Restriction map for
pF1KSDA1627
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HindIII HpaI HphI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 370 GTAGAC GT^[AC][GT]AC
AccII 57 CGCG CG^CG
AcyI 1025 GACGCC G[GA]^CG[CT]C
AflII 319 CTTAAG C^TTAAG
Asp700I 985 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 94 GGTGCC G^G[CT][GA]CC
BcnI 24 CCGGG CC^[GC]GG
BfrI 319 CTTAAG C^TTAAG
BglII 1291 AGATCT A^GATCT
BsaHI 1025 GACGCC G[GA]^CG[CT]C
BsmFI 905 GGGAC GGGAC(10/14)
Bsp1407I 1162 TGTACA T^GTACA
BsrBI 28 GAGCGG CCGCTC(-3/-3)
BsrGI 1162 TGTACA T^GTACA
BstUI 57 CGCG CG^CG
CfoI 58 GCGC GCG^C
EaeI 1269 TGGCCG [CT]^GGCC[GA]
EcoT14I 726 CCTTGG C^C[AT][AT]GG
EcoT22I 1121 ATGCAT ATGCA^T
HapII 24 CCGG C^CGG
HgaI 1025 GACGC GACGC(5/10)
HhaI 58 GCGC GCG^C
Hin1I 1025 GACGCC G[GA]^CG[CT]C
HinP1I 58 GCGC G^CGC
HincII 925 GTTGAC GT[CT]^[GA]AC
HindII 925 GTTGAC GT[CT]^[GA]AC
HpaII 24 CCGG C^CGG
MaeII 189 ACGT A^CGT
MspI 24 CCGG C^CGG
MvnI 57 CGCG CG^CG
NciI 24 CCGGG CC^[GC]GG
NsiI 1121 ATGCAT ATGCA^T
ScaI 310 AGTACT AGT^ACT
SfcI 980 CTGTAG C^T[GA][CT]AG
SmlI 319 CTTAAG C^T[CT][GA]AG
SspBI 1162 TGTACA T^GTACA
SspI 590 AATATT AAT^ATT
StyI 726 CCTTGG C^C[AT][AT]GG
XmnI 985 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 9 CCGC CCGC(-3/-1)
30 GCGG
AvaII 723 GGACC G^G[AT]CC
1081 GGACC
BanII 451 GAGCTC G[GA]GC[CT]^C
1232 GAGCTC
BbvI 60 GCAGC GCAGC(8/12)
673 GCAGC
BfaI 250 CTAG C^TAG
308 CTAG
BsmAI 211 GAGAC GTCTC(1/5)
1179 GAGAC
BstNI 805 CCTGG CC^[AT]GG
1063 CCAGG
DsaI 1272 CCGTGG C^C[GA][CT]GG
1332 CCGTGG
EcoRII 805 CCTGG ^CC[AT]GG
1063 CCAGG
HaeIII 1270 GGCC GG^CC
1330 GGCC
HinfI 304 GATTC G^A[ACGT]TC
564 GATTC
MaeI 250 CTAG C^TAG
308 CTAG
MaeIII 39 GTTAC ^GT[ACGT]AC
755 GTTAC
MslI 916 CATGCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1345 CACAGAGGTG
MvaI 805 CCTGG CC^[AT]GG
1063 CCAGG
NdeI 281 CATATG CA^TATG
1118 CATATG
SacI 451 GAGCTC GAGCT^C
1232 GAGCTC
SfaNI 608 GCATC GCATC(5/9)
1123 GCATC
TfiI 304 GATTC G^A[AT]TC
564 GATTC
TseI 60 GCAGC G^C[AT]GC
673 GCAGC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
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AcsI AfaI AluI AlwI ApoI
AspHI BbsI BmyI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstYI Cac8I
Cfr13I DdeI DpnII EarI Fnu4HI
FokI ItaI Ksp632I MboI MboII
MflI MnlI MseI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TaqI Tru9I Tsp509I TspRI
TthHB8I XhoII