Restriction map for
pF1KSDA1559
##################################
# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BanI BanII
BbeI BbrPI BbvCI BbvI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI ItaI
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MspA1I MunI
MvnI MwoI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SmaI SmlI
SnaBI SphI Sse8387I SspI SwaI
TseI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AgeI 667 ACCGGT A^CCGGT
Alw44I 635 GTGCAC G^TGCAC
AlwNI 805 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 635 GTGCAC G^TGCAC
Asp700I 954 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 32 TGGCCA TGG^CCA
BamHI 69 GGATCC G^GATCC
BcgI 807 GCAACTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 269 CTCCAG CTGGAG(16/14)
BsaI 1263 GAGACC GGTCTC(1/5)
BsaWI 667 ACCGGT [AT]^CCGG[AT]
Bsp1407I 886 TGTACA T^GTACA
BsrFI 667 ACCGGT [GA]^CCGG[CT]
BsrGI 886 TGTACA T^GTACA
BssSI 1172 CTCGTG CACGAG(-5/-1)
BstYI 69 GGATCC [GA]^GATC[CT]
Cfr10I 667 ACCGGT [GA]^CCGG[CT]
DraII 135 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 6 CCATGG C^C[GA][CT]GG
EaeI 32 TGGCCA [CT]^GGCC[GA]
EarI 1279 GAAGAG CTCTTC(1/4)
EcoO109I 135 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 6 CCATGG C^C[AT][AT]GG
HapII 668 CCGG C^CGG
HincII 563 GTCAAC GT[CT]^[GA]AC
HindII 563 GTCAAC GT[CT]^[GA]AC
HpaII 668 CCGG C^CGG
Ksp632I 1279 GAAGAG CTCTTC(1/4)
MflI 69 GGATCC [GA]^GATC[CT]
MluNI 32 TGGCCA TGG^CCA
MscI 32 TGGCCA TGG^CCA
MspI 668 CCGG C^CGG
NcoI 6 CCATGG C^CATGG
NlaIII 7 CATG CATG^
PinAI 667 ACCGGT A^CCGGT
PleI 241 GAGTC GAGTC(4/5)
PpuMI 135 AGGACCT [GA]G^G[AT]CC[CT]
SgrAI 666 CACCGGTG C[GA]^CCGG[CT]G
SpeI 1146 ACTAGT A^CTAGT
SspBI 886 TGTACA T^GTACA
StyI 6 CCATGG C^C[AT][AT]GG
TfiI 1584 GATTC G^A[AT]TC
Tsp45I 16 GTGAC ^GT[GC]AC
XhoII 69 GGATCC [GA]^GATC[CT]
XmnI 954 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 70 GATCC GGATC(4/5)
69 GGATC
AspHI 635 GTGCAC G[AT]GC[AT]^C
715 GTGCTC
BfaI 133 CTAG C^TAG
1147 CTAG
BmyI 635 GTGCAC G[AGT]GC[ACT]^C
715 GTGCTC
BsaJI 6 CCATGG C^C[ACGT][ACGT]GG
193 CCTGGG
BsiHKAI 635 GTGCAC G[AT]GC[AT]^C
715 GTGCTC
BsmFI 83 GGGAC GGGAC(10/14)
214 GGGAC
Bsp1286I 635 GTGCAC G[AGT]GC[ACT]^C
715 GTGCTC
BssKI 66 CCTGG ^CC[ACGT]GG
193 CCTGG
BstNI 66 CCTGG CC^[AT]GG
193 CCTGG
Cac8I 618 GCAGGC GC[ACGT]^[ACGT]GC
633 GCGTGC
DpnII 70 GATC ^GATC
489 GATC
EcoRII 66 CCTGG ^CC[AT]GG
193 CCTGG
HinfI 241 GAGTC G^A[ACGT]TC
1584 GATTC
MaeI 133 CTAG C^TAG
1147 CTAG
MaeIII 16 GTGAC ^GT[ACGT]AC
1220 GTTAC
MboI 70 GATC ^GATC
489 GATC
MseI 326 TTAA T^TAA
1135 TTAA
MvaI 66 CCTGG CC^[AT]GG
193 CCTGG
NdeII 70 GATC ^GATC
489 GATC
Sau3AI 70 GATC ^GATC
489 GATC
ScrFI 66 CCTGG CC^[ACGT]GG
193 CCTGG
SfaNI 1323 GCATC GCATC(5/9)
1575 GCATC
SfcI 114 CTACAG C^T[GA][CT]AG
333 CTATAG
TaqI 648 TCGA T^CGA
816 TCGA
Tru9I 326 TTAA T^TAA
1135 TTAA
TthHB8I 648 TCGA T^CGA
816 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbsI BpuAI BsiYI BslI BsmAI
BsmI BsrI BstF5I Cfr13I DdeI
EcoRI FokI HaeIII HphI MboII
MnlI MslI NlaIV RsaI Sau96I
StuI Tsp509I TspRI