Restriction map for
pF1KSDA1712
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BcnI BglI BglII
BlnI BpmI BsaAI BsaBI BsaHI
BsaJI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoT14I EcoT22I FauI FseI
FspI HaeII HapII HgaI Hin1I
HpaI HpaII KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AciI 51 CCGC CCGC(-3/-1)
AcsI 892 GAATTT [GA]^AATT[CT]
AfaI 1016 GTAC GT^AC
AflII 742 CTTAAG C^TTAAG
AlwNI 458 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 497 CCTCAGG CC^T[ACGT]AGG
ApoI 892 GAATTT [GA]^AATT[CT]
AvaII 945 GGACC G^G[AT]CC
BanII 190 GAGCTC G[GA]GC[CT]^C
BbvI 109 GCAGC GCAGC(8/12)
BciVI 564 GGATAC GTATCC(6/5)
BclI 246 TGATCA T^GATCA
BfaI 37 CTAG C^TAG
BfrI 742 CTTAAG C^TTAAG
BlpI 667 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 667 GCTGAGC GC^T[ACGT]AGC
BsaI 101 GAGACC GGTCTC(1/5)
BsgI 704 CTGCAC GTGCAG(16/14)
BsmAI 101 GAGAC GTCTC(1/5)
BsmI 727 GAATGC GAATGC(1/-1)
BspMI 485 GCAGGT ACCTGC(4/8)
BsrDI 440 GCAATG GCAATG(2/0)
BssKI 824 CCTGG ^CC[ACGT]GG
BstNI 824 CCTGG CC^[AT]GG
Bsu36I 497 CCTCAGG CC^T[ACGT]AGG
CelII 667 GCTGAGC GC^T[ACGT]AGC
CfoI 213 GCGC GCG^C
Cfr13I 945 GGACC G^G[ACGT]CC
DpnII 247 GATC ^GATC
Eco81I 497 CCTCAGG CC^T[ACGT]AGG
EcoNI 36 CCTAGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 824 CCTGG ^CC[AT]GG
EcoRV 475 GATATC GAT^ATC
FbaI 246 TGATCA T^GATCA
Fnu4HI 109 GCAGC GC^[ACGT]GC
HaeIII 983 GGCC GG^CC
HhaI 213 GCGC GCG^C
HinP1I 213 GCGC G^CGC
HincII 550 GTTGAC GT[CT]^[GA]AC
HindII 550 GTTGAC GT[CT]^[GA]AC
ItaI 109 GCAGC GC^[ACGT]GC
MaeI 37 CTAG C^TAG
MboI 247 GATC ^GATC
MvaI 824 CCTGG CC^[AT]GG
MwoI 48 GCTCCGCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 247 GATC ^GATC
NlaIII 942 CATG CATG^
PflMI 1098 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 1016 GTAC GT^AC
SacI 190 GAGCTC GAGCT^C
Sau3AI 247 GATC ^GATC
Sau96I 945 GGACC G^G[ACGT]CC
ScrFI 824 CCTGG CC^[ACGT]GG
SmlI 742 CTTAAG C^T[CT][GA]AG
TaqI 757 TCGA T^CGA
TseI 109 GCAGC G^C[AT]GC
TspRI 480 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 757 TCGA T^CGA
Van91I 1098 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AspHI 46 GTGCTC G[AT]GC[AT]^C
190 GAGCTC
BbsI 919 GAAGAC GAAGAC(2/6)
374 GTCTTC
BmyI 46 GTGCTC G[AGT]GC[ACT]^C
190 GAGCTC
BpuAI 919 GAAGAC GAAGAC(2/6)
374 GTCTTC
BsiHKAI 46 GTGCTC G[AT]GC[AT]^C
190 GAGCTC
BsiYI 36 CCTAGAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1098 CCAGAAAATGG
BslI 36 CCTAGAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1098 CCAGAAAATGG
Bsp1286I 46 GTGCTC G[AGT]GC[ACT]^C
190 GAGCTC
BstF5I 678 GGATG GGATG(2/0)
1112 GGATG
EarI 851 CTCTTC CTCTTC(1/4)
70 GAAGAG
FokI 678 GGATG GGATG(9/13)
1112 GGATG
HindIII 24 AAGCTT A^AGCTT
235 AAGCTT
Ksp632I 851 CTCTTC CTCTTC(1/4)
70 GAAGAG
NlaIV 32 GGAACC GG[ACGT]^[ACGT]CC
651 GGTTCC
PstI 455 CTGCAG CTGCA^G
1136 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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AluI Cac8I DdeI HinfI HphI
MaeIII MboII MnlI MseI SfaNI
SfcI TfiI Tru9I Tsp45I Tsp509I