GGGCCC GGGCC^C
Asp700I 521 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 749 CCCGAG C^[CT]CG[GA]G
BamHI 676 GGATCC G^GATCC
BanII 555 GGGCCC G[GA]GC[CT]^C
BbsI 707 GTCTTC GAAGAC(2/6)
BclI 254 TGATCA T^GATCA
BfaI 52 CTAG C^TAG
BmyI 555 GGGCCC G[AGT]GC[ACT]^C
BpuAI 707 GTCTTC GAAGAC(2/6)
BsaWI 420 TCCGGA [AT]^CCGG[AT]
BseAI 420 TCCGGA T^CCGGA
BsmAI 1180 GAGAC GTCTC(1/5)
BsmFI 1004 GTCCC GGGAC(10/14)
BsoBI 749 CCCGAG C^[CT]CG[GA]G
Bsp1286I 555 GGGCCC G[AGT]GC[ACT]^C
BspEI 420 TCCGGA T^CCGGA
BspMI 975 GCAGGT ACCTGC(4/8)
BsrDI 879 CATTGC GCAATG(2/0)
BstAPI 214 GCAAGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 676 GGATCC [GA]^GATC[CT]
Bsu36I 934 CCTCAGG CC^T[ACGT]AGG
Cac8I 541 GCAAGC GC[ACGT]^[ACGT]GC
DraI 333 TTTAAA TTT^AAA
EarI 969 GAAGAG CTCTTC(1/4)
Eco81I 934 CCTCAGG CC^T[ACGT]AGG
FbaI 254 TGATCA T^GATCA
HaeIII 556 GGCC GG^CC
HapII 421 CCGG C^CGG
HpaII 421 CCGG C^CGG
HphI 793 GGTGA GGTGA(8/7)
Ksp632I 969 GAAGAG CTCTTC(1/4)
MaeI 52 CTAG C^TAG
MflI 676 GGATCC [GA]^GATC[CT]
MroI 420 TCCGGA T^CCGGA
MslI 818 CACTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 467 CAGCTG C[AC]G^C[GT]G
MspI 421 CCGG C^CGG
NspI 169 ACATGC [GA]CATG^[CT]
PflMI 454 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 509 CTGCAG CTGCA^G
PvuII 467 CAGCTG CAG^CTG
SfaNI 1147 GCATC GCATC(5/9)
SspI 782 AATATT AAT^ATT
Van91I 454 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 51 TCTAGA T^CTAGA
XcmI 250 CCATTGATCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 676 GGATCC [GA]^GATC[CT]
XmnI 521 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwNI 692 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1223 CAGTTCCTG
AseI 135 ATTAAT AT^TAAT
399 ATTAAT
AsnI 135 ATTAAT AT^TAAT
399 ATTAAT
BbvI 466 GCAGC GCAGC(8/12)
511 GCAGC
BsaJI 552 CCAGGG C^C[ACGT][ACGT]GG
609 CCCTGG
BseRI 560 CTCCTC GAGGAG(10/8)
1168 GAGGAG
BsrI 574 CCAGT ACTGG(1/-1)
1041 CCAGT
BstF5I 712 CATCC GGATG(2/0)
327 GGATG
DraII 554 AGGGCCC [GA]G^G[ACGT]CC[CT]
555 GGGCCCT
EcoO109I 554 AGGGCCC [GA]G^G[ACGT]CC[CT]
555 GGGCCCT
FokI 712 CATCC GGATG(9/13)
327 GGATG
PleI 424 GACTC GAGTC(4/5)
1153 GACTC
PshBI 135 ATTAAT AT^TAAT
399 ATTAAT
TaqI 18 TCGA T^CGA
888 TCGA
TseI 466 GCAGC G^C[AT]GC
511 GCAGC
TthHB8I 18 TCGA T^CGA
888 TCGA
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BpmI BsiYI BslI BssKI BstNI
Cfr13I DdeI DpnII EcoRII Fnu4HI
HinfI ItaI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfcI TfiI Tru9I Tsp509I TspRI