Restriction map for
pF1KSDA1412
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I Aor51HI ApaLI
AscI Asp718I AviII AvrII BbeI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HpaI KasI KpnI KspI
MluI MroI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I StuI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1435 GTAGAC GT^[AC][GT]AC
AhdI 1708 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 746 CCTCAGG CC^T[ACGT]AGG
ApaI 2626 GGGCCC GGGCC^C
Asp700I 1070 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1708 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3569 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 791 CCCGGG C^[CT]CG[GA]G
BanI 3153 GGCACC G^G[CT][GA]CC
BanII 2626 GGGCCC G[GA]GC[CT]^C
BbrPI 2303 CACGTG CAC^GTG
BbsI 163 GAAGAC GAAGAC(2/6)
BbvCI 1447 GCTGAGG CCTCAGC(-5/-2)
BpuAI 163 GAAGAC GAAGAC(2/6)
BsaAI 2303 CACGTG [CT]AC^GT[GA]
BsmBI 355 CGTCTC CGTCTC(1/5)
BsoBI 791 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2991 TGTACA T^GTACA
BspHI 2705 TCATGA T^CATGA
BspLU11I 2766 ACATGT A^CATGT
BspMI 3594 GCAGGT ACCTGC(4/8)
BsrGI 2991 TGTACA T^GTACA
BstAPI 1073 GCACTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 746 CCTCAGG CC^T[ACGT]AGG
CfoI 556 GCGC GCG^C
DrdI 2194 GACAAAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3157 CCACGG C^C[GA][CT]GG
Eam1105I 1708 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 746 CCTCAGG CC^T[ACGT]AGG
EcoNI 537 CCTGATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 555 GGCGCT [GA]GCGC^[CT]
HhaI 556 GCGC GCG^C
HinP1I 556 GCGC G^CGC
HincII 3181 GTCAAC GT[CT]^[GA]AC
HindII 3181 GTCAAC GT[CT]^[GA]AC
MfeI 2693 CAATTG C^AATTG
MunI 2693 CAATTG C^AATTG
PflFI 3569 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2303 CACGTG CAC^GTG
PmlI 2303 CACGTG CAC^GTG
RcaI 2705 TCATGA T^CATGA
ScaI 3518 AGTACT AGT^ACT
SmaI 791 CCCGGG CCC^GGG
SmlI 1734 CTCAAG C^T[CT][GA]AG
SspBI 2991 TGTACA T^GTACA
Tth111I 3569 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 791 CCCGGG C^CCGGG
XmaI 791 CCCGGG C^CCGGG
XmnI 1070 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 436 GACGCC G[GA]^CG[CT]C
3730 GGCGTC
AflIII 2304 ACGTGT A^C[GA][CT]GT
2766 ACATGT
AseI 2023 ATTAAT AT^TAAT
3073 ATTAAT
AsnI 2023 ATTAAT AT^TAAT
3073 ATTAAT
BsaBI 343 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3037 GATGAGTATC
BsaHI 436 GACGCC G[GA]^CG[CT]C
3730 GGCGTC
BsrFI 3150 GCCGGC [GA]^CCGG[CT]
3552 GCCGGC
BssSI 3032 CACGAG CACGAG(-5/-1)
2749 CTCGTG
Cfr10I 3150 GCCGGC [GA]^CCGG[CT]
3552 GCCGGC
FauI 680 GCGGG CCCGC(4/6)
1121 GCGGG
Hin1I 436 GACGCC G[GA]^CG[CT]C
3730 GGCGTC
MamI 343 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3037 GATGAGTATC
NaeI 3150 GCCGGC GCC^GGC
3552 GCCGGC
NgoMIV 3150 GCCGGC G^CCGGC
3552 GCCGGC
NspI 1692 ACATGC [GA]CATG^[CT]
2766 ACATGT
PshBI 2023 ATTAAT AT^TAAT
3073 ATTAAT
PstI 2036 CTGCAG CTGCA^G
2964 CTGCAG
PvuII 2033 CAGCTG CAG^CTG
4066 CAGCTG
SspI 511 AATATT AAT^ATT
1471 AATATT
XcmI 42 CCAACCTCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2850 CCATGACATTGATGG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BamHI BbvI BciVI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SpeI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII