Restriction map for
pF1KSDA1696
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
Acc65I AccII AccIII AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BanI BanII BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I CfoI ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
FauI FbaI FseI FspI HaeII
HgaI HhaI HinP1I HindIII HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 543 GACGTC GACGT^C
AclI 891 AACGTT AA^CGTT
AcyI 543 GACGTC G[GA]^CG[CT]C
AflIII 1375 ACATGT A^C[GA][CT]GT
Asp700I 1483 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 502 GTGCTC G[AT]GC[AT]^C
BamHI 78 GGATCC G^GATCC
BbsI 360 GAAGAC GAAGAC(2/6)
BmyI 502 GTGCTC G[AGT]GC[ACT]^C
BpuAI 360 GAAGAC GAAGAC(2/6)
BsaHI 543 GACGTC G[GA]^CG[CT]C
BsaI 456 GAGACC GGTCTC(1/5)
BsiHKAI 502 GTGCTC G[AT]GC[AT]^C
BsmI 87 GAATGC GAATGC(1/-1)
Bsp1286I 502 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 963 TGTACA T^GTACA
BspLU11I 1375 ACATGT A^CATGT
BsrFI 1839 GCCGGC [GA]^CCGG[CT]
BsrGI 963 TGTACA T^GTACA
BstEII 474 GGTGACC G^GT[ACGT]ACC
BstPI 474 GGTGACC G^GT[ACGT]ACC
Cfr10I 1839 GCCGGC [GA]^CCGG[CT]
DraI 1587 TTTAAA TTT^AAA
DraII 1273 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 1704 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1273 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 474 GGTGACC G^GT[ACGT]ACC
EcoRI 837 GAATTC G^AATTC
EcoT22I 1650 ATGCAT ATGCA^T
Hin1I 543 GACGTC G[GA]^CG[CT]C
HincII 532 GTCAAC GT[CT]^[GA]AC
HindII 532 GTCAAC GT[CT]^[GA]AC
MfeI 1511 CAATTG C^AATTG
MunI 1511 CAATTG C^AATTG
NaeI 1839 GCCGGC GCC^GGC
NgoMIV 1839 GCCGGC G^CCGGC
NsiI 1650 ATGCAT ATGCA^T
PpuMI 1273 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 891 AACGTT AA^CGTT
SspBI 963 TGTACA T^GTACA
TfiI 1725 GATTC G^A[AT]TC
XbaI 1140 TCTAGA T^CTAGA
XmnI 1483 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1198 GTCTAC GT^[AC][GT]AC
1762 GTAGAC
AcsI 38 AAATTC [GA]^AATT[CT]
837 GAATTC
ApoI 38 AAATTC [GA]^AATT[CT]
837 GAATTC
BalI 1303 TGGCCA TGG^CCA
1353 TGGCCA
BbvCI 631 CCTCAGC CCTCAGC(-5/-2)
772 CCTCAGC
BsaWI 204 ACCGGA [AT]^CCGG[AT]
1194 TCCGGT
BspHI 111 TCATGA T^CATGA
1320 TCATGA
BspMI 1056 ACCTGC ACCTGC(4/8)
612 GCAGGT
BsrDI 227 CATTGC GCAATG(2/0)
465 CATTGC
BstAPI 418 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1334 GCAGCGTTTGC
BstXI 464 CCATTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
710 CCAAGCCTGTGG
BstYI 78 GGATCC [GA]^GATC[CT]
1442 GGATCT
DpnII 79 GATC ^GATC
1443 GATC
EaeI 1303 TGGCCA [CT]^GGCC[GA]
1353 TGGCCA
MboI 79 GATC ^GATC
1443 GATC
MflI 78 GGATCC [GA]^GATC[CT]
1442 GGATCT
MluNI 1303 TGGCCA TGG^CCA
1353 TGGCCA
MscI 1303 TGGCCA TGG^CCA
1353 TGGCCA
MspA1I 326 CAGCTG C[AC]G^C[GT]G
1720 CAGCTG
NcoI 899 CCATGG C^CATGG
1492 CCATGG
NdeII 79 GATC ^GATC
1443 GATC
NspI 1375 ACATGT [GA]CATG^[CT]
1436 GCATGT
PstI 329 CTGCAG CTGCA^G
610 CTGCAG
PvuII 326 CAGCTG CAG^CTG
1720 CAGCTG
RcaI 111 TCATGA T^CATGA
1320 TCATGA
Sau3AI 79 GATC ^GATC
1443 GATC
TaqI 705 TCGA T^CGA
1745 TCGA
TthHB8I 705 TCGA T^CGA
1745 TCGA
XhoII 78 GGATCC [GA]^GATC[CT]
1442 GGATCT
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BcnI BfaI BlpI
BpmI Bpu1102I BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstNI Cac8I CelII
Cfr13I DdeI DsaI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NlaIII NlaIV PflMI PleI
RsaI Sau96I ScrFI SfaNI SfcI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I