Restriction map for
pF1KSDA1431
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FbaI FseI FspI HpaI
KpnI KspI MamI MfeI MluI
MroI MspA1I MunI NaeI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AvaII 1145 GGTCC G^G[AT]CC
BalI 2172 TGGCCA TGG^CCA
BbeI 55 GGCGCC GGCGC^C
BbsI 2461 GTCTTC GAAGAC(2/6)
BbvI 729 GCAGC GCAGC(8/12)
BfaI 838 CTAG C^TAG
BglI 109 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2431 AGATCT A^GATCT
BlpI 814 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 814 GCTAAGC GC^T[ACGT]AGC
BpuAI 2461 GTCTTC GAAGAC(2/6)
BsaI 916 GAGACC GGTCTC(1/5)
BsaWI 1666 ACCGGA [AT]^CCGG[AT]
BsgI 2164 CTGCAC GTGCAG(16/14)
BspDI 1522 ATCGAT AT^CGAT
BstAPI 2024 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 814 GCTAAGC GC^T[ACGT]AGC
ClaI 1522 ATCGAT AT^CGAT
DraII 192 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 2172 TGGCCA [CT]^GGCC[GA]
EcoNI 1101 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 192 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1718 ATGCAT ATGCA^T
HaeII 55 GGCGCC [GA]GCGC^[CT]
HgaI 36 GACGC GACGC(5/10)
KasI 55 GGCGCC G^GCGCC
MaeI 838 CTAG C^TAG
MluNI 2172 TGGCCA TGG^CCA
MscI 2172 TGGCCA TGG^CCA
NarI 55 GGCGCC GG^CGCC
NsiI 1718 ATGCAT ATGCA^T
NspI 2521 ACATGC [GA]CATG^[CT]
PflMI 297 CCAGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 229 GCTCTTC GCTCTTC(1/4)
ScaI 632 AGTACT AGT^ACT
SfoI 55 GGCGCC GGC^GCC
TaqI 1523 TCGA T^CGA
TseI 729 GCAGC G^C[AT]GC
TthHB8I 1523 TCGA T^CGA
Van91I 297 CCAGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 36 GACGCC G[GA]^CG[CT]C
55 GGCGCC
AlwI 793 GGATC GGATC(4/5)
2556 GGATC
AspHI 206 GAGCTC G[AT]GC[AT]^C
1293 GAGCTC
BanI 55 GGCGCC G^G[CT][GA]CC
515 GGTGCC
BsaHI 36 GACGCC G[GA]^CG[CT]C
55 GGCGCC
BsiHKAI 206 GAGCTC G[AT]GC[AT]^C
1293 GAGCTC
BsrBI 40 CCGCTC CCGCTC(-3/-3)
179 GAGCGG
BstYI 2431 AGATCT [GA]^GATC[CT]
2556 GGATCT
EarI 230 CTCTTC CTCTTC(1/4)
1165 GAAGAG
EcoT14I 113 CCTTGG C^C[AT][AT]GG
481 CCTTGG
Hin1I 36 GACGCC G[GA]^CG[CT]C
55 GGCGCC
HincII 715 GTTGAC GT[CT]^[GA]AC
2517 GTCAAC
HindII 715 GTTGAC GT[CT]^[GA]AC
2517 GTCAAC
Ksp632I 230 CTCTTC CTCTTC(1/4)
1165 GAAGAG
MaeII 1119 ACGT A^CGT
2542 ACGT
MflI 2431 AGATCT [GA]^GATC[CT]
2556 GGATCT
SacI 206 GAGCTC GAGCT^C
1293 GAGCTC
SmlI 144 CTCAAG C^T[CT][GA]AG
559 CTCAAG
StuI 1364 AGGCCT AGG^CCT
2123 AGGCCT
StyI 113 CCTTGG C^C[AT][AT]GG
481 CCTTGG
XhoII 2431 AGATCT [GA]^GATC[CT]
2556 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI Asp700I AvaI BanII BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EcoRI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SspBI TfiI Tru9I Tsp45I Tsp509I
TspRI XmaCI XmaI XmnI