Restriction map for
pF1KSDA1711
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccII AccIII AgeI AhdI
Aor51HI ApaI AscI Asp718I AspEI
AviII BamHI BbeI BbrPI BcgI
BcnI BglII BlpI Bpu1102I BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspMI BsrBI BsrFI
BssHII BstBI BstEII BstPI BstUI
CelII Cfr10I ClaI CpoI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I FseI FspI
HaeII HapII HindIII HpaII KasI
KpnI KspI MluI MroI MspI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 1786 GACGTC GACGT^C
AccI 829 GTATAC GT^[AC][GT]AC
AclI 2960 AACGTT AA^CGTT
AflII 2949 CTTAAG C^TTAAG
Alw44I 2844 GTGCAC G^TGCAC
AocI 2904 CCTCAGG CC^T[ACGT]AGG
ApaLI 2844 GTGCAC G^TGCAC
AspI 1831 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 353 CTCGGG C^[CT]CG[GA]G
AvrII 1186 CCTAGG C^CTAGG
BalI 2 TGGCCA TGG^CCA
BanII 1358 GAGCTC G[GA]GC[CT]^C
BfrI 2949 CTTAAG C^TTAAG
BglI 1736 GCCTGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1186 CCTAGG C^CTAGG
BpmI 2149 CTCCAG CTGGAG(16/14)
BsaAI 941 CACGTA [CT]AC^GT[GA]
BsaBI 2455 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1500 GTGCAG GTGCAG(16/14)
BsoBI 353 CTCGGG C^[CT]CG[GA]G
Bsp1407I 597 TGTACA T^GTACA
BspHI 2675 TCATGA T^CATGA
BsrDI 384 CATTGC GCAATG(2/0)
BsrGI 597 TGTACA T^GTACA
BssSI 1670 CTCGTG CACGAG(-5/-1)
Bst1107I 829 GTATAC GTA^TAC
BstAPI 1502 GCAGCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 639 CCAAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 829 GTATAC GTA^TAC
Bsu36I 2904 CCTCAGG CC^T[ACGT]AGG
CfoI 381 GCGC GCG^C
DrdI 1780 GACCTTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2 TGGCCA [CT]^GGCC[GA]
Eco81I 2904 CCTCAGG CC^T[ACGT]AGG
EcoRI 1453 GAATTC G^AATTC
EcoRV 1963 GATATC GAT^ATC
FauI 2871 GCGGG CCCGC(4/6)
HgaI 1276 GACGC GACGC(5/10)
HhaI 381 GCGC GCG^C
HinP1I 381 GCGC G^CGC
MaeIII 1778 GTGAC ^GT[ACGT]AC
MamI 2455 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1922 CAATTG C^AATTG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MunI 1922 CAATTG C^AATTG
PflFI 1831 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 212 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2960 AACGTT AA^CGTT
PstI 152 CTGCAG CTGCA^G
RcaI 2675 TCATGA T^CATGA
SacI 1358 GAGCTC GAGCT^C
SfcI 152 CTGCAG C^T[GA][CT]AG
SspBI 597 TGTACA T^GTACA
TaqI 2649 TCGA T^CGA
Tsp45I 1778 GTGAC ^GT[GC]AC
Tth111I 1831 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2649 TCGA T^CGA
XbaI 2098 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1276 GACGCC G[GA]^CG[CT]C
1786 GACGTC
AlwNI 2300 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
2876 CAGCTGCTG
AseI 222 ATTAAT AT^TAAT
1801 ATTAAT
AsnI 222 ATTAAT AT^TAAT
1801 ATTAAT
BanI 183 GGCACC G^G[CT][GA]CC
1917 GGTGCC
BbvCI 3065 CCTCAGC CCTCAGC(-5/-2)
1819 GCTGAGG
BciVI 785 GTATCC GTATCC(6/5)
1622 GTATCC
BclI 903 TGATCA T^GATCA
2250 TGATCA
BsaHI 1276 GACGCC G[GA]^CG[CT]C
1786 GACGTC
BsmI 540 GAATGC GAATGC(1/-1)
3001 GAATGC
DraII 642 AGGTCCT [GA]G^G[ACGT]CC[CT]
3076 AGGACCC
EcoO109I 642 AGGTCCT [GA]G^G[ACGT]CC[CT]
3076 AGGACCC
EcoT14I 639 CCAAGG C^C[AT][AT]GG
1186 CCTAGG
EcoT22I 2093 ATGCAT ATGCA^T
2120 ATGCAT
FbaI 903 TGATCA T^GATCA
2250 TGATCA
Hin1I 1276 GACGCC G[GA]^CG[CT]C
1786 GACGTC
HpaI 799 GTTAAC GTT^AAC
2610 GTTAAC
MslI 589 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
754 CACATCAGTG
MspA1I 662 CAGCTG C[AC]G^C[GT]G
2876 CAGCTG
NsiI 2093 ATGCAT ATGCA^T
2120 ATGCAT
PpuMI 642 AGGTCCT [GA]G^G[AT]CC[CT]
3076 AGGACCC
PshBI 222 ATTAAT AT^TAAT
1801 ATTAAT
PvuII 662 CAGCTG CAG^CTG
2876 CAGCTG
SapI 496 GCTCTTC GCTCTTC(1/4)
3020 GCTCTTC
SmlI 1390 CTCAAG C^T[CT][GA]AG
2949 CTTAAG
StyI 639 CCAAGG C^C[AT][AT]GG
1186 CCTAGG
XcmI 1938 CCATTTGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2914 CCAGTAGAAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BbsI BbvI BfaI BmyI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SspI
TfiI Tru9I TseI Tsp509I TspRI
XhoII XmnI