Restriction map for
pF1KSDA1691
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# NO CUT ENZYMES ( 121 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaBI BsaWI BseAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 658 GACGTC GACGT^C
AlwI 606 GGATC GGATC(4/5)
Aor51HI 1125 AGCGCT AGC^GCT
AspHI 229 GAGCAC G[AT]GC[AT]^C
BanI 21 GGCGCC G^G[CT][GA]CC
BbeI 21 GGCGCC GGCGC^C
BfaI 83 CTAG C^TAG
BglI 145 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 976 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 976 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 229 GAGCAC G[AT]GC[AT]^C
BsmBI 526 GAGACG CGTCTC(1/5)
Bsp1407I 1337 TGTACA T^GTACA
BsrGI 1337 TGTACA T^GTACA
BssHII 764 GCGCGC G^CGCGC
BssSI 1533 CACGAG CACGAG(-5/-1)
BstXI 836 CCAAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 606 GGATCT [GA]^GATC[CT]
CelII 976 GCTGAGC GC^T[ACGT]AGC
DpnII 607 GATC ^GATC
Eco47III 1125 AGCGCT AGC^GCT
EcoNI 557 CCTTTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 704 CCAAGG C^C[AT][AT]GG
KasI 21 GGCGCC G^GCGCC
MaeI 83 CTAG C^TAG
MboI 607 GATC ^GATC
MflI 606 GGATCT [GA]^GATC[CT]
NarI 21 GGCGCC GG^CGCC
NdeII 607 GATC ^GATC
NspI 1325 ACATGC [GA]CATG^[CT]
PflMI 1468 CCACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1019 AGGACCC [GA]G^G[AT]CC[CT]
SapI 183 GCTCTTC GCTCTTC(1/4)
Sau3AI 607 GATC ^GATC
SfoI 21 GGCGCC GGC^GCC
SspBI 1337 TGTACA T^GTACA
StyI 704 CCAAGG C^C[AT][AT]GG
TaqI 62 TCGA T^CGA
TfiI 311 GATTC G^A[AT]TC
Tsp509I 1438 AATT ^AATT
TthHB8I 62 TCGA T^CGA
Van91I 1468 CCACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 606 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AvaI 100 CCCGAG C^[CT]CG[GA]G
484 CCCGAG
BsaAI 829 TACGTG [CT]AC^GT[GA]
1117 TACGTG
BsaI 328 GAGACC GGTCTC(1/5)
1366 GAGACC
BsiEI 545 CGGCCG CG[GA][CT]^CG
1379 CGGCCG
BsoBI 100 CCCGAG C^[CT]CG[GA]G
484 CCCGAG
BspMI 1067 ACCTGC ACCTGC(4/8)
600 GCAGGT
BsrBI 1280 CCGCTC CCGCTC(-3/-3)
463 GAGCGG
EagI 545 CGGCCG C^GGCCG
1379 CGGCCG
EclXI 545 CGGCCG C^GGCCG
1379 CGGCCG
Eco52I 545 CGGCCG C^GGCCG
1379 CGGCCG
KspI 1291 CCGCGG CCGC^GG
1382 CCGCGG
SacII 1291 CCGCGG CCGC^GG
1382 CCGCGG
ScaI 851 AGTACT AGT^ACT
881 AGTACT
XcmI 374 CCAACCGCACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
704 CCAAGGGCATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaII BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NgoMIV NlaIII NlaIV PleI
PstI PvuII RsaI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I TspRI