Restriction map for
pF1KSDA1443
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BcgI
BciVI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstYI
BstZ17I Cfr10I ClaI CpoI DraI
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FokI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MflI MluI
MluNI MroI MscI MslI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XhoII XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 379 GTCTAC GT^[AC][GT]AC
AlwI 1365 GATCC GGATC(4/5)
AocI 553 CCTGAGG CC^T[ACGT]AGG
AseI 801 ATTAAT AT^TAAT
AsnI 801 ATTAAT AT^TAAT
BclI 1634 TGATCA T^GATCA
BlpI 1467 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1467 GCTTAGC GC^T[ACGT]AGC
BsaI 97 GGTCTC GGTCTC(1/5)
BsaWI 1380 ACCGGA [AT]^CCGG[AT]
BsmI 634 GCATTC GAATGC(1/-1)
BsrBI 1326 CCGCTC CCGCTC(-3/-3)
BstXI 714 CCAGTCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 553 CCTGAGG CC^T[ACGT]AGG
CelII 1467 GCTTAGC GC^T[ACGT]AGC
Eco81I 553 CCTGAGG CC^T[ACGT]AGG
EcoRV 1438 GATATC GAT^ATC
FbaI 1634 TGATCA T^GATCA
HaeII 317 AGCGCC [GA]GCGC^[CT]
HgaI 165 GACGC GACGC(5/10)
MaeII 270 ACGT A^CGT
PaeR7I 365 CTCGAG C^TCGAG
PflMI 876 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 801 ATTAAT AT^TAAT
SfcI 151 CTACAG C^T[GA][CT]AG
SmaI 25 CCCGGG CCC^GGG
SmlI 365 CTCGAG C^T[CT][GA]AG
Van91I 876 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1536 TCTAGA T^CTAGA
XcmI 1091 CCAAAGAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 365 CTCGAG C^TCGAG
XmaCI 25 CCCGGG C^CCGGG
XmaI 25 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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BbvCI 1510 CCTCAGC CCTCAGC(-5/-2)
1519 GCTGAGG
BcnI 25 CCCGG CC^[GC]GG
26 CCGGG
BglI 21 GCCGCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
993 GCCCCAAGGGC
BspMI 206 ACCTGC ACCTGC(4/8)
1479 GCAGGT
BsrDI 252 CATTGC GCAATG(2/0)
197 GCAATG
BstAPI 247 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
799 GCATTAATTGC
DraII 501 AGGTCCT [GA]G^G[ACGT]CC[CT]
705 GGGTCCC
DraIII 950 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1142 CACGGCGTG
EcoNI 681 CCTCTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1394 CCTTGGAGAGG
EcoO109I 501 AGGTCCT [GA]G^G[ACGT]CC[CT]
705 GGGTCCC
EcoT14I 996 CCAAGG C^C[AT][AT]GG
1394 CCTTGG
HapII 26 CCGG C^CGG
1381 CCGG
HpaII 26 CCGG C^CGG
1381 CCGG
MfeI 794 CAATTG C^AATTG
1447 CAATTG
MspI 26 CCGG C^CGG
1381 CCGG
MunI 794 CAATTG C^AATTG
1447 CAATTG
NciI 25 CCCGG CC^[GC]GG
26 CCGGG
PpuMI 501 AGGTCCT [GA]G^G[AT]CC[CT]
705 GGGTCCC
PvuII 1099 CAGCTG CAG^CTG
1517 CAGCTG
SexAI 1020 ACCAGGT A^CC[AT]GGT
1272 ACCTGGT
StyI 996 CCAAGG C^C[AT][AT]GG
1394 CCTTGG
TaqI 366 TCGA T^CGA
1503 TCGA
TthHB8I 366 TCGA T^CGA
1503 TCGA
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BanI BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI Tru9I TseI Tsp45I
Tsp509I TspRI