Restriction map for
pF1KSDA1363
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaI
BsiEI BsiWI BsoBI BspDI BspLU11I
BssHII BssSI Bst1107I BstBI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI KasI KpnI KspI MamI
MfeI MflI MluI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 55 GTCTAC GT^[AC][GT]AC
AccII 219 CGCG CG^CG
AccIII 1181 TCCGGA T^CCGGA
AcyI 109 GACGCC G[GA]^CG[CT]C
AflIII 1030 ACGTGT A^C[GA][CT]GT
Alw44I 125 GTGCAC G^TGCAC
AlwNI 952 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 848 GGGCCC GGGCC^C
ApaLI 125 GTGCAC G^TGCAC
AspHI 125 GTGCAC G[AT]GC[AT]^C
BalI 41 TGGCCA TGG^CCA
BanII 848 GGGCCC G[GA]GC[CT]^C
BbsI 225 GTCTTC GAAGAC(2/6)
BbvCI 403 GCTGAGG CCTCAGC(-5/-2)
BcnI 120 CCGGG CC^[GC]GG
BglI 28 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 225 GTCTTC GAAGAC(2/6)
BsaHI 109 GACGCC G[GA]^CG[CT]C
BsaWI 1181 TCCGGA [AT]^CCGG[AT]
BseAI 1181 TCCGGA T^CCGGA
BsgI 787 GTGCAG GTGCAG(16/14)
BsiHKAI 125 GTGCAC G[AT]GC[AT]^C
BsmAI 1052 GAGAC GTCTC(1/5)
BsmBI 1052 GAGACG CGTCTC(1/5)
BsmFI 159 GGGAC GGGAC(10/14)
BsmI 414 GAATGC GAATGC(1/-1)
BspEI 1181 TCCGGA T^CCGGA
BspHI 477 TCATGA T^CATGA
BspMI 173 ACCTGC ACCTGC(4/8)
BsrBI 966 CCGCTC CCGCTC(-3/-3)
BsrFI 1090 GCCGGT [GA]^CCGG[CT]
BstAPI 548 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 219 CGCG CG^CG
BstXI 1073 CCAAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1090 GCCGGT [GA]^CCGG[CT]
DsaI 336 CCACGG C^C[GA][CT]GG
EaeI 41 TGGCCA [CT]^GGCC[GA]
HaeII 36 GGCGCT [GA]GCGC^[CT]
Hin1I 109 GACGCC G[GA]^CG[CT]C
HincII 802 GTTAAC GT[CT]^[GA]AC
HindII 802 GTTAAC GT[CT]^[GA]AC
HinfI 1178 GAATC G^A[ACGT]TC
HpaI 802 GTTAAC GTT^AAC
MluNI 41 TGGCCA TGG^CCA
MroI 1181 TCCGGA T^CCGGA
MscI 41 TGGCCA TGG^CCA
MspA1I 64 CCGCTG C[AC]G^C[GT]G
MvnI 219 CGCG CG^CG
NciI 120 CCGGG CC^[GC]GG
PflMI 150 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1000 CTGCAG CTGCA^G
RcaI 477 TCATGA T^CATGA
SapI 304 GAAGAGC GCTCTTC(1/4)
SfcI 1000 CTGCAG C^T[GA][CT]AG
SmlI 614 CTCAAG C^T[CT][GA]AG
TfiI 1178 GAATC G^A[AT]TC
Van91I 150 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1163 CCAACTTCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BmyI 125 GTGCAC G[AGT]GC[ACT]^C
848 GGGCCC
BseRI 1006 CTCCTC GAGGAG(10/8)
341 GAGGAG
Bsp1286I 125 GTGCAC G[AGT]GC[ACT]^C
848 GGGCCC
Bsp1407I 391 TGTACA T^GTACA
909 TGTACA
BsrGI 391 TGTACA T^GTACA
909 TGTACA
BsrI 860 ACTGG ACTGG(1/-1)
664 CCAGT
BstEII 243 GGTGACC G^GT[ACGT]ACC
1101 GGTGACC
BstPI 243 GGTGACC G^GT[ACGT]ACC
1101 GGTGACC
CfoI 37 GCGC GCG^C
218 GCGC
DraII 288 AGGCCCT [GA]G^G[ACGT]CC[CT]
847 AGGGCCC
EarI 304 GAAGAG CTCTTC(1/4)
829 GAAGAG
EcoO109I 288 AGGCCCT [GA]G^G[ACGT]CC[CT]
847 AGGGCCC
EcoO65I 243 GGTGACC G^GT[ACGT]ACC
1101 GGTGACC
EcoT14I 353 CCTTGG C^C[AT][AT]GG
597 CCTTGG
HgaI 109 GACGC GACGC(5/10)
323 GCGTC
HhaI 37 GCGC GCG^C
218 GCGC
HinP1I 37 GCGC G^CGC
218 GCGC
HindIII 650 AAGCTT A^AGCTT
674 AAGCTT
Ksp632I 304 GAAGAG CTCTTC(1/4)
829 GAAGAG
MaeII 53 ACGT A^CGT
1030 ACGT
NlaIV 73 GGCTCC GG[ACGT]^[ACGT]CC
848 GGGCCC
SspBI 391 TGTACA T^GTACA
909 TGTACA
StyI 353 CCTTGG C^C[AT][AT]GG
597 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BsaJI BsiYI
BslI BsrDI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII RsaI
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp45I Tsp509I TspRI