Restriction map for
pF1KSDA1382
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BclI BfaI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I BspDI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HpaI KasI KpnI KspI MaeI
MluI MspA1I MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 1181 TCCGGA T^CCGGA
AcyI 1232 GGCGTC G[GA]^CG[CT]C
AflIII 1056 ACATGT A^C[GA][CT]GT
AlwNI 446 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1069 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1512 TGGCCA TGG^CCA
BciVI 1036 GGATAC GTATCC(6/5)
BcnI 36 CCCGG CC^[GC]GG
BpmI 1505 CTGGAG CTGGAG(16/14)
BsaBI 127 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1232 GGCGTC G[GA]^CG[CT]C
BseAI 1181 TCCGGA T^CCGGA
BsiYI 1504 CCTGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1504 CCTGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 1241 GTCTC GTCTC(1/5)
Bsp1407I 1407 TGTACA T^GTACA
BspEI 1181 TCCGGA T^CCGGA
BspLU11I 1056 ACATGT A^CATGT
BspMI 804 ACCTGC ACCTGC(4/8)
BsrDI 242 GCAATG GCAATG(2/0)
BsrGI 1407 TGTACA T^GTACA
BstYI 132 AGATCC [GA]^GATC[CT]
DdeI 238 CTGAG C^T[ACGT]AG
DrdI 324 GACATTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 284 CCATGG C^C[GA][CT]GG
EaeI 1512 TGGCCA [CT]^GGCC[GA]
EcoRV 1306 GATATC GAT^ATC
HgaI 1233 GCGTC GACGC(5/10)
Hin1I 1232 GGCGTC G[GA]^CG[CT]C
HindIII 439 AAGCTT A^AGCTT
MaeII 821 ACGT A^CGT
MamI 127 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 406 CAATTG C^AATTG
MflI 132 AGATCC [GA]^GATC[CT]
MluNI 1512 TGGCCA TGG^CCA
MroI 1181 TCCGGA T^CCGGA
MscI 1512 TGGCCA TGG^CCA
MslI 1245 CATTACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 406 CAATTG C^AATTG
MwoI 1327 GCATCTGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 36 CCCGG CC^[GC]GG
NcoI 284 CCATGG C^CATGG
NlaIV 726 GGTTCC GG[ACGT]^[ACGT]CC
NspI 1056 ACATGT [GA]CATG^[CT]
PstI 1331 CTGCAG CTGCA^G
SspBI 1407 TGTACA T^GTACA
SspI 336 AATATT AAT^ATT
TaqI 163 TCGA T^CGA
TthHB8I 163 TCGA T^CGA
XhoII 132 AGATCC [GA]^GATC[CT]
XmnI 1069 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AciI 954 CCGC CCGC(-3/-1)
1025 CCGC
BbsI 43 GAAGAC GAAGAC(2/6)
369 GAAGAC
BpuAI 43 GAAGAC GAAGAC(2/6)
369 GAAGAC
BsaWI 1181 TCCGGA [AT]^CCGG[AT]
1459 ACCGGA
BsmFI 18 GGGAC GGGAC(10/14)
1291 GTCCC
EarI 496 CTCTTC CTCTTC(1/4)
1086 CTCTTC
EcoT14I 267 CCTTGG C^C[AT][AT]GG
284 CCATGG
Ksp632I 496 CTCTTC CTCTTC(1/4)
1086 CTCTTC
PleI 835 GACTC GAGTC(4/5)
1063 GAGTC
SfcI 57 CTACAG C^T[GA][CT]AG
1331 CTGCAG
StyI 267 CCTTGG C^C[AT][AT]GG
284 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BsaJI BsrI BssKI BstF5I
BstNI Cac8I DpnII EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MseI MspI MvaI NdeII
NlaIII RsaI Sau3AI ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI