Restriction map for
pF1KSDA1387
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AvaI AviII AvrII
BalI BbeI BbvCI BclI BfrI
BglI BlnI BpmI BsaHI BsaI
BsaWI BseAI BsgI BsiWI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I FauI FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 11 CGCG CG^CG
AflIII 406 ACGTGT A^C[GA][CT]GT
AspI 2531 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2404 GGATCC G^GATCC
BanII 100 GAGCTC G[GA]GC[CT]^C
BbrPI 405 CACGTG CAC^GTG
BcgI 1984 GCACTTGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 71 CCGGG CC^[GC]GG
BglII 275 AGATCT A^GATCT
BsiEI 60 CGACCG CG[GA][CT]^CG
BsmBI 78 CGTCTC CGTCTC(1/5)
BspDI 1992 ATCGAT AT^CGAT
BspMI 398 ACCTGC ACCTGC(4/8)
BsrDI 51 GCAATG GCAATG(2/0)
BsrFI 47 ACCGGC [GA]^CCGG[CT]
BstUI 11 CGCG CG^CG
BstXI 86 CCACTTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1298 GCGC GCG^C
Cfr10I 47 ACCGGC [GA]^CCGG[CT]
ClaI 1992 ATCGAT AT^CGAT
DraI 1476 TTTAAA TTT^AAA
DraII 332 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 332 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1939 GATATC GAT^ATC
HaeII 1297 GGCGCT [GA]GCGC^[CT]
HaeIII 1758 GGCC GG^CC
HhaI 1298 GCGC GCG^C
HinP1I 1298 GCGC G^CGC
HindIII 539 AAGCTT A^AGCTT
MvnI 11 CGCG CG^CG
NciI 71 CCGGG CC^[GC]GG
PflFI 2531 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 405 CACGTG CAC^GTG
PmlI 405 CACGTG CAC^GTG
PpuMI 332 AGGACCT [GA]G^G[AT]CC[CT]
SacI 100 GAGCTC GAGCT^C
SpeI 382 ACTAGT A^CTAGT
TaqI 1993 TCGA T^CGA
Tsp45I 325 GTCAC ^GT[GC]AC
Tth111I 2531 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1993 TCGA T^CGA
XcmI 2450 CCAAGGGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AlwNI 764 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
2062 CAGAAACTG
AseI 204 ATTAAT AT^TAAT
1246 ATTAAT
AsnI 204 ATTAAT AT^TAAT
1246 ATTAAT
Asp700I 1999 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2281 GAAAACCTTC
AspHI 100 GAGCTC G[AT]GC[AT]^C
454 GTGCTC
AvaII 333 GGACC G^G[AT]CC
1191 GGTCC
BanI 67 GGCACC G^G[CT][GA]CC
2187 GGCACC
BciVI 6 GGATAC GTATCC(6/5)
198 GGATAC
BlpI 697 GCTCAGC GC^T[ACGT]AGC
1216 GCTCAGC
Bpu1102I 697 GCTCAGC GC^T[ACGT]AGC
1216 GCTCAGC
BsaAI 91 TACGTG [CT]AC^GT[GA]
405 CACGTG
BsaBI 1276 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1606 GATAATTATC
BsiHKAI 100 GAGCTC G[AT]GC[AT]^C
454 GTGCTC
BstNI 2305 CCTGG CC^[AT]GG
2522 CCAGG
BstYI 275 AGATCT [GA]^GATC[CT]
2404 GGATCC
CelII 697 GCTCAGC GC^T[ACGT]AGC
1216 GCTCAGC
Cfr13I 333 GGACC G^G[ACGT]CC
1191 GGTCC
EcoNI 609 CCTAAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1285 CCTGAGCTAGG
EcoRII 2305 CCTGG ^CC[AT]GG
2522 CCAGG
EcoT22I 1058 ATGCAT ATGCA^T
1411 ATGCAT
HapII 48 CCGG C^CGG
71 CCGG
HpaII 48 CCGG C^CGG
71 CCGG
HphI 463 TCACC GGTGA(8/7)
1826 TCACC
MamI 1276 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1606 GATAATTATC
MflI 275 AGATCT [GA]^GATC[CT]
2404 GGATCC
MslI 87 CACTTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
577 CATTTGTATG
MspI 48 CCGG C^CGG
71 CCGG
MvaI 2305 CCTGG CC^[AT]GG
2522 CCAGG
NsiI 1058 ATGCAT ATGCA^T
1411 ATGCAT
PshBI 204 ATTAAT AT^TAAT
1246 ATTAAT
Sau96I 333 GGACC G^G[ACGT]CC
1191 GGTCC
SmlI 103 CTCAAG C^T[CT][GA]AG
1559 CTCAAG
XhoII 275 AGATCT [GA]^GATC[CT]
2404 GGATCC
XmnI 1999 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2281 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbsI BbvI BfaI BmyI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I Cac8I DdeI DpnII EarI
EcoRI EcoT14I Fnu4HI FokI HinfI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MwoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp509I TspRI