Restriction map for
pF1KSDA1563
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AccIII AclI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspI AvaI
AviII BbrPI BcgI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoT22I FseI
FspI HpaI KspI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NotI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3429 GACGTC GACGT^C
Acc65I 523 GGTACC G^GTACC
AflIII 338 ACATGT A^C[GA][CT]GT
AgeI 526 ACCGGT A^CCGGT
AhdI 3975 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2935 CCTGAGG CC^T[ACGT]AGG
ApaI 3921 GGGCCC GGGCC^C
Asp718I 523 GGTACC G^GTACC
AspEI 3975 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1636 CCTAGG C^CTAGG
BciVI 1878 GGATAC GTATCC(6/5)
BclI 891 TGATCA T^GATCA
BcnI 2698 CCCGG CC^[GC]GG
BglI 3905 GCCAACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4742 AGATCT A^GATCT
BlnI 1636 CCTAGG C^CTAGG
BsaAI 4192 TACGTG [CT]AC^GT[GA]
BsaWI 526 ACCGGT [AT]^CCGG[AT]
Bsp1407I 648 TGTACA T^GTACA
BspLU11I 338 ACATGT A^CATGT
BsrFI 526 ACCGGT [GA]^CCGG[CT]
BsrGI 648 TGTACA T^GTACA
BssSI 2086 CACGAG CACGAG(-5/-1)
BstXI 2297 CCAGGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2935 CCTGAGG CC^T[ACGT]AGG
Cfr10I 526 ACCGGT [GA]^CCGG[CT]
DrdI 322 GACAATGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4790 CCATGG C^C[GA][CT]GG
Eam1105I 3975 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2935 CCTGAGG CC^T[ACGT]AGG
FauI 1149 CCCGC CCCGC(4/6)
FbaI 891 TGATCA T^GATCA
KpnI 523 GGTACC GGTAC^C
NciI 2698 CCCGG CC^[GC]GG
NcoI 4790 CCATGG C^CATGG
NheI 2215 GCTAGC G^CTAGC
NruI 4494 TCGCGA TCG^CGA
PinAI 526 ACCGGT A^CCGGT
PpuMI 1922 AGGACCC [GA]G^G[AT]CC[CT]
SacI 1298 GAGCTC GAGCT^C
SalI 2735 GTCGAC G^TCGAC
ScaI 1977 AGTACT AGT^ACT
SphI 2778 GCATGC GCATG^C
SspBI 648 TGTACA T^GTACA
XbaI 249 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 333 CGCG CG^CG
4495 CGCG
AlwNI 667 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
2596 CAGAAACTG
BbeI 3902 GGCGCC GGCGC^C
3986 GGCGCC
BbvCI 1166 CCTCAGC CCTCAGC(-5/-2)
4658 CCTCAGC
BlpI 638 GCTTAGC GC^T[ACGT]AGC
2404 GCTAAGC
Bpu1102I 638 GCTTAGC GC^T[ACGT]AGC
2404 GCTAAGC
BsaI 541 GGTCTC GGTCTC(1/5)
1188 GGTCTC
BspMI 1696 GCAGGT ACCTGC(4/8)
4214 GCAGGT
BsrDI 414 CATTGC GCAATG(2/0)
3277 GCAATG
BstEII 1702 GGTTACC G^GT[ACGT]ACC
3570 GGTTACC
BstPI 1702 GGTTACC G^GT[ACGT]ACC
3570 GGTTACC
BstUI 333 CGCG CG^CG
4495 CGCG
CelII 638 GCTTAGC GC^T[ACGT]AGC
2404 GCTAAGC
DraII 1922 AGGACCC [GA]G^G[ACGT]CC[CT]
3920 AGGGCCC
DraIII 2871 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3092 CACGCAGTG
EcoO109I 1922 AGGACCC [GA]G^G[ACGT]CC[CT]
3920 AGGGCCC
EcoO65I 1702 GGTTACC G^GT[ACGT]ACC
3570 GGTTACC
EcoRI 351 GAATTC G^AATTC
4051 GAATTC
HaeII 3902 GGCGCC [GA]GCGC^[CT]
3986 GGCGCC
HincII 1766 GTCAAC GT[CT]^[GA]AC
2735 GTCGAC
HindII 1766 GTCAAC GT[CT]^[GA]AC
2735 GTCGAC
KasI 3902 GGCGCC G^GCGCC
3986 GGCGCC
MaeII 3430 ACGT A^CGT
4193 ACGT
MvnI 333 CGCG CG^CG
4495 CGCG
NarI 3902 GGCGCC GG^CGCC
3986 GGCGCC
PflMI 2870 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3294 CCATTATGTGG
SfoI 3902 GGCGCC GGC^GCC
3986 GGCGCC
SspI 3958 AATATT AAT^ATT
4109 AATATT
Van91I 2870 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3294 CCATTATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflII AluI AlwI ApoI Asp700I
AspHI AvaII BalI BamHI BanI
BanII BbsI BbvI BfaI BfrI
BmyI BpmI BpuAI BsaBI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI EcoNI EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI