Restriction map for
pF1KA1435
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AlwI AocI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BanII BbeI BbsI BbvCI BcgI
BciVI BfrI BlnI BpuAI BsaI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspMI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AcsI 280 GAATTT [GA]^AATT[CT]
AcyI 73 GACGCC G[GA]^CG[CT]C
AfaI 392 GTAC GT^AC
Alw44I 423 GTGCAC G^TGCAC
AlwNI 736 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 983 AGCGCT AGC^GCT
ApaLI 423 GTGCAC G^TGCAC
ApoI 280 GAATTT [GA]^AATT[CT]
AspHI 423 GTGCAC G[AT]GC[AT]^C
AspI 73 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 445 CTCGGG C^[CT]CG[GA]G
BalI 371 TGGCCA TGG^CCA
BanI 65 GGCACC G^G[CT][GA]CC
BbrPI 561 CACGTG CAC^GTG
BfaI 1075 CTAG C^TAG
BglI 76 GCCGTCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1172 AGATCT A^GATCT
BlpI 48 GCTGAGC GC^T[ACGT]AGC
BpmI 18 CTCCAG CTGGAG(16/14)
Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC
BsaAI 561 CACGTG [CT]AC^GT[GA]
BsaBI 1057 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 73 GACGCC G[GA]^CG[CT]C
BseRI 833 CTCCTC GAGGAG(10/8)
BsiHKAI 423 GTGCAC G[AT]GC[AT]^C
BsmBI 771 CGTCTC CGTCTC(1/5)
BsoBI 445 CTCGGG C^[CT]CG[GA]G
BspLU11I 1112 ACATGT A^CATGT
BsrBI 432 GAGCGG CCGCTC(-3/-3)
BsrDI 1125 CATTGC GCAATG(2/0)
BsrFI 394 ACCGGC [GA]^CCGG[CT]
BstAPI 941 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1143 GGTGACC G^GT[ACGT]ACC
BstPI 1143 GGTGACC G^GT[ACGT]ACC
BstYI 1172 AGATCT [GA]^GATC[CT]
CelII 48 GCTGAGC GC^T[ACGT]AGC
Cfr10I 394 ACCGGC [GA]^CCGG[CT]
DraII 620 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 375 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1118 CCATGG C^C[GA][CT]GG
Eco47III 983 AGCGCT AGC^GCT
EcoO109I 620 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1143 GGTGACC G^GT[ACGT]ACC
HaeII 983 AGCGCT [GA]GCGC^[CT]
Hin1I 73 GACGCC G[GA]^CG[CT]C
HindIII 547 AAGCTT A^AGCTT
MaeI 1075 CTAG C^TAG
MamI 1057 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1172 AGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MseI 302 TTAA T^TAA
MspA1I 631 CAGCGG C[AC]G^C[GT]G
NcoI 1118 CCATGG C^CATGG
PflFI 73 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1045 GACTC GAGTC(4/5)
PmaCI 561 CACGTG CAC^GTG
PmlI 561 CACGTG CAC^GTG
PpuMI 620 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 163 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 392 GTAC GT^AC
SmlI 987 CTCAAG C^T[CT][GA]AG
Tru9I 302 TTAA T^TAA
Tth111I 73 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1130 CCAGGGGACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1172 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 87 CGCG CG^CG
428 CGCG
AvaII 621 GGACC G^G[AT]CC
1154 GGACC
BclI 116 TGATCA T^GATCA
386 TGATCA
BmyI 64 GGGCAC G[AGT]GC[ACT]^C
423 GTGCAC
BsgI 733 GTGCAG GTGCAG(16/14)
972 GTGCAG
BsmAI 161 GAGAC GTCTC(1/5)
772 GTCTC
Bsp1286I 64 GGGCAC G[AGT]GC[ACT]^C
423 GTGCAC
BstUI 87 CGCG CG^CG
428 CGCG
CfoI 88 GCGC GCG^C
984 GCGC
EagI 5 CGGCCG C^GGCCG
83 CGGCCG
EarI 640 CTCTTC CTCTTC(1/4)
826 GAAGAG
EclXI 5 CGGCCG C^GGCCG
83 CGGCCG
Eco52I 5 CGGCCG C^GGCCG
83 CGGCCG
EcoT14I 101 CCAAGG C^C[AT][AT]GG
1118 CCATGG
FbaI 116 TGATCA T^GATCA
386 TGATCA
HhaI 88 GCGC GCG^C
984 GCGC
HinP1I 88 GCGC G^CGC
984 GCGC
Ksp632I 640 CTCTTC CTCTTC(1/4)
826 GAAGAG
MslI 666 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1113 CATGTCCATG
MvnI 87 CGCG CG^CG
428 CGCG
NspI 440 ACATGC [GA]CATG^[CT]
1112 ACATGT
PflMI 192 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
374 CCACAGAGTGG
PstI 939 CTGCAG CTGCA^G
1200 CTGCAG
SfaNI 652 GCATC GCATC(5/9)
665 GCATC
SfcI 939 CTGCAG C^T[GA][CT]AG
1200 CTGCAG
StyI 101 CCAAGG C^C[AT][AT]GG
1118 CCATGG
TaqI 59 TCGA T^CGA
1010 TCGA
TfiI 853 GATTC G^A[AT]TC
1033 GATTC
Tsp45I 79 GTCAC ^GT[GC]AC
1144 GTGAC
Tsp509I 281 AATT ^AATT
795 AATT
TthHB8I 59 TCGA T^CGA
1010 TCGA
Van91I 192 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
374 CCACAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AflIII AluI BbvI BcnI
BsaJI BsiEI BsiYI BslI BsmFI
BspHI BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RcaI
Sau3AI Sau96I ScrFI TseI TspRI