Restriction map for
pF1KSDA1572
##################################
# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AciI 908 GCGG CCGC(-3/-1)
Alw44I 354 GTGCAC G^TGCAC
AlwI 1026 GGATC GGATC(4/5)
ApaLI 354 GTGCAC G^TGCAC
AspHI 354 GTGCAC G[AT]GC[AT]^C
AvaII 1257 GGTCC G^G[AT]CC
BanII 643 GAGCCC G[GA]GC[CT]^C
BbsI 508 GAAGAC GAAGAC(2/6)
BglII 15 AGATCT A^GATCT
BpmI 853 CTGGAG CTGGAG(16/14)
BpuAI 508 GAAGAC GAAGAC(2/6)
BsaI 176 GAGACC GGTCTC(1/5)
BsaJI 836 CCAGGG C^C[ACGT][ACGT]GG
BsiHKAI 354 GTGCAC G[AT]GC[AT]^C
BsmFI 135 GGGAC GGGAC(10/14)
BstYI 15 AGATCT [GA]^GATC[CT]
Cac8I 957 GCATGC GC[ACGT]^[ACGT]GC
DraI 873 TTTAAA TTT^AAA
DraIII 422 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 82 GAAGAG CTCTTC(1/4)
EcoRI 280 GAATTC G^AATTC
HapII 962 CCGG C^CGG
HpaII 962 CCGG C^CGG
Ksp632I 82 GAAGAG CTCTTC(1/4)
MfeI 247 CAATTG C^AATTG
MflI 15 AGATCT [GA]^GATC[CT]
MspA1I 1123 CAGCTG C[AC]G^C[GT]G
MspI 962 CCGG C^CGG
MunI 247 CAATTG C^AATTG
MwoI 1133 GCAAAAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PpuMI 1256 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 155 CTGCAG CTGCA^G
PvuII 1123 CAGCTG CAG^CTG
ScaI 1309 AGTACT AGT^ACT
SphI 957 GCATGC GCATG^C
SspI 781 AATATT AAT^ATT
StuI 801 AGGCCT AGG^CCT
XhoII 15 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 793 GTAGAC GT^[AC][GT]AC
899 GTCTAC
AflII 171 CTTAAG C^TTAAG
1296 CTTAAG
AlwNI 361 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1255 CAGGTCCTG
BfrI 171 CTTAAG C^TTAAG
1296 CTTAAG
BsmAI 176 GAGAC GTCTC(1/5)
243 GAGAC
Bsp1407I 1167 TGTACA T^GTACA
1204 TGTACA
BspMI 157 GCAGGT ACCTGC(4/8)
1267 GCAGGT
BsrGI 1167 TGTACA T^GTACA
1204 TGTACA
DraII 362 AGGCCCT [GA]G^G[ACGT]CC[CT]
1256 AGGTCCT
EcoO109I 362 AGGCCCT [GA]G^G[ACGT]CC[CT]
1256 AGGTCCT
NlaIV 160 GGTTCC GG[ACGT]^[ACGT]CC
469 GGCTCC
PleI 484 GACTC GAGTC(4/5)
1219 GACTC
SfcI 155 CTGCAG C^T[GA][CT]AG
805 CTACAG
SmlI 171 CTTAAG C^T[CT][GA]AG
1296 CTTAAG
SspBI 1167 TGTACA T^GTACA
1204 TGTACA
TaqI 786 TCGA T^CGA
942 TCGA
Tsp45I 848 GTCAC ^GT[GC]AC
1213 GTCAC
TthHB8I 786 TCGA T^CGA
942 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BmyI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MslI MvaI
NdeII NlaIII NspI RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I TspRI