Restriction map for
pF1KSDA1394
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# NO CUT ENZYMES ( 78 enzymes) #
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AatII AclI AcsI AflII AflIII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AviII BbvCI BciVI BclI BfrI
BglII BsaBI BsiWI BsmI Bsp1407I
BspLU11I BsrBI BsrGI Bst1107I BstBI
BstZ17I Bsu36I CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoRI EcoT22I
FbaI FspI HindIII HpaI MamI
MfeI MluI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
ScaI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tru9I Tsp509I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 2049 GGTACC G^GTACC
AccI 238 GTCTAC GT^[AC][GT]AC
AccIII 285 TCCGGA T^CCGGA
Alw44I 2041 GTGCAC G^TGCAC
ApaLI 2041 GTGCAC G^TGCAC
Asp718I 2049 GGTACC G^GTACC
AspI 541 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 792 CCTAGG C^CTAGG
BalI 2616 TGGCCA TGG^CCA
BbrPI 2606 CACGTG CAC^GTG
BbsI 2434 GTCTTC GAAGAC(2/6)
BcgI 1631 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 792 CCTAGG C^CTAGG
BpuAI 2434 GTCTTC GAAGAC(2/6)
BsaAI 2606 CACGTG [CT]AC^GT[GA]
BseAI 285 TCCGGA T^CCGGA
BspDI 2851 ATCGAT AT^CGAT
BspEI 285 TCCGGA T^CCGGA
BspHI 2066 TCATGA T^CATGA
BsrDI 2204 GCAATG GCAATG(2/0)
BstEII 339 GGTGACC G^GT[ACGT]ACC
BstPI 339 GGTGACC G^GT[ACGT]ACC
ClaI 2851 ATCGAT AT^CGAT
EcoO65I 339 GGTGACC G^GT[ACGT]ACC
EcoRV 889 GATATC GAT^ATC
FseI 1338 GGCCGGCC GGCCGG^CC
HincII 2542 GTTGAC GT[CT]^[GA]AC
HindII 2542 GTTGAC GT[CT]^[GA]AC
KpnI 2049 GGTACC GGTAC^C
MluNI 2616 TGGCCA TGG^CCA
MroI 285 TCCGGA T^CCGGA
MscI 2616 TGGCCA TGG^CCA
NcoI 1959 CCATGG C^CATGG
PflFI 541 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2606 CACGTG CAC^GTG
PmlI 2606 CACGTG CAC^GTG
RcaI 2066 TCATGA T^CATGA
SapI 1926 GAAGAGC GCTCTTC(1/4)
SfiI 1308 GGCCGCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2345 GCATGC GCATG^C
StuI 579 AGGCCT AGG^CCT
TfiI 1109 GAATC G^A[AT]TC
Tth111I 541 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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ApaI 117 GGGCCC GGGCC^C
2857 GGGCCC
BsaI 1555 GAGACC GGTCTC(1/5)
2686 GAGACC
BsaWI 285 TCCGGA [AT]^CCGG[AT]
1754 ACCGGA
BssSI 1681 CTCGTG CACGAG(-5/-1)
2612 CTCGTG
BstAPI 1944 GCACCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2694 GCAGGCCCTGC
EarI 112 GAAGAG CTCTTC(1/4)
1926 GAAGAG
Ksp632I 112 GAAGAG CTCTTC(1/4)
1926 GAAGAG
MaeIII 340 GTGAC ^GT[ACGT]AC
629 GTGAC
MslI 1954 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2704 CACGACCGTG
NspI 416 ACATGC [GA]CATG^[CT]
2345 GCATGC
PpuMI 69 GGGTCCC [GA]G^G[AT]CC[CT]
101 AGGACCT
SacI 2446 GAGCTC GAGCT^C
2530 GAGCTC
SexAI 2045 ACCAGGT A^CC[AT]GGT
2255 ACCTGGT
SmlI 1810 CTCAAG C^T[CT][GA]AG
2449 CTCAAG
Tsp45I 340 GTGAC ^GT[GC]AC
629 GTGAC
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BamHI BanI BanII BbeI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KspI MaeI
MaeII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI SacII Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SfoI
SmaI Sse8387I StyI TaqI TseI
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI