Restriction map for
pF1KSDA1391
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII Acc65I AccI AclI AcyI
AgeI AhdI AscI Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BcgI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsiEI BsiWI BsmBI
BspDI BspHI BspMI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FseI
FspI HgaI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 81 CGCG CG^CG
AccIII 2995 TCCGGA T^CCGGA
Alw44I 2040 GTGCAC G^TGCAC
Aor51HI 136 AGCGCT AGC^GCT
ApaI 2024 GGGCCC GGGCC^C
ApaLI 2040 GTGCAC G^TGCAC
AseI 2695 ATTAAT AT^TAAT
AsnI 2695 ATTAAT AT^TAAT
AspI 1843 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2365 CACGTG CAC^GTG
BbvCI 3476 CCTCAGC CCTCAGC(-5/-2)
BciVI 2476 GGATAC GTATCC(6/5)
BclI 1353 TGATCA T^GATCA
BsaAI 2365 CACGTG [CT]AC^GT[GA]
BsaWI 2995 TCCGGA [AT]^CCGG[AT]
BseAI 2995 TCCGGA T^CCGGA
Bsp1407I 1991 TGTACA T^GTACA
BspEI 2995 TCCGGA T^CCGGA
BsrBI 304 GAGCGG CCGCTC(-3/-3)
BsrDI 2978 GCAATG GCAATG(2/0)
BsrGI 1991 TGTACA T^GTACA
BstUI 81 CGCG CG^CG
DraIII 2887 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 63 GACAGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3059 CCATGG C^C[GA][CT]GG
Eco47III 136 AGCGCT AGC^GCT
EcoT22I 3079 ATGCAT ATGCA^T
FbaI 1353 TGATCA T^GATCA
HincII 208 GTTGAC GT[CT]^[GA]AC
HindII 208 GTTGAC GT[CT]^[GA]AC
MaeII 2366 ACGT A^CGT
MroI 2995 TCCGGA T^CCGGA
MvnI 81 CGCG CG^CG
NcoI 3059 CCATGG C^CATGG
NdeI 788 CATATG CA^TATG
NsiI 3079 ATGCAT ATGCA^T
PflFI 1843 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2365 CACGTG CAC^GTG
PmlI 2365 CACGTG CAC^GTG
PshBI 2695 ATTAAT AT^TAAT
SacI 3444 GAGCTC GAGCT^C
SgrAI 2098 CACCGGCG C[GA]^CCGG[CT]G
SspBI 1991 TGTACA T^GTACA
Tth111I 1843 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 1258 GTAC GT^AC
1992 GTAC
AflII 3282 CTTAAG C^TTAAG
3397 CTTAAG
AflIII 680 ACATGT A^C[GA][CT]GT
2263 ACATGT
AlwNI 952 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
2288 CAGGCACTG
AvaI 899 CCCGAG C^[CT]CG[GA]G
1882 CCCGAG
BfrI 3282 CTTAAG C^TTAAG
3397 CTTAAG
BglII 994 AGATCT A^GATCT
1344 AGATCT
BsaI 1362 GGTCTC GGTCTC(1/5)
2626 GGTCTC
BsoBI 899 CCCGAG C^[CT]CG[GA]G
1882 CCCGAG
BspLU11I 680 ACATGT A^CATGT
2263 ACATGT
BsrFI 1974 GCCGGT [GA]^CCGG[CT]
2099 ACCGGC
BstAPI 2068 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2071 GCAAATGCTGC
BstXI 932 CCAGCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2399 CCATTTCTGTGG
Cfr10I 1974 GCCGGT [GA]^CCGG[CT]
2099 ACCGGC
DraI 982 TTTAAA TTT^AAA
2269 TTTAAA
EcoRI 651 GAATTC G^AATTC
1242 GAATTC
FauI 82 GCGGG CCCGC(4/6)
2010 GCGGG
HindIII 2140 AAGCTT A^AGCTT
2340 AAGCTT
PpuMI 1170 AGGACCT [GA]G^G[AT]CC[CT]
3204 AGGACCC
RsaI 1258 GTAC GT^AC
1992 GTAC
SapI 579 GAAGAGC GCTCTTC(1/4)
3446 GCTCTTC
SspI 1105 AATATT AAT^ATT
1318 AATATT
StuI 288 AGGCCT AGG^CCT
1893 AGGCCT
XbaI 555 TCTAGA T^CTAGA
3495 TCTAGA
XcmI 1130 CCAAACCTGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1543 CCAAGTATTCTTTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
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AciI AcsI AluI AlwI AocI
ApoI Asp700I AspHI AvaII BalI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI